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Volume 271, Number 46, Issue of November 15, 1996 pp. 29255-29264
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Mouse RAD50 Has Limited Epitopic Homology to p53 and Is Expressed in the Adult Myocardium

(Received for publication, May 8, 1996, and in revised form, August 20, 1996)

Kyung Keun Kim , Adil I. Daud , Susan C. Wong , Laura Pajak , Shih-Chong Tsai , He Wang , William J. Henzel and Loren J. Field

From the  Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana 46202-4800 and the  Department of Protein Chemistry, Genentech Incorporated, South San Francisco, California 94080-4990

Previous studies have identified a 180-kDa mouse cardiomyocyte phosphoprotein with limited epitopic homology to p53. In this study, the protein was purified and partially sequenced. Oligonucleotide probes based on the available amino acid sequence data were used to isolate cDNA clones. Sequence analyses revealed that the clones encoded a protein with regional homology to the yeast RAD50 gene product. Expression of the mouse cDNA rescued the methyl methanesulfonate-sensitive phenotype in rad50 mutant yeast, indicating that the cardiomyocyte phosphoprotein is the mammalian homologue of the yeast RAD50 gene product. Fluorescence in situ hybridization analyses localized the mouse RAD50 gene to the A5-B1 region of chromosome 11. Northern blot analyses demonstrated a complex pattern of RAD50 expression during mouse development which was further complicated by the presence of several alternatively spliced transcripts. High levels of RAD50 expression was evident in the adult myocardium, a somewhat surprising observation given the absence of DNA synthesis in adult cardiomyocytes.

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