HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Datta, B. Articles by Lengyel, P. Search for Related Content PubMed PubMed Citation Articles by Datta, B. Articles by Lengyel, P. Social Bookmarking                      What's this?

Volume 271, Number 44, Issue of November 1, 1996 pp. 27544-27555
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

p202, an Interferon-inducible Modulator of Transcription, Inhibits Transcriptional Activation by the p53 Tumor Suppressor Protein, and a Segment from the p53-binding Protein 1 That Binds to p202 Overcomes This Inhibition

(Received for publication, July 3, 1996, and in revised form, July 31, 1996)

Bansidhar Datta , Bin Li ^§ , Divaker Choubey , Girish Nallur and Peter Lengyel

From the  Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, the ^§ Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794, and the  Boyer Center of Molecular Medicine and the Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520

p202, an interferon-inducible murine protein, is a member of the ``200 family'' of proteins and is primarily nuclear. p202 is a modulator of transcription; it binds several transcription factors, including NF-B p50 and p65, AP-1 c-Fos and c-Jun, and E2F1, and inhibits their transcriptional activity. p202 also binds pRb, the retinoblastoma protein, and if overexpressed it retards cell proliferation. Here we report that using the yeast two-hybrid assay we found that p202 bound the murine homolog of the human p53-binding protein 1 (53BP1), a protein shown to interact with the DNA binding domain of the p53 tumor suppressor protein. p202 bound a 98amino acid segment from 53BP1. This binding was inhibited by the replacement in p202 of a histidine (from the M(F/L)HATVA(T/S) sequence that is conserved among all of the 200 family proteins) by phenylalanine. We also report that overexpression of p202 inhibited the p53-dependent expression of reporter genes containing p53-activable segments from the mdm2 and p21 genes, whereas a decrease in the p202 level (in consequence of the expression of 202 antisense RNA) resulted in an increase in the p53-dependent expression of these reporters. Expression of the 53BP1 segment binding to p202 overcame the inhibition by overexpressed p202 of the transcription of reporters mediated by the p53 or the AP-1 transcription factors and of the proliferation of yeast.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. R. Gubbels Bupp, M. Li, A. Pashine, D. Aud, and S. L. Peng The candidate lupus susceptibility gene Ifi202a is largely dispensable for B-cell function Rheumatology, January 1, 2008; 47(1): 103 - 104. [Full Text] [PDF]

				M. Yamauchi, M. Hashimoto, K. Ichiyama, R. Yoshida, T. Hanada, T. Muta, S. Komune, T. Kobayashi, and A. Yoshimura Ifi202, an IFN-inducible candidate gene for lupus susceptibility in NZB/W F1 mice, is a positive regulator for NF-{kappa}B activation in dendritic cells Int. Immunol., August 16, 2007; (2007) dxm054v1. [Abstract] [Full Text] [PDF]

				B. Ding, C.-j. Liu, Y. Huang, R. P. Hickey, J. Yu, W. Kong, and P. Lengyel p204 Is Required for the Differentiation of P19 Murine Embryonal Carcinoma Cells to Beating Cardiac Myocytes: ITS EXPRESSION IS ACTIVATED BY THE CARDIAC GATA4, NKX2.5, AND TBX5 PROTEINS J. Biol. Chem., May 26, 2006; 281(21): 14882 - 14892. [Abstract] [Full Text] [PDF]

				H. Xin, S. D'Souza, T. N. Jorgensen, A. T. Vaughan, P. Lengyel, B. L. Kotzin, and D. Choubey Increased Expression of Ifi202, an IFN-Activatable Gene, in B6.Nba2 Lupus Susceptible Mice Inhibits p53-Mediated Apoptosis J. Immunol., May 15, 2006; 176(10): 5863 - 5870. [Abstract] [Full Text] [PDF]

				Y. Ding, J.-F. Lee, H. Lu, M.-H. Lee, and D.-H. Yan Interferon-Inducible Protein IFIX{alpha}1 Functions as a Negative Regulator of HDM2. Mol. Cell. Biol., March 1, 2006; 26(5): 1979 - 1996. [Abstract] [Full Text] [PDF]

				J. M. Dermott, J. M. Gooya, B. Asefa, S. R. Weiler, M. Smith, and J. R. Keller Inhibition of Growth by p205: A Nuclear Protein and Putative Tumor Suppressor Expressed during Myeloid Cell Differentiation Stem Cells, September 1, 2004; 22(5): 832 - 848. [Abstract] [Full Text] [PDF]

				N. Fujiuchi, J. A. Aglipay, T. Ohtsuka, N. Maehara, F. Sahin, G. H. Su, S. W. Lee, and T. Ouchi Requirement of IFI16 for the Maximal Activation of p53 Induced by Ionizing Radiation J. Biol. Chem., May 7, 2004; 279(19): 20339 - 20344. [Abstract] [Full Text] [PDF]

				R. Pramanik, T. N. Jorgensen, H. Xin, B. L. Kotzin, and D. Choubey Interleukin-6 Induces Expression of Ifi202, an Interferon-inducible Candidate Gene for Lupus Susceptibility J. Biol. Chem., April 16, 2004; 279(16): 16121 - 16127. [Abstract] [Full Text] [PDF]

				X.-Y. Ma, H. Wang, B. Ding, H. Zhong, S. Ghosh, and P. Lengyel The Interferon-inducible p202a Protein Modulates NF-{kappa}B Activity by Inhibiting the Binding to DNA of p50/p65 Heterodimers and p65 Homodimers While Enhancing the Binding of p50 Homodimers J. Biol. Chem., June 13, 2003; 278(25): 23008 - 23019. [Abstract] [Full Text] [PDF]

				C.-j. Liu, B. Ding, H. Wang, and P. Lengyel The MyoD-Inducible p204 Protein Overcomes the Inhibition of Myoblast Differentiation by Id Proteins Mol. Cell. Biol., May 1, 2002; 22(9): 2893 - 2905. [Abstract] [Full Text] [PDF]

				W. S. Joo, P. D. Jeffrey, S. B. Cantor, M. S. Finnin, D. M. Livingston, and N. P. Pavletich Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure Genes & Dev., March 1, 2002; 16(5): 583 - 593. [Abstract] [Full Text] [PDF]

				D. Jullien, P. Vagnarelli, W. C. Earnshaw, and Y. Adachi Kinetochore localisation of the DNA damage response component 53BP1 during mitosis J. Cell Sci., January 1, 2002; 115(1): 71 - 79. [Abstract] [Full Text] [PDF]

				C.-j. Liu, H. Wang, Z. Zhao, S. Yu, Y.-b. Lu, J. Meyer, G. Chatterjee, S. Deschamps, B. A. Roe, and P. Lengyel MyoD-Dependent Induction during Myoblast Differentiation of p204, a Protein Also Inducible by Interferon Mol. Cell. Biol., September 15, 2000; 20(18): 7024 - 7036. [Abstract] [Full Text]

				Y. Geng, S. D’Souza, H. Xin, S. Walter, and D. Choubey p202 Levels Are Negatively Regulated by Serum Growth Factors Cell Growth Differ., September 1, 2000; 11(9): 475 - 483. [Abstract] [Full Text]

				Y. Wen, D.-H. Yan, B. Spohn, J. Deng, S.-Y. Lin, and M.-C. Hung Tumor Suppression and Sensitization to Tumor Necrosis Factor {{alpha}}-induced Apoptosis by an Interferon-inducible Protein, p202, in Breast Cancer Cells Cancer Res., January 1, 2000; 60(1): 42 - 46. [Abstract] [Full Text]

				R. W. Johnstone and J. A. Trapani Transcription and Growth Regulatory Functions of the HIN-200 Family of Proteins Mol. Cell. Biol., September 1, 1999; 19(9): 5833 - 5838. [Full Text] [PDF]

				A. R. Cuddihy, S. Li, N. W. N. Tam, A. H.-T. Wong, Y. Taya, N. Abraham, J. C. Bell, and A. E. Koromilas Double-Stranded-RNA-Activated Protein Kinase PKR Enhances Transcriptional Activation by Tumor Suppressor p53 Mol. Cell. Biol., April 1, 1999; 19(4): 2475 - 2484. [Abstract] [Full Text] [PDF]

				J. U. Gutterman and D. Choubey Retardation of Cell Proliferation after Expression of p202 Accompanies an Increase in p21WAF1/CIP1 Cell Growth Differ., February 1, 1999; 10(2): 93 - 100. [Abstract] [Full Text]

				S. R. Weiler, J. M. Gooya, M. Ortiz, S. Tsai, S. J. Collins, and J. R. Keller D3: A Gene Induced During Myeloid Cell Differentiation of Linlo c-Kit+ Sca-1+ Progenitor Cells Blood, January 15, 1999; 93(2): 527 - 536. [Abstract] [Full Text] [PDF]

				K. Iwabuchi, B. Li, H. F. Massa, B. J. Trask, T. Date, and S. Fields Stimulation of p53-mediated Transcriptional Activation by the p53-binding Proteins, 53BP1 and 53BP2 J. Biol. Chem., October 2, 1998; 273(40): 26061 - 26068. [Abstract] [Full Text] [PDF]

				R. W. Johnstone, J. A. Kerry, and J. A. Trapani The Human Interferon-inducible Protein, IFI 16, Is a Repressor of Transcription J. Biol. Chem., July 3, 1998; 273(27): 17172 - 17177. [Abstract] [Full Text] [PDF]

				B. Datta, W. Min, S. Burma, and P. Lengyel Increase in p202 Expression during Skeletal Muscle Differentiation: Inhibition of MyoD Protein Expression and Activity by p202 Mol. Cell. Biol., February 1, 1998; 18(2): 1074 - 1083. [Abstract] [Full Text]

				H. Wang, C.-j. Liu, Y. Lu, G. Chatterjee, X.-Y. Ma, R. N. Eisenman, and P. Lengyel The Interferon- and Differentiation-inducible p202a Protein Inhibits the Transcriptional Activity of c-Myc by Blocking Its Association with Max J. Biol. Chem., August 25, 2000; 275(35): 27377 - 27385. [Abstract] [Full Text] [PDF]

				S. D'Souza, H. Xin, S. Walter, and D. Choubey The Gene Encoding p202, an Interferon-inducible Negative Regulator of the p53 Tumor Suppressor, Is a Target of p53-mediated Transcriptional Repression J. Biol. Chem., January 5, 2001; 276(1): 298 - 305. [Abstract] [Full Text] [PDF]

				Z. Xia, J. C. Morales, W. G. Dunphy, and P. B. Carpenter Negative Cell Cycle Regulation and DNA Damage-inducible Phosphorylation of the BRCT Protein 53BP1 J. Biol. Chem., January 19, 2001; 276(4): 2708 - 2718. [Abstract] [Full Text] [PDF]