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(Received for publication, June 28, 1996, and in revised form, September 9, 1996)
From the The effects of arachidonic acid (20:4, n-6) and
other fatty acids on the expression of stearoyl-CoA desaturase gene 1 were investigated in fully differentiated 3T3-L1 adipocytes. Treatment of 3T3-L1 adipocytes with arachidonic acid resulted in a decrease in
stearoyl-CoA desaturase (Scd) enzyme activity and scd1
mRNA. Arachidonic acid did not alter the transcription of the
scd1 gene, whereas the half-life of the scd1
mRNA was reduced from 25.1 to 8.5 h. Blocking the conversion
of arachidonic acid to eicosanoids by pretreatment of the cells with
cyclooxygenase, lipoxygenase, or cytochrome P-450 epoxygenase
inhibitors did not reverse the inhibition caused by arachidonic acid,
indicating that eicosanoid synthesis is not necessary for the
repression of scd1 mRNA expression. Treatment of
adipocytes with linoleic (18:2, n-6) and linolenic (18:3, n-3) acids
also resulted in inhibition of scd1 mRNA accumulation. By contrast, oleic acid (18:1, n-9) and stearic acid (18:0) had no
effect on scd1 mRNA levels. Taken together, these
results suggest that polyunsaturated fatty acids repress the expression
of the scd1 gene in mature adipocytes by reducing the
stability of scd1 mRNA.
Volume 271, Number 47,
Issue of November 22, 1996
pp. 29854-29858
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
,¶
Department of Biochemistry,
§ Department of Horticulture, and ¶ Nutritional
Sciences, University of Wisconsin, Madison, Wisconsin 53706
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