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Volume 271, Number 45, Issue of November 8, 1996 pp. 28439-28444
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Histidine Decarboxylase Expression in Mouse Mast Cell Line P815 Is Induced by Mouse Peritoneal Cavity Incubation

(Received for publication, August 2, 1996)

Hiroshi Ohtsu , Atsuo Kuramasu , Satsuki Suzuki , Kazuhiko Igarashi ^¶ , Yuzuru Ohuchi , Maki Sato , Satoshi Tanaka , Satoshi Nakagawa , Kunio Shirato ^** , Masayuki Yamamoto ^¶ , Atsushi Ichikawa and Takehiko Watanabe

From the Departments of  Pharmacology I and ^** Internal Medicine I, Tohoku University School of Medicine, Sendai 980-77, the ^¶ Division of Molecular and Developmental Biology, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305, and the  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-01, Japan

Phenotype of P815 mouse mast cells changes markedly during culture in the peritoneal cavity of syngenic BDF1 mice. The cells, cultured for 1 week in the peritoneal cavity of syngenic BDF1 mice, proliferate and express high levels of L-histidine decarboxylase (HDC) and mouse mast cell protease (MMCP)-6 mRNAs, indicating the ability of P815 cells to differentiate toward mature connective tissue mast cells. Peritoneal fluid aspirated from P815-inoculated BDF1 mouse and added to cultured P815 cells in vitro was also found to induce HDC mRNA expression, suggesting that at least some of the humoral factors in the peritoneal fluid induce HDC mRNA transcription. Among the erythroid transcription factors, P815 cells expressed GATA-2 but not GATA-1 mRNA before and after the intraperitoneal incubation. In contrast, the expression of NF-E2 subunit p45 disappeared, while expression of subunit mafK was markedly reduced after incubation. Cotransfection assays using HDC-luciferase reporter and p45 and/or mafK expression constructs showed that NF-E2 affects the transactivation of HDC gene. These results suggest that NF-E2 is also an important transcription factor in mast cell differentiation.

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