JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gao, P.
Right arrow Articles by Malbon, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, P.
Right arrow Articles by Malbon, C. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 271, Number 48, Issue of November 29, 1996 pp. 30692-30698
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

Differentiation of F9 Teratocarcinoma Stem Cells to Primitive Endoderm Is Regulated by the Gialpha 2/Gsalpha Axis via Phospholipase C and Not Adenylylcyclase

(Received for publication, July 19, 1996, and in revised form, September 10, 1996)

Ping Gao and Craig C. Malbon

From the Department of Molecular Pharmacology, Diabetes and Metabolic Diseases Research Center, School of Medicine-HSC, State University of New York, Stony Brook, New York 11794-8651

Morphogen-induced decline in Gialpha triggers F9 teratocarcinoma stem cells to progress to primitive endoderm via activation of protein kinase C and mitogen-activated protein kinase (Gao, P., and Malbon, C. C. (1996) J. Biol. Chem. 271, 9002-9008). Constitutive expression of Gialpha 2 blocks, whereas expression of Gsalpha provokes, progression to primitive endoderm, permitting identification of the effectors of the response-utilizing chimera created between Gialpha 2 and Gsalpha . N-terminal substitution of Gsalpha with Gialpha 2 sequence to create chimera Gialpha 2 (1-54)/Gsalpha produced a chimera that activated adenylylcyclase but abolished progression to primitive endoderm and activation of phospholipase C. C-terminal substitution of Gsalpha with Gialpha 2 sequence to Gsalpha /Gialpha 2 (320-355) enhanced the ability of Gsalpha to promote progression. The Q205L-activated mutant of Gialpha 2 suppresses, whereas the G225T-activated mutant of Gsalpha strongly activates phospholipase C and progression in these cells. The N-terminal region of Gsalpha (residues 62-86) appears to act as a dominant switch for the Gsalpha - (activation) versus Gialpha 2- (suppression) mediated control of phospholipase C and progression to primitive endoderm.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y.-N. Lee, C. C. Malbon, and H.-y. Wang
G{alpha}13 Signals via p115RhoGEF Cascades Regulating JNK1 and Primitive Endoderm Formation
J. Biol. Chem., December 24, 2004; 279(52): 54896 - 54904.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kihara, M. Ikeda, Y. Kariya, E.-Y. Lee, Y.-M. Lee, and Y. Igarashi
Sphingosine-1-phosphate Lyase Is Involved in the Differentiation of F9 Embryonal Carcinoma Cells to Primitive Endoderm
J. Biol. Chem., April 11, 2003; 278(16): 14578 - 14585.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Liu, Y.-N. Lee, C. C. Malbon, and H.-y. Wang
Activation of the beta -Catenin/Lef-Tcf Pathway Is Obligate for Formation of Primitive Endoderm by Mouse F9 Totipotent Teratocarcinoma Cells in Response to Retinoic Acid
J. Biol. Chem., August 16, 2002; 277(34): 30887 - 30891.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Tao, C. C. Malbon, and H.-y. Wang
Galpha i2 Enhances Insulin Signaling via Suppression of Protein-tyrosine Phosphatase 1B
J. Biol. Chem., October 19, 2001; 276(43): 39705 - 39712.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Liu, T. Liu, D. C. Slusarski, J. Yang-Snyder, C. C. Malbon, R. T. Moon, and H.-y. Wang
Activation of a Frizzled-2/beta -adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via Galpha o and Galpha t
PNAS, December 7, 1999; 96(25): 14383 - 14388.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Liu, X. Liu, H.-y. Wang, R. T. Moon, and C. C. Malbon
Activation of Rat Frizzled-1 Promotes Wnt Signaling and Differentiation of Mouse F9 Teratocarcinoma Cells via Pathways That Require Galpha q and Galpha o Function
J. Biol. Chem., November 19, 1999; 274(47): 33539 - 33544.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kanungo, I. Potapova, C. C. Malbon, and H.-y. Wang
MEKK4 Mediates Differentiation in Response to Retinoic Acid via Activation of c-Jun N-terminal Kinase in Rat Embryonal Carcinoma P19 Cells
J. Biol. Chem., July 28, 2000; 275(31): 24032 - 24039.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Yu, A. Castle, M. Chen, R. Lee, K. Takeda, and L. S. Weinstein
Increased Insulin Sensitivity in Gsalpha Knockout Mice
J. Biol. Chem., June 1, 2001; 276(23): 19994 - 19998.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1996 by the American Society for Biochemistry and Molecular Biology.