| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, March 14, 1996, and in revised form, September 6, 1996)
From the Each of the three known mammalian
90-kDa S6 kinase (pp90rsk) isoforms (RSK1, RSK2, and RSK3) was
expressed in transfected cells and further characterized. The kinase
activity (immunocomplex toward S6 peptide) of each isoform was
activated by in vivo growth factor (epidermal growth factor
(EGF)) stimulation; RSK1 was more responsive (10-15-fold)
versus RSK2 and RSK3 (2-4-fold). Pretreatment with PD98059
(MEK1 inhibitor) partially (80%) blocked EGF-mediated ERK1 activation
and had similar effects on EGF stimulation of each ribosomal S6 kinase
(RSK). Cotransfection with dominant-negative MEK1 inhibited activation
of each RSK; furthermore, the kinase activity of RSK1, RSK2, and RSK3
was markedly increased by cotransfection with constitutively active
MEK1. A specific association between mitogen-activated protein kinases
(MAPKs) (ERK1 and ERK2) and RSK isoforms was tested by MAPK
immunoblotting after immunoprecipitation of RSKs. ERK1 and ERK2 were
present in RSK3 (and to a lesser extent, RSK2) immunoprecipitates, but
were absent in RSK1 immunoprecipitates. Both dephosphorylated (from
quiescent cells) and phosphorylated (from stimulated cells) MAPKs were
associated with RSK2 and RSK3. Deletion mutants of RSK3 were
characterized: the C terminus (33 residues) was shown to be required
for association with MAPKs. The kinase activity of RSK1 or RSK2 was
enhanced by in vitro incubation with ERK1. In contrast,
RSK3 activity was not affected by exposure to ERK1. Furthermore, MAPKs
in RSK3 immunoprecipitates were phosphorylated by purified MEK1;
however, RSK3 kinase activity was unaffected. We conclude that 1) the
MEK1-MAPK signaling pathway is both necessary and sufficient for
in vivo growth factor-mediated activation of all three RSK
isoforms; 2) RSK isoforms differ with respect to growth factor
responsiveness and their physical association with MAPK; and 3)
formation of the MAPK·RSK complex is mediated by the RSK C
terminus.
Volume 271, Number 47,
Issue of November 22, 1996
pp. 29773-29779
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
and**
Department of Medicine, Beth Israel Hospital
and Harvard Medical School, Boston, Massachusetts 02215 and the
** Department of Molecular Endocrinology, Merck Research Laboratories,
Rahway, New Jersey 07065
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Lu, J.-i. Abe, J. Taunton, Y. Lu, T. Shishido, C. McClain, C. Yan, S. P. Xu, T. M. Spangenberg, and H. Xu Reactive Oxygen Species-Induced Activation of p90 Ribosomal S6 Kinase Prolongs Cardiac Repolarization Through Inhibiting Outward K+ Channel Activity Circ. Res., August 1, 2008; 103(3): 269 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pelosi, F. Marampon, B. M. Zani, S. Prudente, E. Perlas, V. Caputo, L. Cianetti, V. Berno, S. Narumiya, S. W. Kang, et al. ROCK2 and Its Alternatively Spliced Isoform ROCK2m Positively Control the Maturation of the Myogenic Program Mol. Cell. Biol., September 1, 2007; 27(17): 6163 - 6176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Godeny and P. P. Sayeski ERK1/2 regulates ANG II-dependent cell proliferation via cytoplasmic activation of RSK2 and nuclear activation of elk1 Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1308 - C1317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Polychronopoulos, M. Verykokakis, M. N. Yazicioglu, M. Sakarellos-Daitsiotis, M. H. Cobb, and G. Mavrothalassitis The Transcriptional ETS2 Repressor Factor Associates with Active and Inactive Erks through Distinct FXF Motifs J. Biol. Chem., September 1, 2006; 281(35): 25601 - 25611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Sheffler, W. K. Kroeze, B. G. Garcia, A. Y. Deutch, S. J. Hufeisen, P. Leahy, J. C. Bruning, and B. L. Roth p90 ribosomal S6 kinase 2 exerts a tonic brake on G protein-coupled receptor signaling PNAS, March 21, 2006; 103(12): 4717 - 4722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ray Protection of Epithelial Cells by Keratincoyte Growth Factor Signaling Proceedings of the ATS, October 1, 2005; 2(3): 221 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Cieslik, Y. Zhu, M. Shtivelband, and K. K. Wu Inhibition of p90 Ribosomal S6 Kinase-mediated CCAAT/Enhancer-binding Protein {beta} Activation and Cyclooxygenase-2 Expression by Salicylate J. Biol. Chem., May 6, 2005; 280(18): 18411 - 18417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Dummler, C. Hauge, J. Silber, H. G. Yntema, L. S. Kruse, B. Kofoed, B. A. Hemmings, D. R. Alessi, and M. Frodin Functional Characterization of Human RSK4, a New 90-kDa Ribosomal S6 Kinase, Reveals Constitutive Activation in Most Cell Types J. Biol. Chem., April 8, 2005; 280(14): 13304 - 13314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garcia-Silva and A. Aranda The Thyroid Hormone Receptor Is a Suppressor of ras-Mediated Transcription, Proliferation, and Transformation Mol. Cell. Biol., September 1, 2004; 24(17): 7514 - 7523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, X. Fang, S. M. Dunham, C. Prada, E. K. Stachowiak, and M. K. Stachowiak 90-kDa Ribosomal S6 Kinase Is a Direct Target for the Nuclear Fibroblast Growth Factor Receptor 1 (FGFR1): ROLE IN FGFR1 SIGNALING J. Biol. Chem., July 9, 2004; 279(28): 29325 - 29335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. I. Fernando and J. Wimalasena Estradiol Abrogates Apoptosis in Breast Cancer Cells through Inactivation of BAD: Ras-dependent Nongenomic Pathways Requiring Signaling through ERK and Akt Mol. Biol. Cell, July 1, 2004; 15(7): 3266 - 3284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Roux and J. Blenis ERK and p38 MAPK-Activated Protein Kinases: a Family of Protein Kinases with Diverse Biological Functions Microbiol. Mol. Biol. Rev., June 1, 2004; 68(2): 320 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Myers, L. B. Corson, J. Rossant, and J. C. Baker Characterization of Mouse Rsk4 as an Inhibitor of Fibroblast Growth Factor-RAS-Extracellular Signal-Regulated Kinase Signaling Mol. Cell. Biol., May 15, 2004; 24(10): 4255 - 4266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sinnett-Smith, E. Zhukova, N. Hsieh, X. Jiang, and E. Rozengurt Protein Kinase D Potentiates DNA Synthesis Induced by Gq-coupled Receptors by Increasing the Duration of ERK Signaling in Swiss 3T3 Cells J. Biol. Chem., April 16, 2004; 279(16): 16883 - 16893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujita, S. Sato, and T. Tsuruo Phosphorylation of p27Kip1 at Threonine 198 by p90 Ribosomal Protein S6 Kinases Promotes Its Binding to 14-3-3 and Cytoplasmic Localization J. Biol. Chem., December 5, 2003; 278(49): 49254 - 49260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Eisenmann, M. W. VanBrocklin, N. A. Staffend, S. M. Kitchen, and H.-M. Koo Mitogen-Activated Protein Kinase Pathway-Dependent Tumor-Specific Survival Signaling in Melanoma Cells through Inactivation of the Proapoptotic Protein Bad Cancer Res., December 1, 2003; 63(23): 8330 - 8337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Jorgensen, R. Holm, G. M. Maelandsmo, and V. A. Florenes Expression of Activated Extracellular Signal-Regulated Kinases 1/2 in Malignant Melanomas: Relationship with Clinical Outcome Clin. Cancer Res., November 1, 2003; 9(14): 5325 - 5331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Roux, S. A. Richards, and J. Blenis Phosphorylation of p90 Ribosomal S6 Kinase (RSK) Regulates Extracellular Signal-Regulated Kinase Docking and RSK Activity Mol. Cell. Biol., July 15, 2003; 23(14): 4796 - 4804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. El-Haschimi, S. D. Dufresne, M. F. Hirshman, J. S. Flier, L. J. Goodyear, and C. Bjorbaek Insulin Resistance and Lipodystrophy in Mice Lacking Ribosomal S6 Kinase 2 Diabetes, June 1, 2003; 52(6): 1340 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. He, W.-Y. Ma, G. Liu, Y. Zhang, A. M. Bode, and Z. Dong Arsenite-induced Phosphorylation of Histone H3 at Serine 10 Is Mediated by Akt1, Extracellular Signal-regulated Kinase 2, and p90 Ribosomal S6 Kinase 2 but Not Mitogen- and Stress-activated Protein Kinase 1 J. Biol. Chem., March 14, 2003; 278(12): 10588 - 10593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hayashi, T. Okada, N. Igarashi, T. Fujita, S. Jahangeer, and S.-i. Nakamura Identification and Characterization of RPK118, a Novel Sphingosine Kinase-1-binding Protein J. Biol. Chem., August 30, 2002; 277(36): 33319 - 33324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Versteeg, B. B. Sorensen, S. H. Slofstra, J. H. M. Van den Brande, J. C. Stam, P. M. P. van Bergen en Henegouwen, D. J. Richel, L. C. Petersen, and M. P. Peppelenbosch VIIa/Tissue Factor Interaction Results in a Tissue Factor Cytoplasmic Domain-independent Activation of Protein Synthesis, p70, and p90 S6 Kinase Phosphorylation J. Biol. Chem., July 19, 2002; 277(30): 27065 - 27072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lu, M. Guizzetti, and L. G. Costa Inorganic Lead Activates the Mitogen-Activated Protein Kinase Kinase-Mitogen-Activated Protein Kinase-p90RSK Signaling Pathway in Human Astrocytoma Cells via a Protein Kinase C-Dependent Mechanism J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 818 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Di Agostino, P. Rossi, R. Geremia, and C. Sette The MAPK pathway triggers activation of Nek2 during chromosome condensation in mouse spermatocytes Development, January 4, 2002; 129(7): 1715 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Merienne, S. Pannetier, A. Harel-Bellan, and P. Sassone-Corsi Mitogen-Regulated RSK2-CBP Interaction Controls Their Kinase and Acetylase Activities Mol. Cell. Biol., October 15, 2001; 21(20): 7089 - 7096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Hall, J. Cui, M. E. Bates, B. A. Stout, L. Koenderman, P. J. Coffer, and P. J. Bertics Transduction of a dominant-negative H-Ras into human eosinophils attenuates extracellular signal-regulated kinase activation and interleukin-5-mediated cell viability Blood, October 1, 2001; 98(7): 2014 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
|
|
G.-Y. Wu, K. Deisseroth, and R. W. Tsien Activity-dependent CREB phosphorylation: Convergence of a fast, sensitive calmodulin kinase pathway and a slow, less sensitive mitogen-activated protein kinase pathway PNAS, February 15, 2001; (2001) 51634198. [Abstract] [Full Text]
|
|
|
|
|
|
S. D. Dufresne, C. Bjørbæk, K. El-Haschimi, Y. Zhao, W. G. Aschenbach, D. E. Moller, and L. J. Goodyear Altered Extracellular Signal-Regulated Kinase Signaling and Glycogen Metabolism in Skeletal Muscle from p90 Ribosomal S6 Kinase 2 Knockout Mice Mol. Cell. Biol., January 1, 2001; 21(1): 81 - 87. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Aoyagi and S. Izumo Hemodynamic Overload-Induced Activation of Myocardial Mitogen-Activated Protein Kinases In Vivo : Augmented Responses in Young Spontaneously Hypertensive Rats and Diminished Responses in Aged Fischer 344 Rats Hypertension, January 1, 2001; 37(1): 52 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Krook, U. Widegren, X. J. Jiang, J. Henriksson, H. Wallberg-Henriksson, D. Alessi, and J. R. Zierath Effects of exercise on mitogen- and stress-activated kinase signal transduction in human skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1716 - R1721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Stewart and K.-L. Guan The Dominant Negative Ras Mutant, N17Ras, Can Inhibit Signaling Independently of Blocking Ras Activation J. Biol. Chem., March 17, 2000; 275(12): 8854 - 8862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Ryder, R. Fahlman, H. Wallberg-Henriksson, D. R. Alessi, A. Krook, and J. R. Zierath Effect of Contraction on Mitogen-activated Protein Kinase Signal Transduction in Skeletal Muscle. INVOLVEMENT OF THE MITOGEN- AND STRESS-ACTIVATED PROTEIN KINASE 1 J. Biol. Chem., January 14, 2000; 275(2): 1457 - 1462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Schaefer, H. Kamiguchi, E. V. Wong, C. M. Beach, G. Landreth, and V. Lemmon Activation of the MAPK Signal Cascade by the Neural Cell Adhesion Molecule L1 Requires L1 Internalization J. Biol. Chem., December 31, 1999; 274(53): 37965 - 37973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shirakata, K. Ishii, H. Yagita, K. Okumura, M. Taniguchi, and T. Takemori Distinct Subcellular Localization and Substrate Specificity of Extracellular Signal-Regulated Kinase in B Cells upon Stimulation with IgM and CD40 J. Immunol., December 15, 1999; 163(12): 6589 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Stevenson, M.-J. Zhao, A. Asea, C. N. Coleman, and S. K. Calderwood Salicylic Acid and Aspirin Inhibit the Activity of RSK2 Kinase and Repress RSK2-Dependent Transcription of Cyclic AMP Response Element Binding Protein- and NF-{kappa}B-Responsive Genes J. Immunol., November 15, 1999; 163(10): 5608 - 5616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Lian, R. Huang, D. Robinson, and J. A. Badwey Activation of p90RSK and cAMP Response Element Binding Protein in Stimulated Neutrophils: Novel Effects of the Pyridinyl Imidazole SB 203580 on Activation of the Extracellular Signal-Regulated Kinase Cascade J. Immunol., October 15, 1999; 163(8): 4527 - 4536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Campbell, R. L. Sutherland, and R. J. Daly Signaling Pathways and Structural Domains Required for Phosphorylation of EMS1/Cortactin Cancer Res., October 1, 1999; 59(20): 5376 - 5385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Jensen, M.-B. Buch, T. O. Krag, B. A. Hemmings, S. Gammeltoft, and M. Frodin 90-kDa Ribosomal S6 Kinase Is Phosphorylated and Activated by 3-Phosphoinositide-dependent Protein Kinase-1 J. Biol. Chem., September 17, 1999; 274(38): 27168 - 27176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-C. Gavin, A. N. Ainle, E. Chierici, M. Jones, and A. R. Nebreda A p90rsk Mutant Constitutively Interacting with MAP Kinase Uncouples MAP Kinase from p34cdc2/Cyclin B Activation in Xenopus Oocytes Mol. Biol. Cell, September 1, 1999; 10(9): 2971 - 2986. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Trushin, K. N. Pennington, A. Algeciras-Schimnich, and C. V. Paya Protein Kinase C and Calcineurin Synergize to Activate Ikappa B Kinase and NF-kappa B in T Lymphocytes J. Biol. Chem., August 13, 1999; 274(33): 22923 - 22931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sassone-Corsi, C. A. Mizzen, P. Cheung, C. Crosio, L. Monaco, S. Jacquot, A. Hanauer, and C. D. Allis Requirement of Rsk-2 for Epidermal Growth Factor-Activated Phosphorylation of Histone H3 Science, August 6, 1999; 285(5429): 886 - 891. [Abstract] [Full Text]
|
|
|
|
 |
|
 O. M. Seternes, B. Johansen, and U. Moens A Dominant Role for the Raf-MEK Pathway in Forskolin, 12-O-Tetradecanoyl-phorbol Acetate, and Platelet-Derived Growth Factor-Induced CREB (cAMP-Responsive Element-Binding Protein) Activation, Uncoupled from Serine 133 Phosphorylation in NIH 3T3 Cells Mol. Endocrinol., July 1, 1999; 13(7): 1071 - 1083. [Abstract] [Full Text]
|
|
|
|
|
|
P. D. Adams, X. Li, W. R. Sellers, K. B. Baker, X. Leng, J. W. Harper, Y. Taya, and W. G. Kaelin Jr. Retinoblastoma Protein Contains a C-terminal Motif That Targets It for Phosphorylation by Cyclin-cdk Complexes Mol. Cell. Biol., February 1, 1999; 19(2): 1068 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Smith, C. E. Poteet-Smith, K. Malarkey, and T. W. Sturgill Identification of an Extracellular Signal-regulated Kinase (ERK) Docking Site in Ribosomal S6 Kinase, a Sequence Critical for Activation by ERK in Vivo J. Biol. Chem., January 29, 1999; 274(5): 2893 - 2898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W.-m. Lee Synergistic Activation of Mitogen-Activated Protein Kinase by Cyclic AMP and Myeloid Growth Factors Opposes Cyclic AMP's Growth-Inhibitory Effects Blood, January 15, 1999; 93(2): 537 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. New, M. Zhao, Y. Li, W. W. Bassett, Y. Feng, S. Ludwig, F. D. Padova, H. Gram, and J. Han Cloning and Characterization of RLPK, a Novel RSK-related Protein Kinase J. Biol. Chem., January 8, 1999; 274(2): 1026 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Angenstein, W. T. Greenough, and I. J. Weiler Metabotropic glutamate receptor-initiated translocation of protein kinase p90rsk to polyribosomes: A possible factor regulating synaptic protein synthesis PNAS, December 8, 1998; 95(25): 15078 - 15083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hodge, J. Liao, M. Stofega, K. Guan, C. Carter-Su, and J. Schwartz Growth Hormone Stimulates Phosphorylation and Activation of Elk-1 and Expression of c-fos, egr-1, and junB through Activation of Extracellular Signal-regulated Kinases 1 and 2 J. Biol. Chem., November 20, 1998; 273(47): 31327 - 31336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Pierrat, J. d. S. Correia, J.-L. Mary, M. Tomas-Zuber, and W. Lesslauer RSK-B, a Novel Ribosomal S6 Kinase Family Member, Is a CREB Kinase under Dominant Control of p38alpha Mitogen-activated Protein Kinase (p38alpha MAPK) J. Biol. Chem., November 6, 1998; 273(45): 29661 - 29671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. De Cesare, S. Jacquot, A. Hanauer, and P. Sassone-Corsi Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene PNAS, October 13, 1998; 95(21): 12202 - 12207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Beitner-Johnson and D. E. Millhorn Hypoxia Induces Phosphorylation of the Cyclic AMP Response Element-binding Protein by a Novel Signaling Mechanism J. Biol. Chem., July 31, 1998; 273(31): 19834 - 19839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xing, J. M. Kornhauser, Z. Xia, E. A. Thiele, and M. E. Greenberg Nerve Growth Factor Activates Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Pathways To Stimulate CREB Serine 133 Phosphorylation Mol. Cell. Biol., April 1, 1998; 18(4): 1946 - 1955. [Abstract] [Full Text]
|
|
|
|
|
|
K. N. Dalby, N. Morrice, F. B. Caudwell, J. Avruch, and P. Cohen Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK J. Biol. Chem., January 16, 1998; 273(3): 1496 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Butscher, C. Powers, M. Olive, C. Vinson, and K. Gardner Coordinate Transactivation of the Interleukin-2 CD28 Response Element by c-Rel and ATF-1/CREB2 J. Biol. Chem., January 2, 1998; 273(1): 552 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
