|
Volume 271, Number 47,
Issue of November 22, 1996
pp. 30089-30095
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
Modulation of JunD·AP-1 DNA Binding Activity by
AP-1-associated Factor 1 (AF-1)
(Received for publication, May 29, 1996, and in revised form, September 10, 1996)
Ciaran
Powers
,
Henry
Krutzsch
and
Kevin
Gardner
From the Laboratory of Pathology, NCI, National Institutes of
Health, Bethesda, Maryland 20892
AP-1-associated factor 1 (AF-1), is a novel
protein complex that dramatically enhances the assembly of
JunD-containing dimers onto AP-1 consensus sites. We describe the
partial purification of AF-1 from nuclear extracts of the T-cell line
MLA 144 by ionic, hydrophobic and gel filtration chromatography. AF-1
is a DNA-binding protein composed of low molecular mass polypeptides of
7-17 kDa that exists in solution as a 34-kDa complex. JunD
interactions with DNA are accelerated in the presence of AF-1 through
the formation of a true tri-molecular complex with JunD dimers and DNA
that assembles much more rapidly on DNA than JunD alone. DNA binding analysis of AF-1 interaction with JunD·AP-1 and DNA shows that AF-1
increases the DNA binding affinity of JunD for AP-1 sites over
100-fold. DNA cleavage footprint analysis of isolated AF-1·JunD DNA
complexes shows that the ternary complex makes nearly twice as many
contacts with DNA than JunD dimers alone. AF-1 interacts readily, but
differentially with Jun homodimers and Jun·Fos heterodimers. These
findings distinguish AF-1 as a significant protein-specific modulator
of AP-1·JunD in T-cells.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
K. Chmura, X. Bai, M. Nakamura, P. Kandasamy, M. McGibney, K. Kuronuma, H. Mitsuzawa, D. R. Voelker, and E. D. Chan
Induction of IL-8 by Mycoplasma pneumoniae membrane in BEAS-2B cells
Am J Physiol Lung Cell Mol Physiol,
July 1, 2008;
295(1):
L220 - L230.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. L. Smith, I. Collins, G. V. R. Chandramouli, W. G. Butscher, E. Zaitseva, W. J. Freebern, C. M. Haggerty, V. Doseeva, and K. Gardner
Targeting Combinatorial Transcriptional Complex Assembly at Specific Modules within the Interleukin-2 Promoter by the Immunosuppressant SB203580
J. Biol. Chem.,
October 17, 2003;
278(42):
41034 - 41046.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Chaudhry, W. J. Freebern, J. L. Smith, W. G. Butscher, C. M. Haggerty, and K. Gardner
Cross-Regulation of T Cell Growth Factor Expression by p53 and the Tax Oncogene
J. Immunol.,
December 15, 2002;
169(12):
6767 - 6778.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
V. Poulaki, C. S. Mitsiades, A. M. Joussen, A. Lappas, B. Kirchhof, and N. Mitsiades
Constitutive Nuclear Factor-{kappa}B Activity Is Crucial for Human Retinoblastoma Cell Viability
Am. J. Pathol.,
December 1, 2002;
161(6):
2229 - 2240.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Mitsiades, C. S. Mitsiades, V. Poulaki, D. Chauhan, P. G. Richardson, T. Hideshima, N. C. Munshi, S. P. Treon, and K. C. Anderson
Apoptotic signaling induced by immunomodulatory thalidomide analogs in human multiple myeloma cells: therapeutic implications
Blood,
May 29, 2002;
99(12):
4525 - 4530.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Mitsiades, C. S. Mitsiades, V. Poulaki, D. Chauhan, P. G. Richardson, T. Hideshima, N. Munshi, S. P. Treon, and K. C. Anderson
Biologic sequelae of nuclear factor-kappa B blockade in multiple myeloma: therapeutic applications
Blood,
May 13, 2002;
99(11):
4079 - 4086.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Q. Li, K. Gardner, L. Zhang, B. Tsang, F. Bostick-Bruton, and E. Reed
Cisplatin Induction of ERCC-1 mRNA Expression in A2780/CP70 Human Ovarian Cancer Cells
J. Biol. Chem.,
September 4, 1998;
273(36):
23419 - 23425.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. G. Butscher, C. Powers, M. Olive, C. Vinson, and K. Gardner
Coordinate Transactivation of the Interleukin-2 CD28 Response Element by c-Rel and ATF-1/CREB2
J. Biol. Chem.,
January 2, 1998;
273(1):
552 - 560.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Yasui, W. Butscher, M. Cohen, N. Spriggs, R. Wersto, E. C. Kohn, L. Liotta, and K. Gardner
Selective Inhibition of Mitogen-induced Transactivation of the HIV Long Terminal Repeat by Carboxyamidotriazole. CALCIUM INFLUX BLOCKADE REPRESSES HIV-1 TRANSCRIPTIONAL ACTIVATION
J. Biol. Chem.,
November 7, 1997;
272(45):
28762 - 28770.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. Olive, D. Krylov, D. R. Echlin, K. Gardner, E. Taparowsky, and C. Vinson
A Dominant Negative to Activation Protein-1 (AP1) That Abolishes DNA Binding and Inhibits Oncogenesis
J. Biol. Chem.,
July 25, 1997;
272(30):
18586 - 18594.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. G. Butscher, C. M. Haggerty, S. Chaudhry, and K. Gardner
Targeting of p300 to the Interleukin-2 Promoter via CREB-Rel Cross-talk during Mitogen and Oncogenic Molecular Signaling in Activated T-cells
J. Biol. Chem.,
July 13, 2001;
276(29):
27647 - 27656.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. V. Kumar and L. R. Bernstein
Ten ERK-related Proteins in Three Distinct Classes Associate with AP-1 Proteins and/or AP-1 DNA
J. Biol. Chem.,
August 17, 2001;
276(34):
32362 - 32372.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|