|
|
|
|||||||
|
|||||||||
(Received for publication, June 6, 1996, and in revised form, July 29, 1996)
From the Institute of Interdisciplinary Research, Université
Libre de Bruxelles, Campus Erasme, 1070 Brussels, Belgium
Despite the similarity of their receptors and
signal transduction pathways, insulin is regarded as a regulator of
glucose, protein, and lipid metabolism, whereas insulin-like growth
factors (IGF-I and IGF-II) mainly act as mitogenic hormones. In the dog thyroid primary culture model, the triggering of DNA synthesis by
thyrotropin (TSH) through cAMP, or by cAMP-independent factors including epidermal growth factor, hepatocyte growth factor and phorbol
esters, requires insulin or IGFs as comitogenic factors. In the present
study, in TSH-treated cells, IGF-I receptors and insulin receptors were
paradoxically equivalent in their capacity to elicit the comitogenic
pathway, which, however, was mediated only by IGF-I receptors in dog
thyroid cells stimulated by cAMP-independent mitogens. Moreover, prior
cell exposure to TSH or forskolin increased their responsiveness to
insulin, IGF-I, and IGF-II, as seen on DNA synthesis and activation of
a common insulin/IGF signaling pathway. To understand these
observations, binding characteristics and expression of insulin and
IGF-I receptors were examined. To analyze IGF-I receptor
characteristics, the unexpected interference of a huge presence of
IGF-binding proteins at the cell membrane was avoided using labeled
Long R3 IGF-I instead of IGF-I. Strikingly, TSH, through
cAMP, time-dependently induced insulin binding and insulin
receptor mRNA and protein accumulation without any effect on IGF-I
receptors. These findings constitute a first example of an induction of
insulin receptor gene expression by a cAMP-mediated hormone. In dog
thyroid cells, this allows low physiological insulin concentrations to
act as a comitogenic factor and might explain in part the enhanced
responsiveness to IGFs in response to TSH. This raises the possibility
that TSH-insulin interactions may play a role in the regulation of
thyroid growth and function in vivo.
Volume 271, Number 46,
Issue of November 15, 1996
pp. 29400-29406
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Chakravarty, A. A. Santillan, C. Galer, H. P. Adams, A. K. El-Naggar, S. A. Jasser, S. Mohsin, D. Mondal, G. L. Clayman, and J. N. Myers Phosphorylated Insulin Like Growth Factor-I Receptor Expression and Its Clinico-Pathological Significance in Histologic Subtypes of Human Thyroid Cancer Experimental Biology and Medicine, April 1, 2009; 234(4): 372 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Costa, M. Senou, F. Van Rode, J. Ruf, M. Capello, D. Dequanter, P. Lothaire, C. Dessy, J. E. Dumont, M.-C. Many, et al. Reciprocal Negative Regulation between Thyrotropin/3',5'-Cyclic Adenosine Monophosphate-Mediated Proliferation and Caveolin-1 Expression in Human and Murine Thyrocytes Mol. Endocrinol., April 1, 2007; 21(4): 921 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paternot, J. E. Dumont, and P. P. Roger Differential Utilization of Cyclin D1 and Cyclin D3 in the Distinct Mitogenic Stimulations by Growth Factors and TSH of Human Thyrocytes in Primary Culture Mol. Endocrinol., December 1, 2006; 20(12): 3279 - 3292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Costa, Y. Song, P. Macours, C. Massart, M. C. Many, S. Costagliola, J. E. Dumont, J. Van Sande, and V. Vanvooren Sphingolipid-Cholesterol Domains (Lipid Rafts) in Normal Human and Dog Thyroid Follicular Cells Are Not Involved in Thyrotropin Receptor Signaling Endocrinology, March 1, 2004; 145(3): 1464 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Goffard, L. Jin, H. Mircescu, P. Van Hummelen, C. Ledent, J.-E. Dumont, and B. Corvilain Gene Expression Profile in Thyroid of Transgenic Mice Overexpressing the Adenosine Receptor 2a Mol. Endocrinol., January 1, 2004; 18(1): 194 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Clement, S. Refetoff, B. Robaye, J. E. Dumont, and S. Schurmans Low TSH Requirement and Goiter in Transgenic Mice Overexpressing IGF-I and IGF-I Receptor in the Thyroid Gland Endocrinology, December 1, 2001; 142(12): 5131 - 5139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kimura, A. Van Keymeulen, J. Golstein, A. Fusco, J. E. Dumont, and P. P. Roger Regulation of Thyroid Cell Proliferation by TSH and Other Factors: A Critical Evaluation of in Vitro Models Endocr. Rev., October 1, 2001; 22(5): 631 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Krohn and R. Paschke Progress in Understanding the Etiology of Thyroid Autonomy J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3336 - 3345. [Full Text] [PDF]
|
|
|
|
|
|
A. Van Keymeulen, S. Deleu, J. Bartek, J. E. Dumont, and P. P. Roger Respective Roles of Carbamylcholine and Cyclic Adenosine Monophosphate in Their Synergistic Regulation of Cell Cycle in Thyroid Primary Cultures Endocrinology, March 1, 2001; 142(3): 1251 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Derwahl, M. Broecker, and Z. Kraiem Thyrotropin May Not Be the Dominant Growth Factor in Benign and Malignant Thyroid Tumors J. Clin. Endocrinol. Metab., March 1, 1999; 84(3): 829 - 834. [Full Text]
|
|
|
|
 |
|
 F. Depoortere, A. Van Keymeulen, J. Lukas, S. Costagliola, J. Bartkova, J. E. Dumont, J. Bartek, P. P. Roger, and S. Dremier A Requirement for Cyclin D3-Cyclin-dependent Kinase (cdk)-4 Assembly in the Cyclic Adenosine Monophosphate-dependent Proliferation of Thyrocytes J. Cell Biol., March 23, 1998; 140(6): 1427 - 1439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |