| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 25, 1996, and in revised form, August 16, 1996)
From the The regulation of human androgen receptor (AR) by
the molecular chaperone Hsp90 was investigated using the yeast
Saccharomyces cerevisiae as a model system. These studies
were performed in strains expressing a conditional
temperature-sensitive mutant allele of the hsp82 gene,
which encodes Hsp90 protein. At the restrictive temperature in the
mutant, there is a decrease in hormone-dependent
transactivation by the AR, although steady state levels of AR protein
are unchanged. Quantitative hormone binding studies at the permissive
temperature revealed the presence of both high affinity and low
affinity hormone binding states. At the restrictive temperature in the
hsp82 mutant, the high affinity state was abolished, and
only the low affinity state was observed. The change in hormone binding
affinity was further investigated by a competition assay with the
anti-androgen hydroxyflutamide. Under permissive conditions,
hydroxyflutamide competes poorly for the synthetic androgen R1881, but
under restrictive conditions in the hsp82 mutant strain,
hydroxyflutamide was shown to be a potent competitive inhibitor. Our
findings indicate that Hsp90 participates in the activation process by
maintaining apoAR in a high affinity ligand binding conformation which
is important for efficient response to hormone.
Volume 271, Number 45,
Issue of November 8, 1996
pp. 28697-28702
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
Department of Cell Biology and Anatomy,
Mount Sinai Medical Center, New York New York 10029 and
§ Department of Human Genetics, University of Michigan
Medical School, Ann Arbor, Michigan 48109
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. F. Smith and D. O. Toft Minireview: The Intersection of Steroid Receptors with Molecular Chaperones: Observations and Questions Mol. Endocrinol., October 1, 2008; 22(10): 2229 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Basak, D. Pookot, E. J. Noonan, and R. Dahiya Genistein down-regulates androgen receptor by modulating HDAC6-Hsp90 chaperone function Mol. Cancer Ther., October 1, 2008; 7(10): 3195 - 3202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Belova, D. R. Brickley, B. Ky, S. K. Sharma, and S. D. Conzen Hsp90 Regulates the Phosphorylation and Activity of Serum- and Glucocorticoid-regulated Kinase-1 J. Biol. Chem., July 4, 2008; 283(27): 18821 - 18831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Seaton, P. Scullin, P. J. Maxwell, C. Wilson, J. Pettigrew, R. Gallagher, J. M. O'Sullivan, P. G. Johnston, and D. J. J. Waugh Interleukin-8 signaling promotes androgen-independent proliferation of prostate cancer cells via induction of androgen receptor expression and activation Carcinogenesis, June 1, 2008; 29(6): 1148 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Cutress, H. C. Whitaker, I. G. Mills, M. Stewart, and D. E. Neal Structural basis for the nuclear import of the human androgen receptor J. Cell Sci., April 1, 2008; 121(7): 957 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Kesler, D. Gioeli, M. R. Conaway, M. J. Weber, and B. M. Paschal Subcellular Localization Modulates Activation Function 1 Domain Phosphorylation in the Androgen Receptor Mol. Endocrinol., September 1, 2007; 21(9): 2071 - 2084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li, M. E Andersen, S. Heber, and Q. Zhang Non-monotonic dose-response relationship in steroid hormone receptor-mediated gene expression J. Mol. Endocrinol., May 1, 2007; 38(5): 569 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Banerji, M. Walton, F. Raynaud, R. Grimshaw, L. Kelland, M. Valenti, I. Judson, and P. Workman Pharmacokinetic-Pharmacodynamic Relationships for the Heat Shock Protein 90 Molecular Chaperone Inhibitor 17-Allylamino, 17-Demethoxygeldanamycin in Human Ovarian Cancer Xenograft Models Clin. Cancer Res., October 1, 2005; 11(19): 7023 - 7032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chen, S. Meng, H. Wang, P. Bali, W. Bai, B. Li, P. Atadja, K. N. Bhalla, and J. Wu Chemical ablation of androgen receptor in prostate cancer cells by the histone deacetylase inhibitor LAQ824 Mol. Cancer Ther., September 1, 2005; 4(9): 1311 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Banerji, A. O'Donnell, M. Scurr, S. Pacey, S. Stapleton, Y. Asad, L. Simmons, A. Maloney, F. Raynaud, M. Campbell, et al. Phase I Pharmacokinetic and Pharmacodynamic Study of 17-Allylamino, 17-Demethoxygeldanamycin in Patients With Advanced Malignancies J. Clin. Oncol., June 20, 2005; 23(18): 4152 - 4161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Pajonk, A. van Ophoven, and W. H. McBride Hyperthermia-Induced Proteasome Inhibition and Loss of Androgen Receptor Expression in Human Prostate Cancer Cells Cancer Res., June 1, 2005; 65(11): 4836 - 4843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheung-Flynn, V. Prapapanich, M. B. Cox, D. L. Riggs, C. Suarez-Quian, and D. F. Smith Physiological Role for the Cochaperone FKBP52 in Androgen Receptor Signaling Mol. Endocrinol., June 1, 2005; 19(6): 1654 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Mayeur, W.-J. Kung, A. Martinez, C. Izumiya, D. J. Chen, and H.-J. Kung Ku Is a Novel Transcriptional Recycling Coactivator of the Androgen Receptor in Prostate Cancer Cells J. Biol. Chem., March 18, 2005; 280(11): 10827 - 10833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-L. Cha, L. Qiu, C.-T. Chen, Y. Wen, and M.-C. Hung Emodin Down-Regulates Androgen Receptor and Inhibits Prostate Cancer Cell Growth Cancer Res., March 15, 2005; 65(6): 2287 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Hu, S. Yeh, S.-D. Yeh, E. R. Sampson, J. Huang, P. Li, C.-L. Hsu, H.-J. Ting, H.-K. Lin, L. Wang, et al. Functional Domain and Motif Analyses of Androgen Receptor Coregulator ARA70 and Its Differential Expression in Prostate Cancer J. Biol. Chem., August 6, 2004; 279(32): 33438 - 33446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. He, S. Bai, A. T. Hnat, R. I. Kalman, J. T. Minges, C. Patterson, and E. M. Wilson An Androgen Receptor NH2-terminal Conserved Motif Interacts with the COOH Terminus of the Hsp70-interacting Protein (CHIP) J. Biol. Chem., July 16, 2004; 279(29): 30643 - 30653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang, Y. E. Liu, I. D. Goldberg, and Y. E. Shi {gamma} Synuclein, a Novel Heat-Shock Protein-Associated Chaperone, Stimulates Ligand-Dependent Estrogen Receptor {alpha} Signaling and Mammary Tumorigenesis Cancer Res., July 1, 2004; 64(13): 4539 - 4546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Lee, R. W. Thompson, J. M. McClung, and J. A. Carson Regulation of androgen receptor expression at the onset of functional overload in rat plantaris muscle Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2003; 285(5): R1076 - R1085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ezer and B. Robaire Gene Expression Is Differentially Regulated in the Epididymis after Orchidectomy Endocrinology, March 1, 2003; 144(3): 975 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Morales, E. T. Snow, and J. P. Murnane Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors Mutagenesis, March 1, 2003; 18(2): 151 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liao, L.-Y. Chen, A. Zhang, A. Godavarthy, F. Xia, J. C. Ghosh, H. Li, and J. D. Chen Regulation of Androgen Receptor Activity by the Nuclear Receptor Corepressor SMRT J. Biol. Chem., February 7, 2003; 278(7): 5052 - 5061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Pratt and D. O. Toft Regulation of Signaling Protein Function and Trafficking by the hsp90/hsp70-Based Chaperone Machinery Experimental Biology and Medicine, February 1, 2003; 228(2): 111 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. James, I. U. Agoulnik, J. M. Harris, G. Buchanan, W. D. Tilley, M. Marcelli, D. J. Lamb, and N. L. Weigel A Novel Androgen Receptor Mutant, A748T, Exhibits Hormone Concentration-Dependent Defects in Nuclear Accumulation and Activity Despite Normal Hormone-Binding Affinity Mol. Endocrinol., December 1, 2002; 16(12): 2692 - 2705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Goeckeler, A. Stephens, P. Lee, A. J. Caplan, and J. L. Brodsky Overexpression of Yeast Hsp110 Homolog Sse1p Suppresses ydj1-151 Thermosensitivity and Restores Hsp90-dependent Activity Mol. Biol. Cell, August 1, 2002; 13(8): 2760 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Solit, F. F. Zheng, M. Drobnjak, P. N. Munster, B. Higgins, D. Verbel, G. Heller, W. Tong, C. Cordon-Cardo, D. B. Agus, et al. 17-Allylamino-17-demethoxygeldanamycin Induces the Degradation of Androgen Receptor and HER-2/neu and Inhibits the Growth of Prostate Cancer Xenografts Clin. Cancer Res., May 1, 2002; 8(5): 986 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Heinlein and C. Chang Androgen Receptor (AR) Coregulators: An Overview Endocr. Rev., April 1, 2002; 23(2): 175 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Lee, T. Hon, and L. Zhang The Molecular Chaperone Hsp90 Mediates Heme Activation of the Yeast Transcriptional Activator Hap1 J. Biol. Chem., February 22, 2002; 277(9): 7430 - 7437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Markus, S. S. Taneja, S. K. Logan, W. Li, S. Ha, A. B. Hittelman, I. Rogatsky, and M. J. Garabedian Identification and Characterization of ART-27, a Novel Coactivator for the Androgen Receptor N Terminus Mol. Biol. Cell, February 1, 2002; 13(2): 670 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Knowlton and L. Sun Heat-shock factor-1, steroid hormones, and regulation of heat-shock protein expression in the heart Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H455 - H464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Fliss, J. Rao, M. W. Melville, M. E. Cheetham, and A. J. Caplan Domain Requirements of DnaJ-like (Hsp40) Molecular Chaperones in the Activation of a Steroid Hormone Receptor J. Biol. Chem., November 26, 1999; 274(48): 34045 - 34052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Poukka, P. Aarnisalo, U. Karvonen, J. J. Palvimo, and O. A. Janne Ubc9 Interacts with the Androgen Receptor and Activates Receptor-dependent Transcription J. Biol. Chem., July 2, 1999; 274(27): 19441 - 19446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Sperry and P. Thomas Characterization of Two Nuclear Androgen Receptors in Atlantic Croaker: Comparison of Their Biochemical Properties and Binding Specificities Endocrinology, April 1, 1999; 140(4): 1602 - 1611. [Abstract] [Full Text]
|
|
|
|
|
|
J.-F. Louvion, T. Abbas-Terki, and D. Picard Hsp90 Is Required for Pheromone Signaling in Yeast Mol. Biol. Cell, November 1, 1998; 9(11): 3071 - 3083. [Abstract] [Full Text]
|
|
|
|
|
|
M. V. Govindan and N. Warriar Reconstitution of the N-terminal Transcription Activation Function of Human Mineralocorticoid Receptor in a Defective Human Glucocorticoid Receptor J. Biol. Chem., September 18, 1998; 273(38): 24439 - 24447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Scheller, E. Hughes, K. L. Golden, and D. M. Robins Multiple Receptor Domains Interact to Permit, or Restrict, Androgen-specific Gene Activation J. Biol. Chem., September 11, 1998; 273(37): 24216 - 24222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. Moilanen, U. Karvonen, H. Poukka, O. A. Jänne, and J. J. Palvimo Activation of Androgen Receptor Function by a Novel Nuclear Protein Kinase Mol. Biol. Cell, September 1, 1998; 9(9): 2527 - 2543. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Fang, A. E. Fliss, J. Rao, and A. J. Caplan SBA1 Encodes a Yeast Hsp90 Cochaperone That Is Homologous to Vertebrate p23 Proteins Mol. Cell. Biol., July 1, 1998; 18(7): 3727 - 3734. [Abstract] [Full Text]
|
|
|
|
|
|
B. A. Froesch, S. Takayama, and J. C. Reed BAG-1L Protein Enhances Androgen Receptor Function J. Biol. Chem., May 8, 1998; 273(19): 11660 - 11666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Dittmar, M. Banach, M. D. Galigniana, and W. B. Pratt The Role of DnaJ-like Proteins in Glucocorticoid Receptor·hsp90 Heterocomplex Assembly by the Reconstituted hsp90·p60·hsp70 Foldosome Complex J. Biol. Chem., March 27, 1998; 273(13): 7358 - 7366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Fliss, Y. Fang, F. Boschelli, and A. J. Caplan Differential In Vivo Regulation of Steroid Hormone Receptor Activation by Cdc37p Mol. Biol. Cell, December 1, 1997; 8(12): 2501 - 2509. [Abstract] [Full Text]
|
|
|
|
|
|
J. Rao, P. Lee, S. Benzeno, C. Cardozo, J. Albertus, D. M. Robins, and A. J. Caplan Functional Interaction of Human Cdc37 with the Androgen Receptor but Not with the Glucocorticoid Receptor J. Biol. Chem., February 16, 2001; 276(8): 5814 - 5820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Knee, B. A. Froesch, U. Nuber, S. Takayama, and J. C. Reed Structure-Function Analysis of Bag1 Proteins. EFFECTS ON ANDROGEN RECEPTOR TRANSCRIPTIONAL ACTIVITY J. Biol. Chem., April 13, 2001; 276(16): 12718 - 12724. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
