|
|
|
|||||||
|
|||||||||
(Received for publication, March 11, 1996, and in revised form, July 3, 1996)
From the Division of Molecular Genetics, Institute for Biomedical
and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United
Kingdom
Vacuolar ATPases play major roles in endomembrane
and plasma membrane proton transport in eukaryotes. A Drosophila
melanogaster cDNA encoding vha55, the 55-kDa
vacuolar ATPase (V-ATPase) regulatory B-subunit, was characterized and
mapped to 87C2-4 on chromosome 3R. A fly line was identified that
carried a single lethal P-element insertion within the coding portion
of gene, and its LacZ reporter gene revealed elevated
expression in Malpighian tubules, rectum, antennal palps, and oviduct,
regions where V-ATPases are believed to play a plasma membrane, rather
than an endomembrane, role. The P-element vha55 insertion
was shown to be allelic to a known lethal complementation group
l(3)SzA (= l(3)87Ca) at 87C, for which many
alleles have been described previously. Deletions of the locus have
been shown to be larval lethal, whereas point mutations show a range of
phenotypes from subvital to embryonic lethal, implying that severe
alleles confer a partial dominant negative phenotype. The P-element
null allele of vha55 was shown also to suppress ectopic sex
combs in Polycomb males, suggesting that transcriptional
silencing may be modulated by genes other than those with known
homeotic or DNA binding functions.
Volume 271, Number 48,
Issue of November 29, 1996
pp. 30677-30684
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Wieczorek, K. W. Beyenbach, M. Huss, and O. Vitavska Vacuolar-type proton pumps in insect epithelia J. Exp. Biol., June 1, 2009; 212(11): 1611 - 1619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. T. Dow Insights into the Malpighian tubule from functional genomics J. Exp. Biol., February 1, 2009; 212(3): 435 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Martinez-Munoz and P. Kane Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast J. Biol. Chem., July 18, 2008; 283(29): 20309 - 20319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Horng, L.-Y. Lin, C.-J. Huang, F. Katoh, T. Kaneko, and P.-P. Hwang Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R2068 - R2076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Rizzo, M. Tarsio, G. A. Martinez-Munoz, and P. M. Kane Diploids Heterozygous for a vma13{Delta} Mutation in Saccharomyces cerevisiae Highlight the Importance of V-ATPase Subunit Balance in Supporting Vacuolar Acidification and Silencing Cytosolic V1-ATPase Activity J. Biol. Chem., March 16, 2007; 282(11): 8521 - 8532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Du, L. Kean, A. K. Allan, T. D. Southall, S. A. Davies, C. J. McInerny, and J. A. T. Dow The SzA mutations of the B subunit of the Drosophila vacuolar H+ ATPase identify conserved residues essential for function in fly and yeast J. Cell Sci., June 15, 2006; 119(12): 2542 - 2551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway PLANT CELL, May 1, 2006; 18(5): 1274 - 1291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Kane The Where, When, and How of Organelle Acidification by the Yeast Vacuolar H+-ATPase Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 177 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Beyenbach and H. Wieczorek The V-type H+ ATPase: molecular structure and function, physiological roles and regulation J. Exp. Biol., February 15, 2006; 209(4): 577 - 589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Allan, J. Du, S. A. Davies, and J. A. T. Dow Genome-wide survey of V-ATPase genes in Drosophila reveals a conserved renal phenotype for lethal alleles Physiol Genomics, July 14, 2005; 22(2): 128 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Jung, B. Denholm, H. Skaer, and M. Affolter Renal Tubule Development in Drosophila: A Closer Look at the Cellular Level J. Am. Soc. Nephrol., February 1, 2005; 16(2): 322 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hilson, J. Allemeersch, T. Altmann, S. Aubourg, A. Avon, J. Beynon, R. P. Bhalerao, F. Bitton, M. Caboche, B. Cannoot, et al. Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications Genome Res., October 1, 2004; 14(10b): 2176 - 2189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. T. DOW and S. A. DAVIES Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism Physiol Rev, July 1, 2003; 83(3): 687 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zimmermann, P. Dames, B. Walz, and O. Baumann Distribution and serotonin-induced activation of vacuolar-type H+-ATPase in the salivary glands of the blowfly Calliphora vicina J. Exp. Biol., June 1, 2003; 206(11): 1867 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Liu, C. Mirschberger, L. Chooback, Q. Arana, Z. Dal Sacco, H. MacWilliams, and M. Clarke Altered expression of the 100 kDa subunit of the Dictyostelium vacuolar proton pump impairs enzyme assembly, endocytic function and cytosolic pH regulation J. Cell Sci., January 5, 2002; 115(9): 1907 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Miura, S. Miyazawa, S. Furuta, J. Mitsushita, K. Kamijo, H. Ishida, T. Miki, K. Suzukawa, J. Resau, T. D. Copeland, et al. The Sos1-Rac1 Signaling. POSSIBLE INVOLVEMENT OF A VACUOLAR H+-ATPase E SUBUNIT J. Biol. Chem., November 30, 2001; 276(49): 46276 - 46283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. MacPherson, V. P. Pollock, K. E. Broderick, L. Kean, F. C. O'Connell, J. A. T. Dow, and S. A. Davies Model Organisms: New Insights Into Ion Channel and Transporter Function.: L-type calcium channels regulate epithelial fluid transport in Drosophila melanogaster Am J Physiol Cell Physiol, February 1, 2001; 280(2): C394 - C407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Giannakou and J. A. T. Dow Characterization of the Drosophila melanogaster alkali-metal/proton exchanger (NHE) gene family J. Exp. Biol., January 11, 2001; 204(21): 3703 - 3716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Bowman, R. Kendle, and B. J. Bowman Disruption of vma-1, the Gene Encoding the Catalytic Subunit of the Vacuolar H+-ATPase, Causes Severe Morphological Changes in Neurospora crassa J. Biol. Chem., January 7, 2000; 275(1): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perry, M. Beyers, and D. Johnson Cloning and molecular characterisation of the trout (Oncorhynchus mykiss) vacuolar H(+)-ATPase B subunit J. Exp. Biol., January 2, 2000; 203(3): 459 - 470. [Abstract] [PDF]
|
|
|
|
|
|
E. Bowman and B. Bowman Cellular role of the V-ATPase in Neurospora crassa: analysis of mutants resistant to concanamycin or lacking the catalytic subunit A J. Exp. Biol., January 1, 2000; 203(1): 97 - 106. [Abstract]
|
|
|
|
|
|
M Futai, T Oka, G Sun-Wada, Y Moriyama, H Kanazawa, and Y Wada Luminal acidification of diverse organelles by V-ATPase in animal cells J. Exp. Biol., January 1, 2000; 203(1): 107 - 116. [Abstract]
|
|
|
|
 |
|
 K. Schumacher, D. Vafeados, M. McCarthy, H. Sze, T. Wilkins, and J. Chory The Arabidopsis det3 mutant reveals a central role for the vacuolar H+-ATPase in plant growth and development Genes & Dev., December 15, 1999; 13(24): 3259 - 3270. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. C. Spradling, D. Stern, A. Beaton, E. J. Rhem, T. Laverty, N. Mozden, S. Misra, and G. M. Rubin The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-Element Insertions Mutating 25% of Vital Drosophila Genes Genetics, September 1, 1999; 153(1): 135 - 177. [Abstract] [Full Text]
|
|
|
|
|
|
S. O. Cociancich, S. S. Park, D. A. Fidock, and M. Shahabuddin Vesicular ATPase-overexpressing Cells Determine the Distribution of Malaria Parasite Oocysts on the Midguts of Mosquitoes J. Biol. Chem., April 30, 1999; 274(18): 12650 - 12655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Terhzaz, F. O'Connell, V. Pollock, L Kean, S. Davies, J. Veenstra, and J. Dow Isolation and characterization of a leucokinin-like peptide of Drosophila melanogaster J. Exp. Biol., January 12, 1999; 202(24): 3667 - 3676. [Abstract] [PDF]
|
|
|
|
|
|
T. Oka, R. Yamamoto, and M. Futai Multiple Genes for Vacuolar-type ATPase Proteolipids in Caenorhabditis elegans. A NEW GENE, vha-3, HAS A DISTINCT CELL-SPECIFIC DISTRIBUTION J. Biol. Chem., August 28, 1998; 273(35): 22570 - 22576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Zhang, K. J. Parra, J. Liu, and P. M. Kane Characterization of a Temperature-sensitive Yeast Vacuolar ATPase Mutant with Defects in Actin Distribution and Bud Morphology J. Biol. Chem., July 17, 1998; 273(29): 18470 - 18480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, R. Yamamoto, and M. Futai Three vha Genes Encode Proteolipids of Caenorhabditis elegans Vacuolar-type ATPase. GENE STRUCTURES AND PREFERENTIAL EXPRESSION IN AN H-SHAPED EXCRETORY CELL AND RECTAL CELLS J. Biol. Chem., September 26, 1997; 272(39): 24387 - 24392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Sozen, J. D. Armstrong, M. Yang, K. Kaiser, and J. A. T. Dow Functional domains are specified to single-cell resolution in a Drosophila epithelium PNAS, May 13, 1997; 94(10): 5207 - 5212. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |