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Volume 271, Number 45, Issue of November 8, 1996 pp. 28266-28270
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Synthesis of Full-length Viral DNA in CD4-positive Membrane Vesicles Exposed to HIV-1
A MODEL FOR STUDIES OF EARLY STAGES OF THE HIV-1 LIFE CYCLE

(Received for publication, March 12, 1996, and in revised form, August 20, 1996)

From the Molecular Virology Laboratory, St. Luke's-Roosevelt Hospital Center and College of Physicians & Surgeons, Columbia University, New York, New York 10019

CD4-positive membrane vesicles (MV) were isolated under isotonic conditions from human T lymphoblastoid cells MT-2 and CEM and tested for their ability to support reverse transcription of viral RNA upon exposure to human immunodeficiency virus, type 1 (HIV-1). MV contained cytoplasms as confirmed by the presence of mitochondrial DNA but were devoid of chromosomal DNA. Virus binding and vesicle lysis assays revealed that 4-19% (depending upon virus dose) of MV-bound HIV-1 entered the vesicles. HIV-1 internalized in MV was able to initiate and complete viral DNA synthesis as determined by the detection of products of reverse transcription using polymerase chain reaction amplification of viral DNA using regions present in early (strong stop) transcripts and full-length double-stranded molecules. Viral DNA was undetectable in MV exposed to HIV-1 at 0 °C, in MV exposed to UV-inactivated virus at 37 °C, or after exposure to intact virus at 37 °C in the presence of reverse transcriptase inhibitors 2,3-dideoxycytidine and a tetrahydroimidazo[4,5,1-jk](1,4)-benzodiazepin-2-(1H)-thione derivative, indicating that viral DNA detected in HIV-1-exposed MV was synthesized de novo. Kinetic studies revealed that HIV-1 DNA synthesis in MV was very rapid; full-length viral DNA was detected within 15 min of exposure at 37 °C, and the DNA levels increased 90-fold after 1 h and declined thereafter. Strong stop viral DNA was 10-fold more abundant than full-length DNA after 1 h at 37 °C, indicating that 10% of input viral genomes are fully transcribed in MV within this time frame. This system preserves the critical features of intact CD4-bearing cells to permit studies of HIV-1 entry, uncoating, and reverse transcription of viral RNA.

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