HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ogier-Denis, E. Articles by Codogno, P. Search for Related Content PubMed PubMed Citation Articles by Ogier-Denis, E. Articles by Codogno, P. Social Bookmarking                      What's this?

Volume 271, Number 45, Issue of November 8, 1996 pp. 28593-28600
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Guanine Nucleotide Exchange on Heterotrimeric G_i3 Protein Controls Autophagic Sequestration in HT-29 Cells

(Received for publication, April 1, 1996, and in revised form, August 27, 1996)

From INSERM U410 Neuroendocrinologie et Biologie Cellulaire Digestives, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France

Recent results have shown that autophagic sequestration in the human colon cancer cell line HT-29 is controlled by the pertussis toxin-sensitive heterotrimeric G_i3 protein. Here we show that transfection of an antisense oligodeoxynucleotide to the _i3-subunit markedly inhibits autophagic sequestration, whereas transfection of an antisense oligodeoxynucleotide to the _i2-subunit does not change the rate of autophagy in HT-29 cells. Autophagic sequestration was arrested in cells transfected with a mutant of the _i3-subunit (Q204L) that is restricted to the GTP-bound form. In Q204L-expressing cells, 3-methyladenine-sensitive degradation of long lived [¹⁴C]valine-labeled proteins was severely impaired and could not be stimulated by nutrient deprivation. Autophagy was also reduced when dissociation of the dimer from the GTP-bound _i3-subunit was impaired in cells transfected with the G203A mutant. In contrast, a high rate of pertussis toxin-sensitive autophagy was observed in cells transfected with an _i3-subunit mutant (S47N) which has an increased guanine nucleotide exchange rate and increased preference for GDP over GTP. Cells that express pertussis toxin-insensitive mutants of either wild-type _i3-subunit (C351S) or S47N _i3-subunit (S47N/C351S) exhibit a high rate of autophagy.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Sato, K. Tsuchihara, S. Fujii, M. Sugiyama, T. Goya, Y. Atomi, T. Ueno, A. Ochiai, and H. Esumi Autophagy Is Activated in Colorectal Cancer Cells and Contributes to the Tolerance to Nutrient Deprivation Cancer Res., October 15, 2007; 67(20): 9677 - 9684. [Abstract] [Full Text] [PDF]

				E. Y. W. Chan, S. Kir, and S. A. Tooze siRNA Screening of the Kinome Identifies ULK1 as a Multidomain Modulator of Autophagy J. Biol. Chem., August 31, 2007; 282(35): 25464 - 25474. [Abstract] [Full Text] [PDF]

				H. Shorer, N. Amar, A. Meerson, and Z. Elazar Modulation of N-Ethylmaleimide-sensitive Factor Activity upon Amino Acid Deprivation J. Biol. Chem., April 22, 2005; 280(16): 16219 - 16226. [Abstract] [Full Text] [PDF]

				S. L. Fink and B. T. Cookson Apoptosis, Pyroptosis, and Necrosis: Mechanistic Description of Dead and Dying Eukaryotic Cells Infect. Immun., April 1, 2005; 73(4): 1907 - 1916. [Full Text] [PDF]

				P. Boya, R.-A. Gonzalez-Polo, N. Casares, J.-L. Perfettini, P. Dessen, N. Larochette, D. Metivier, D. Meley, S. Souquere, T. Yoshimori, et al. Inhibition of Macroautophagy Triggers Apoptosis Mol. Cell. Biol., February 1, 2005; 25(3): 1025 - 1040. [Abstract] [Full Text] [PDF]

				S. Pattingre, L. De Vries, C. Bauvy, I. Chantret, F. Cluzeaud, E. Ogier-Denis, A. Vandewalle, and P. Codogno The G-protein Regulator AGS3 Controls an Early Event during Macroautophagy in Human Intestinal HT-29 Cells J. Biol. Chem., May 30, 2003; 278(23): 20995 - 21002. [Abstract] [Full Text] [PDF]

				S. Pattingre, C. Bauvy, and P. Codogno Amino Acids Interfere with the ERK1/2-dependent Control of Macroautophagy by Controlling the Activation of Raf-1 in Human Colon Cancer HT-29 Cells J. Biol. Chem., May 2, 2003; 278(19): 16667 - 16674. [Abstract] [Full Text] [PDF]

				D. B. Munafo and M. I. Colombo A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation J. Cell Sci., March 12, 2002; 114(20): 3619 - 3629. [Abstract] [Full Text] [PDF]

				M. Nagahama, S. Usui, T. Shinohara, T. Yamaguchi, K. Tani, and M. Tagaya Inactivation of G{alpha}z causes disassembly of the Golgi apparatus J. Cell Sci., January 12, 2002; 115(23): 4483 - 4493. [Abstract] [Full Text] [PDF]

				N. Mizushima, A. Yamamoto, M. Hatano, Y. Kobayashi, Y. Kabeya, K. Suzuki, T. Tokuhisa, Y. Ohsumi, and T. Yoshimori Dissection of Autophagosome Formation using Apg5-deficient Mouse Embryonic Stem Cells J. Cell Biol., February 12, 2001; 152(4): 657 - 668. [Abstract] [Full Text] [PDF]

				D. J. Klionsky and S. D. Emr Autophagy as a Regulated Pathway of Cellular Degradation Science, December 1, 2000; 290(5497): 1717 - 1721. [Abstract] [Full Text]

				M. H. Ghahremani, C. Forget, and P. R. Albert Distinct Roles for Galpha i2 and Gbeta gamma in Signaling to DNA Synthesis and Galpha i3 in Cellular Transformation by Dopamine D2S Receptor Activation in BALB/c 3T3 Cells Mol. Cell. Biol., March 1, 2000; 20(5): 1497 - 1506. [Abstract] [Full Text]

				A. Petiot, E. Ogier-Denis, E. F. C. Blommaart, A. J. Meijer, and P. Codogno Distinct Classes of Phosphatidylinositol 3'-Kinases Are Involved in Signaling Pathways That Control Macroautophagy in HT-29 Cells J. Biol. Chem., January 14, 2000; 275(2): 992 - 998. [Abstract] [Full Text] [PDF]

				K. Shigemitsu, Y. Tsujishita, K. Hara, M. Nanahoshi, J. Avruch, and K. Yonezawa Regulation of Translational Effectors by Amino Acid and Mammalian Target of Rapamycin Signaling Pathways. POSSIBLE INVOLVEMENT OF AUTOPHAGY IN CULTURED HEPATOMA CELLS J. Biol. Chem., January 8, 1999; 274(2): 1058 - 1065. [Abstract] [Full Text] [PDF]

				E. Ogier-Denis, A. Petiot, C. Bauvy, and P. Codogno Control of the Expression and Activity of the Galpha -interacting Protein (GAIP) in Human Intestinal Cells J. Biol. Chem., September 26, 1997; 272(39): 24599 - 24603. [Abstract] [Full Text] [PDF]

				S. Arico, A. Petiot, C. Bauvy, P. F. Dubbelhuis, A. J. Meijer, P. Codogno, and E. Ogier-Denis The Tumor Suppressor PTEN Positively Regulates Macroautophagy by Inhibiting the Phosphatidylinositol 3-Kinase/Protein Kinase B Pathway J. Biol. Chem., September 14, 2001; 276(38): 35243 - 35246. [Abstract] [Full Text] [PDF]

				E. Ogier-Denis, S. Pattingre, J. El Benna, and P. Codogno Erk1/2-dependent Phosphorylation of Galpha -interacting Protein Stimulates Its GTPase Accelerating Activity and Autophagy in Human Colon Cancer Cells J. Biol. Chem., December 8, 2000; 275(50): 39090 - 39095. [Abstract] [Full Text] [PDF]