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Volume 271, Number 45, Issue of November 8, 1996 pp. 28624-28629
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Inhibition of Tumor Necrosis Factor Signal Transduction in Endothelial Cells by Dimethylaminopurine

(Received for publication, July 12, 1996)

Michael W. Marino ^§ , James D. Dunbar ^¶ , Li-Wha Wu ^¶ , Justinian R. Ngaiza , Hyung-Mee Han , Danqun Guo ^¶ , Masayuki Matsushita ^** , Angus C. Nairn ^** , Yuhua Zhang , Richard Kolesnick , Eric A. Jaffe and David B. Donner ^¶

From the  Memorial Sloan-Kettering Cancer Center and the ^§ Ludwig Institute for Cancer Research, New York Branch, the ^** Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, and the  Division of Hematology/Oncology, Department of Medicine and Specialized Center of Research in Thrombosis, Cornell University Medical College, New York, New York 10021 and the ^¶ Department of Physiology and Biophysics and the Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202

Tumor necrosis factor (TNF) promotes diverse responses in endothelial cells that are important to the host response to infections and malignancies; however, less is known of the postreceptor events important to TNF action in endothelial cells than in many other cell types. Since phosphorylation cascades are implicated in cytokine signaling, the effects of the protein kinase inhibitor dimethylaminopurine (DMAP) on TNF action in bovine aortic endothelial cells (BAEC) were investigated. In BAEC, TNF promotes phosphorylation of eukaryotic initiation factor 4E (eIF-4E), c-Jun N-terminal kinase (JNK) and ceramide-activated protein kinase activities, Jun-b expression, prostacyclin production, and, when protein synthesis is inhibited, cytotoxicity. DMAP abrogated or significantly attenuated each of these responses to TNF, without affecting the specific binding of TNF to its receptors. Histamine, another agent active in the endothelium, promotes phosphorylation of elongation factor-2 (EF-2) and prostacyclin production, but not phosphorylation of eIF-4E in BAEC. Histamine-stimulated EF-2 phosphorylation was not inhibited and prostacyclin production was unaffected by DMAP. These observations demonstrate that a distinct signal transduction cascade, which can be selectively inhibited by DMAP, promotes the response of BAEC to TNF. Thus, we have identified a reagent, DMAP, that may be useful for characterizing the TNF signal transduction pathway.

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