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Volume 271, Number 48, Issue of November 29, 1996 pp. 30672-30676
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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Cleavage of Insertion/Deletion Mismatches, Flap and Pseudo-Y DNA Structures by Deoxyinosine 3-Endonuclease from Escherichia coli

(Received for publication, September 3, 1996)

From the Division of Cancer Biology, Department of Radiation Oncology, Emory University School of Medicine, Atlanta, Georgia 30335

Deoxyinosine 3-endonuclease, an Escherichia coli repair enzyme that recognizes and cleaves DNA containing deoxyinosine and base mismatches, can cleave heteroduplexes containing a hairpin or unpaired loop. These DNA structures, referred to as insertion/deletion mismatches (IDM), are abnormal intermediate structures generated during replication of repetitive DNA sequences. In addition, the enzyme also cleaved the 5-single-stranded tails of flap and pseudo-Y DNA structures, suggesting that deoxyinosine 3-endonuclease is a bacterial functional homologue of human FEN1 and yeast RTH1 nucleases. These biochemical properties suggest that deoxyinosine 3-endonuclease might be important in the repair of IDM structures generated in lagging strand during DNA replication.

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