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Volume 271, Number 45,
Issue of November 8, 1996
pp. 28189-28198
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Mass Spectrometric Analysis of 40 S Ribosomal Proteins from
Rat-1 Fibroblasts
(Received for publication, May 23, 1996, and in revised form, August 8, 1996)
Donna F.
Louie
§
,
Katheryn A.
Resing
§
,
Timothy S.
Lewis
§
and
Natalie G.
Ahn
§
From the Howard Hughes Medical Institute and the
§ Department of Chemistry and Biochemistry, University of
Colorado, Boulder, Colorado 80309
Although sequences of most mammalian ribosomal
proteins are available, little is known about the post-translational
processing of ribosomal proteins. To examine their post-translational
modifications, 40 S subunit proteins purified from Rat-1 fibroblasts
and their peptides were analyzed by liquid chromatography coupled with
electrospray mass spectrometry. Of 41 proteins observed, 36
corresponded to the 32 rat 40 S ribosomal proteins with known sequences
(S3, S5, S7, and S24 presented in two forms). The observed masses of
S4, S6-S8, S13, S15a, S16, S17, S19, S27a, S29, and S30 matched those
predicted. Sa, S3a, S5, S11, S15, S18, S20, S21, S24, S26-S28, and an
S7 variant showed changes in mass that were consistent with N-terminal
demethionylation and/or acetylation (S5 and S27 also appeared to be
internally formylated and acetylated, respectively). S23 appeared to be
internally hydroxylated or methylated. S2, S3, S9, S10, S12, S14, and
S25 showed changes in mass inconsistent with known covalent
modifications (+220, 75, +86, +56, 100, 117, and 103 Da,
respectively), possibly representing novel post-translational
modifications or allelic sequence variation. Five unidentified proteins
(12,084, 13,706, 13,741, 13,884, and 34,987 Da) were observed; for one,
a sequence tag (PPGPPP), absent in any known ribosomal proteins, was
determined, suggesting that it is a previously undescribed
ribosome-associated protein. This study establishes a powerful method
to rapidly analyze protein components of large biological complexes and
their covalent modifications.

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Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.
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