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(Received for publication, July 1, 1996, and in revised form, September 5, 1996)
From the Department of Molecular, Cellular, and Developmental
Biology, University of California,
Santa Barbara, California 93106
We have characterized the effects of vinblastine
on the growing and shortening dynamics at opposite ends of individual
bovine brain microtubules at steady state in vitro by video
microscopy. Vinblastine exerted strikingly different effects on the
dynamics and polymer mass at the plus and minus ends of microtubules.
At concentrations between 0.1 and 0.4 µM, the drug
strongly depolymerized microtubules at minus ends, whereas it did not
significantly depolymerize microtubules at plus ends. Vinblastine
stabilized plus ends by suppressing the rate and extent of growth and
shortening, decreasing the catastrophe frequency, and increasing the
rescue frequency. In contrast, vinblastine destabilized minus ends by
increasing the catastrophe frequency and decreasing the rescue
frequency, whereas it had no effect on the rate or extent of growth or
shortening. Thus, vinblastine moderately increased the overall
dynamicity at minus ends while strongly suppressing dynamicity at plus
ends. Both the kinetic destabilization of microtubules at minus ends and the stabilization at plus ends may contribute to the altered function of mitotic spindle microtubules of cells blocked in mitosis by
low concentrations of vinblastine.
Volume 271, Number 47,
Issue of November 22, 1996
pp. 29807-29812
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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