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(Received for publication, March 11, 1996, and in revised form, July 3, 1996)
From the Division of Molecular Genetics, Institute for Biomedical
and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United
Kingdom
Vacuolar ATPases play major roles in endomembrane
and plasma membrane proton transport in eukaryotes. A Drosophila
melanogaster cDNA encoding vha55, the 55-kDa
vacuolar ATPase (V-ATPase) regulatory B-subunit, was characterized and
mapped to 87C2-4 on chromosome 3R. A fly line was identified that
carried a single lethal P-element insertion within the coding portion
of gene, and its LacZ reporter gene revealed elevated
expression in Malpighian tubules, rectum, antennal palps, and oviduct,
regions where V-ATPases are believed to play a plasma membrane, rather
than an endomembrane, role. The P-element vha55 insertion
was shown to be allelic to a known lethal complementation group
l(3)SzA (= l(3)87Ca) at 87C, for which many
alleles have been described previously. Deletions of the locus have
been shown to be larval lethal, whereas point mutations show a range of
phenotypes from subvital to embryonic lethal, implying that severe
alleles confer a partial dominant negative phenotype. The P-element
null allele of vha55 was shown also to suppress ectopic sex
combs in Polycomb males, suggesting that transcriptional
silencing may be modulated by genes other than those with known
homeotic or DNA binding functions.
Volume 271, Number 48,
Issue of November 29, 1996
pp. 30677-30684
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
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