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(Received for publication, July 12, 1996, and in revised form, August 26, 1996)
From the Neuroscience Center and Department of Ophthalmology,
Louisiana State University Medical Center,
New Orleans, Louisiana 70112
Expression of early response genes during rod
outer segment phagocytosis by normal Long Evans and Royal College of
Surgeons-rdy+p+ rats
and by dystrophic Royal College of Surgeons-p+
rat retinal pigment epithelial cells was studied in primary cell
culture. Northern analysis revealed that the abundance of
zif-268 (egr-1), c-fos, and
tis-1 (NGF1-B) mRNA was rapidly and
transiently increased in normal retinal pigment epithelial cells during
rod outer segment phagocytosis but not during phagocytosis of latex
particles. No increase in gene expression was found in Royal College of
Surgeons-p+ dystrophic retinal pigment
epithelial cells challenged with rod outer segments. As shown by
electrophoretic mobility shift assay, a prominent short term increase
in the intensity of the gel-shifted band was detected using nuclear
protein extracts derived from rod outer segment-challenged, control
retinal pigment epithelial cells and zif-268, AP-1, AP-2,
or tis-1 consensus oligonucleotides. No such increase was
detected when using nuclear factor
Volume 271, Number 45,
Issue of November 8, 1996
pp. 28458-28462
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
B consensus oligonucleotide or
when the early response gene prostaglandin H synthase-2 mRNA was
measured over the time course studied. The results suggest that in
retinal pigment epithelial cells, rod outer segment-specific
phagocytosis is accompanied by the selective expression of early
response genes coding for transcription factors. The specific pattern
of the induction of these transcription factors is predicted to
modulate the expression of gene cascades.
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