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(Received for publication, August 23, 1996, and in revised form, September 30, 1996)
From the Departments of The serine/threonine protein kinase Raf-1 is
activated in response to a variety of growth factors in fibroblasts and
hematopoietic cells. In T cells, Raf-1 is activated in response to
stimulation through the T cell antigen receptor, the interleukin-2
receptor, and by stimulation of protein kinase C. We demonstrate here
that in T cells, Raf-1 is also activated during mitosis. The mitotic activation of Raf-1 was not observed in the Lck-deficient cell line,
J.CaM.1. During mitosis, Raf-1 was found to interact selectively with a
mitotic form of Lck that migrated with a reduced electrophoretic mobility on SDS-polyacrylamide gels. We conclude that Raf-1 is activated during mitosis in T cells and that this mitotic activation of
Raf-1 is dependent on the presence of Lck.
Volume 271, Number 48,
Issue of November 29, 1996
pp. 30315-30317
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
COMMUNICATION:
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Biology and ¶ Medicinal
Chemistry and Molecular Pharmacology, Purdue University, West
Lafayette, Indiana 47907
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