|
|
|
|||||||
|
|||||||||
(Received for publication, March 11, 1996, and in revised form, August 5, 1996)
From the Cat-1 is a protein with a dual function, a high
affinity, low capacity cationic amino acid transporter of the
y+ system and the receptor for the ecotropic retrovirus. We
have suggested that Cat-1 is required in the regenerating liver for the
transport of cationic amino acids and polyamines in the late G1 phase, a process that is essential for liver cells to
enter mitosis. In our earlier studies we had shown that the
cat-1 gene is silent in the quiescent liver but is induced
in response to hormones, insulin, and glucocorticoids, and partial
hepatectomy. Here we demonstrate that cat-1 is a classic
delayed early growth response gene in the regenerating liver, since
induction of its expression is sensitive to cycloheximide, indicating
that protein synthesis is required. The peak of accumulation of the
cat-1 mRNA (9-fold) by 3 h was not associated with
increased transcriptional activity of the cat-1 gene in the
regenerating liver, indicating post-transcriptional regulation of
expression of this gene. Induction of the cat-1 gene
results in the accumulation of two mRNA species (7.9 and 3.4 kilobase pairs (kb)). Both mRNAs hybridize with the previously
described rat cat-1/2.9-kb cDNA clone. However, the 3
Volume 271, Number 47,
Issue of November 22, 1996
pp. 29799-29806
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
,
,
Department of Nutrition, Case Western
Reserve University School of Medicine, Cleveland, Ohio 44106 and the
§ Departments of Pediatrics and Cell Biology, Baylor College
of Medicine, Houston, Texas 77030
end of a longer rat cat-1 cDNA (rat
cat-1/6.5-kb) hybridizes only to the 7.9-kb mRNA
transcript. Sequence analysis of this clone indicated that the two
mRNA species result from the use of alternative polyadenylation
signals. The 6.5-kb clone contains a number of AT-rich mRNA
destabilizing sequences which is reflected in the half-life of the
cat-1 mRNAs (90 min for 7.9-kb mRNA and 250 min for
3.4-kb mRNA). Treatment of rats with cycloheximide superinduces the
level of the 7.9-kb cat-1 mRNA in the kidney, spleen,
and brain, but not in the liver, suggesting that cell type-specific
labile factors are involved in its regulation. We conclude that the
need for protein synthesis for induction of the cat-1
mRNA, the short lived nature of the mRNAs, and the multiple sites for regulation of gene expression indicate a tight control of
expression of the cat-1 gene within the regenerating liver and suggest that y+ cationic amino acid transport in liver
cells is regulated at the molecular level.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. C. Rodriguez, D. G. Quiceno, and A. C. Ochoa L-arginine availability regulates T-lymphocyte cell-cycle progression Blood, February 15, 2007; 109(4): 1568 - 1573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shima, T. Maeda, S. Aizawa, I. Tsuboi, D. Kobayashi, R. Kato, and I. Tamai L-arginine import via cationic amino acid transporter CAT1 is essential for both differentiation and proliferation of erythrocytes Blood, February 15, 2006; 107(4): 1352 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gu, C. J. Villegas, and J. X. Jiang Differential Regulation of Amino Acid Transporter SNAT3 by Insulin in Hepatocytes J. Biol. Chem., July 15, 2005; 280(28): 26055 - 26062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Verri, C. Dimitri, S. Treglia, F. Storelli, S. De Micheli, L. Ulianich, P. Vito, S. Marsigliante, C. Storelli, and B. Di Jeso Multiple pathways for cationic amino acid transport in rat thyroid epithelial cell line PC Cl3 Am J Physiol Cell Physiol, February 1, 2005; 288(2): C290 - C303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rotmann, D. Strand, U. Martine, and E. I. Closs Protein Kinase C Activation Promotes the Internalization of the Human Cationic Amino Acid Transporter hCAT-1: A NEW REGULATORY MECHANISM FOR hCAT-1 ACTIVITY J. Biol. Chem., December 24, 2004; 279(52): 54185 - 54192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez, A. B. Lopez, C. Wang, R. Mishra, L. Zhou, I. Yaman, M. D. Snider, and M. Hatzolgou Transcriptional Control of the Arginine/Lysine Transporter, Cat-1, by Physiological Stress J. Biol. Chem., December 12, 2003; 278(50): 50000 - 50009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Yaman, J. Fernandez, B. Sarkar, R. J. Schneider, M. D. Snider, L. E. Nagy, and M. Hatzoglou Nutritional Control of mRNA Stability Is Mediated by a Conserved AU-rich Element That Binds the Cytoplasmic Shuttling Protein HuR J. Biol. Chem., October 25, 2002; 277(44): 41539 - 41546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vega-Agapito, A. Almeida, M. Hatzoglou, and J. P. Bolanos Peroxynitrite Stimulates L-Arginine Transport System y+ in Glial Cells. A POTENTIAL MECHANISM FOR REPLENISHING NEURONAL L-ARGININE J. Biol. Chem., August 9, 2002; 277(33): 29753 - 29759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Rodriguez, A. H. Zea, K. S. Culotta, J. Zabaleta, J. B. Ochoa, and A. C. Ochoa Regulation of T Cell Receptor CD3zeta Chain Expression by L-Arginine J. Biol. Chem., June 7, 2002; 277(24): 21123 - 21129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez, B. Bode, A. Koromilas, J. A. Diehl, I. Krukovets, M. D. Snider, and M. Hatzoglou Translation Mediated by the Internal Ribosome Entry Site of the cat-1 mRNA Is Regulated by Glucose Availability in a PERK Kinase-dependent Manner J. Biol. Chem., March 29, 2002; 277(14): 11780 - 11787. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takase-Yoden and R. Watanabe Distribution of ecotropic retrovirus receptor protein in rat brains detected by immunohistochemistry J. Gen. Virol., August 1, 2001; 82(8): 1815 - 1820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Easson, T. M. Pawlik, C. P. Fischer, J. L. Conroy, D. Sgroi, W. W. Souba, and B. P. Bode Tumor-influenced amino acid transport activities in zonal-enriched hepatocyte populations Am J Physiol Gastrointest Liver Physiol, December 1, 2000; 279(6): G1209 - G1218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wu, B. Cholewa, and D. L. Mattson Characterization of L-arginine transporters in rat renal inner medullary collecting duct Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2000; 278(6): R1506 - R1512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Aulak, R. Mishra, L. Zhou, S. L. Hyatt, W. de Jonge, W. Lamers, M. Snider, and M. Hatzoglou Post-transcriptional Regulation of the Arginine Transporter Cat-1 by Amino Acid Availability J. Biol. Chem., October 22, 1999; 274(43): 30424 - 30432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hattori, K. Kasai, and S. S. Gross Cationic amino acid transporter gene expression in cultured vascular smooth muscle cells and in rats Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H2020 - H2028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takase-Yoden and R. Watanabe Contribution of Virus-Receptor Interaction to Distinct Viral Proliferation of Neuropathogenic and Nonneuropathogenic Murine Leukemia Viruses in Rat Glial Cells J. Virol., May 1, 1999; 73(5): 4461 - 4464. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. PALACIN, R. ESTEVEZ, J. BERTRAN, and A. ZORZANO Molecular Biology of Mammalian Plasma Membrane Amino Acid Transporters Physiol Rev, October 1, 1998; 78(4): 969 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. DEVES and C. A. R. BOYD Transporters for Cationic Amino Acids in Animal Cells: Discovery, Structure, and Function Physiol Rev, April 1, 1998; 78(2): 487 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ito and M. Groudine A New Member of the Cationic Amino Acid Transporter Family Is Preferentially Expressed in Adult Mouse Brain J. Biol. Chem., October 17, 1997; 272(42): 26780 - 26786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Hyatt, K. S. Aulak, M. Malandro, M. S. Kilberg, and M. Hatzoglou Adaptive Regulation of the Cationic Amino Acid Transporter-1 (Cat-1) in Fao Cells J. Biol. Chem., August 8, 1997; 272(32): 19951 - 19957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez, I. Yaman, R. Mishra, W. C. Merrick, M. D. Snider, W. H. Lamers, and M. Hatzoglou Internal Ribosome Entry Site-mediated Translation of a Mammalian mRNA Is Regulated by Amino Acid Availability J. Biol. Chem., April 6, 2001; 276(15): 12285 - 12291. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |