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Volume 271, Number 44, Issue of November 1, 1996 pp. 27677-27685
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes

(Received for publication, April 29, 1996, and in revised form, July 8, 1996)

Diane A. Blake , Pampa Chakrabarti ^¶ , Mehraban Khosraviani , Frank M. Hatcher ^¶ , Connie M. Westhoff , Peter Goebel , Dwane E. Wylie and Robert C. Blake II^''

From the  Department of Ophthalmology, Tulane University School of Medicine, New Orleans, Louisiana 70112, the ^¶ Department of Microbiology, Meharry Medical College, Nashville, Tennessee 37208, the  Department of Biology, University of Nebraska, Lincoln, Nebraska 68588, and the ^'' Department of Basic Pharmaceutical Sciences, Xavier University of Louisiana, New Orleans, Louisiana 70125

A monoclonal antibody that recognizes cadmium-EDTA complexes has been produced by the injection of BALB/c mice with a metal-chelate complex covalently coupled to a carrier protein. The ability of purified antibody to recognize 16 different metal-EDTA complexes was assessed by measuring equilibrium binding constants using a KinExA^TM immunoassay instrument. The antibody bound to cadmium- and mercury-EDTA complexes with equilibrium dissociation constants of 21 and 26 nM, respectively. All other metal-EDTA complexes tested, including those of Mn(II), In(III), Ni(II), Zn(II), Co(II), Cu(II), Ag(I), Fe(III), Pb(II), Au(III), Tb(III), Ga(III), Mg(II), and Al(III) bound with affinities from 20- to 40,000-fold less than that determined for the cadmium-EDTA complex. With the exception of mercury and magnesium, the binding of divalent metal-chelate complexes was well-correlated with the size of the metal ion. The amino acid sequences of the heavy and light chain variable regions were deduced from polymerase chain reaction-amplified regions of the corresponding genes and subsequently used to construct molecular models of the antigen binding region. The key residue for cadmium binding in the model for 2A81G5 appeared to be histidine 96 in the heavy chain.

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