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Volume 271, Number 46, Issue of November 15, 1996 pp. 29386-29392
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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An ATPase Component of the Transcription Elongation Complex Is Required for Factor-dependent Transcription Termination by Vaccinia RNA Polymerase

(Received for publication, July 22, 1996, and in revised form, September 3, 1996)

From the Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021

Vaccinia virus RNA polymerase terminates transcription in response to a specific signal UUUUUNU in the nascent transcript. Transduction of this signal to the elongating polymerase requires a virus-encoded termination factor, VTF. The existence of a second termination factor was suggested by the finding that transient exposure of purified elongation complexes to heparin rendered them refractory to VTF-induced termination. Loss of termination competence correlated with the removal of several polypeptide components of the elongation complex. We present the identification of factor X, an activity that restored VTF responsiveness to heparin-stripped ternary complexes. We propose that factor X, which has an associated DNA-dependent ATPase activity, mediates the requirement for ATP hydrolysis during transcription termination.

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