HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koller, E. Articles by Ranscht, B. Search for Related Content PubMed PubMed Citation Articles by Koller, E. Articles by Ranscht, B. Social Bookmarking                      What's this?

Volume 271, Number 47, Issue of November 22, 1996 pp. 30061-30067
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Differential Targeting of T- and N-cadherin in Polarized Epithelial Cells

(Received for publication, June 19, 1996, and in revised form, August 19, 1996)

From The Burnham Institute, La Jolla Cancer Research Center, La Jolla, California 92307

To test whether glycosyl phosphatidylinositol-linked T-cadherin is a component of cell junctions like classical cadherins, we have examined its distribution and targeting in polarized epithelial cells. In vivo, T-cadherin was detected on the apical cell surface of the chick intestinal epithelium. In cultures of transfected Madin-Darby canine kidney cells, T-cadherin was also expressed apically, whereas classical N-cadherin resided basolaterally. Both cadherins were directly targeted to their respective membrane domains. Mutant proteins were expressed in Madin-Darby canine kidney cells to identify the regions responsible for differential cadherin localization. Ncyt, an N-cadherin cytoplasmic domain deletion mutant, was stably distributed basolaterally. This mutant was transported to both the apical and basolateral membrane compartments, followed by preferential removal from the apical surface. T-Ncyt, a T-cadherin mutant with the N-cadherin cytoplasmic domain deletion, was localized basolaterally, whereas N-T_GPI, a GPI-anchored N-cadherin mutant, resided at the apical domain. The T-cadherin carboxyl-terminal 76 amino acids contain the apical targeting signal and include the signal for GPI anchor attachment. Basolateral localization of N-cadherin is achieved through targeting signals in the cytoplasmic domain. Thus, GPI-linked T-cadherin is not a component of cell junctions, consistent with a function as a recognition rather than a cell adhesion molecule.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. A. Dames, E. Bang, D. Haussinger, T. Ahrens, J. Engel, and S. Grzesiek Insights into the Low Adhesive Capacity of Human T-cadherin from the NMR Structure of Its N-terminal Extracellular Domain J. Biol. Chem., August 22, 2008; 283(34): 23485 - 23495. [Abstract] [Full Text] [PDF]

				M. Philippova, D. Ivanov, M. B. Joshi, E. Kyriakakis, K. Rupp, T. Afonyushkin, V. Bochkov, P. Erne, and T. J. Resink Identification of Proteins Associating with Glycosylphosphatidylinositol- Anchored T-Cadherin on the Surface of Vascular Endothelial Cells: Role for Grp78/BiP in T-Cadherin-Dependent Cell Survival Mol. Cell. Biol., June 15, 2008; 28(12): 4004 - 4017. [Abstract] [Full Text] [PDF]

				C. M. Lewis, L. R. Cler, D.-W. Bu, S. Zochbauer-Muller, S. Milchgrub, E. Z. Naftalis, A. M. Leitch, J. D. Minna, and D. M. Euhus Promoter Hypermethylation in Benign Breast Epithelium in Relation to Predicted Breast Cancer Risk Clin. Cancer Res., January 1, 2005; 11(1): 166 - 172. [Abstract] [Full Text] [PDF]

				Z.-y. Huang, Y. Wu, N. Hedrick, and D. H. Gutmann T-Cadherin-Mediated Cell Growth Regulation Involves G2 Phase Arrest and Requires p21CIP1/WAF1 Expression Mol. Cell. Biol., January 15, 2003; 23(2): 566 - 578. [Abstract] [Full Text] [PDF]

				M. Goldberg, M. Wei, B. Tycko, I. Falikovich, and D. Warburton Identification and expression analysis of the human {micro}-protocadherin gene in fetal and adult kidneys Am J Physiol Renal Physiol, September 1, 2002; 283(3): F454 - F463. [Abstract] [Full Text] [PDF]

				P. Navarro, L. Ruco, and E. Dejana Differential Localization of VE- and N-Cadherins in Human Endothelial Cells: VE-Cadherin Competes with N-Cadherin for Junctional Localization J. Cell Biol., March 23, 1998; 140(6): 1475 - 1484. [Abstract] [Full Text] [PDF]

				H. Yamada, B. Fredette, K. Shitara, K. Hagihara, R. Miura, B. Ranscht, W. B. Stallcup, and Y. Yamaguchi The Brain Chondroitin Sulfate Proteoglycan Brevican Associates with Astrocytes Ensheathing Cerebellar Glomeruli and Inhibits Neurite Outgrowth from Granule Neurons J. Neurosci., October 15, 1997; 17(20): 7784 - 7795. [Abstract] [Full Text] [PDF]