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Volume 271, Number 45, Issue of November 8, 1996 pp. 27987-27990
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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COMMUNICATION:
Evidence for Involvement of Yeast Proliferating Cell Nuclear Antigen in DNA Mismatch Repair

(Received for publication, August 12, 1996, and in revised form, September 13, 1996)

Robert E. Johnson , Gopala K. Kovvali , Sami N. Guzder , Neelam S. Amin ^§ , Connie Holm ^§ , Yvette Habraken , Patrick Sung , Louise Prakash and Satya Prakash

From the  Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061 and the ^§ Department of Pharmacology, Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093-0651

DNA mismatch repair plays a key role in the maintenance of genetic fidelity. Mutations in the human mismatch repair genes hMSH2, hMLH1, hPMS1, and hPMS2 are associated with hereditary nonpolyposis colorectal cancer. The proliferating cell nuclear antigen (PCNA) is essential for DNA replication, where it acts as a processivity factor. Here, we identify a point mutation, pol30-104, in the Saccharomyces cerevisiae POL30 gene encoding PCNA that increases the rate of instability of simple repetitive DNA sequences and raises the rate of spontaneous forward mutation. Epistasis analyses with mutations in mismatch repair genes MSH2, MLH1, and PMS1 suggest that the pol30-104 mutation impairs MSH2/MLH1/PMS1-dependent mismatch repair, consistent with the hypothesis that PCNA functions in mismatch repair. MSH2 functions in mismatch repair with either MSH3 or MSH6, and the MSH2-MSH3 and MSH2-MSH6 heterodimers have a role in the recognition of DNA mismatches. Consistent with the genetic data, we find specific interaction of PCNA with the MSH2-MSH3 heterodimer.

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