| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, July 1, 1996, and in revised form, September 5, 1996)
From the Department of Molecular, Cellular, and Developmental
Biology, University of California,
Santa Barbara, California 93106
We have characterized the effects of vinblastine
on the growing and shortening dynamics at opposite ends of individual
bovine brain microtubules at steady state in vitro by video
microscopy. Vinblastine exerted strikingly different effects on the
dynamics and polymer mass at the plus and minus ends of microtubules.
At concentrations between 0.1 and 0.4 µM, the drug
strongly depolymerized microtubules at minus ends, whereas it did not
significantly depolymerize microtubules at plus ends. Vinblastine
stabilized plus ends by suppressing the rate and extent of growth and
shortening, decreasing the catastrophe frequency, and increasing the
rescue frequency. In contrast, vinblastine destabilized minus ends by
increasing the catastrophe frequency and decreasing the rescue
frequency, whereas it had no effect on the rate or extent of growth or
shortening. Thus, vinblastine moderately increased the overall
dynamicity at minus ends while strongly suppressing dynamicity at plus
ends. Both the kinetic destabilization of microtubules at minus ends and the stabilization at plus ends may contribute to the altered function of mitotic spindle microtubules of cells blocked in mitosis by
low concentrations of vinblastine.
Volume 271, Number 47,
Issue of November 22, 1996
pp. 29807-29812
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  O. Azarenko, T. Okouneva, K. W. Singletary, M. A. Jordan, and L. Wilson Suppression of microtubule dynamic instability and turnover in MCF7 breast cancer cells by sulforaphane Carcinogenesis, December 1, 2008; 29(12): 2360 - 2368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Galmarini, I. Treilleux, F. Cardoso, C. Bernard-Marty, V. Durbecq, D. Gancberg, M.-C. Bissery, M. Paesmans, D. Larsimont, M. J. Piccart, et al. Class III {beta}-Tubulin Isotype Predicts Response in Advanced Breast Cancer Patients Randomly Treated Either with Single-Agent Doxorubicin or Docetaxel Clin. Cancer Res., July 15, 2008; 14(14): 4511 - 4516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ray, T. Okouneva, T. Manna, H. P. Miller, S. Schmid, L. Arthaud, R. Luduena, M. A. Jordan, and L. Wilson Mechanism of Action of the Microtubule-Targeted Antimitotic Depsipeptide Tasidotin (Formerly ILX651) and Its Major Metabolite Tasidotin C-Carboxylate Cancer Res., April 15, 2007; 67(8): 3767 - 3776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Manna, D. Thrower, H. P. Miller, P. Curmi, and L. Wilson Stathmin Strongly Increases the Minus End Catastrophe Frequency and Induces Rapid Treadmilling of Bovine Brain Microtubules at Steady State in Vitro J. Biol. Chem., January 27, 2006; 281(4): 2071 - 2078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Jordan, K. Kamath, T. Manna, T. Okouneva, H. P. Miller, C. Davis, B. A. Littlefield, and L. Wilson The primary antimitotic mechanism of action of the synthetic halichondrin E7389 is suppression of microtubule growth Mol. Cancer Ther., July 1, 2005; 4(7): 1086 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Mussali-Galante, M. Avila-Costa, G Pinon-Zarate, G Martinez-Levy, V Rodriguez-Lara, M Rojas-Lemus, M. Avila-Casado, and T. Fortoul DNA damage as an early biomarker of effect in human health Toxicology and Industrial Health, June 1, 2005; 21(5-6): 155 - 166. [Abstract] [PDF]
|
|
|
|
|
|
T. Okouneva, B. T. Hill, L. Wilson, and M. A. Jordan The Effects of Vinflunine, Vinorelbine, and Vinblastine on Centromere Dynamics Mol. Cancer Ther., May 1, 2003; 2(5): 427 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Madari, D. Panda, L. Wilson, and R. S. Jacobs Dicoumarol: A Unique Microtubule Stabilizing Natural Product that Is Synergistic with Taxol Cancer Res., March 15, 2003; 63(6): 1214 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Newton, J. G. DeLuca, R. H. Himes, H. P. Miller, M. A. Jordan, and L. Wilson Intrinsically Slow Dynamic Instability of HeLa Cell Microtubules in Vitro J. Biol. Chem., November 1, 2002; 277(45): 42456 - 42462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Ngan, K. Bellman, D. Panda, B. T. Hill, M. A. Jordan, and L. Wilson Novel Actions of the Antitumor Drugs Vinflunine and Vinorelbine on Microtubules Cancer Res., September 1, 2000; 60(18): 5045 - 5051. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. G. Lloyd and C. D. Hardin Role of microtubules in the regulation of metabolism in isolated cerebral microvessels Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1250 - C1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Panda, H. P. Miller, and L. Wilson Rapid treadmilling of brain microtubules free of microtubule-associated proteins in vitro and its suppression by tau PNAS, October 26, 1999; 96(22): 12459 - 12464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lobert, J. W. Ingram, and J. J. Correia Additivity of Dilantin and Vinblastine Inhibitory Effects on Microtubule Assembly Cancer Res., October 1, 1999; 59(19): 4816 - 4822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Roychowdhury, D. Panda, L. Wilson, and M. M. Rasenick G Protein alpha Subunits Activate Tubulin GTPase and Modulate Microtubule Polymerization Dynamics J. Biol. Chem., May 7, 1999; 274(19): 13485 - 13490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Panda, K. DeLuca, D. Williams, M. A. Jordan, and L. Wilson Antiproliferative mechanism of action of cryptophycin-52: Kinetic stabilization of microtubule dynamics by high-affinity binding to microtubule ends PNAS, August 4, 1998; 95(16): 9313 - 9318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Panda, H. P. Miller, K. Islam, and L. Wilson Stabilization of microtubule dynamics by estramustine by binding to a novel site in tubulin: A possible mechanistic basis for its antitumor action PNAS, September 30, 1997; 94(20): 10560 - 10564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yvon and P Wadsworth Non-centrosomal microtubule formation and measurement of minus end microtubule dynamics in A498 cells J. Cell Sci., January 10, 1997; 110(19): 2391 - 2401. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
