|
|
|
|||||||
|
|||||||||
(Received for publication, June 21, 1996, and in revised form, September 12, 1996)
From the Unité Mixte de Recherche 146 du CNRS, Institut
Curie, Laboratoire 110, Centre Universitaire,
91405 Orsay Cédex, France
We reported previously that post-mitotic chicken
embryonic neuroretina (NR) cells are induced to proliferate following
in vitro infection with RAV-1, a retrovirus that does not
carry an oncogene. NR cell multiplication results from the frequent
activation and subsequent retroviral transduction of two related
serine/threonine protein kinases, the
c-mil/c-raf or
c-Rmil/B-raf genes. We also showed that a very
early event in the activation of these proto-oncogenes is the synthesis
of chimeric mRNAs containing viral and cellular sequences joined by
a splicing mechanism. In the current study, we have examined the
ability of RAV-1 to induce proliferation of quail NR cells. By using
the reverse transcription-polymerase chain reaction technique, we
identified, in several proliferating quail NR cultures infected with
RAV-1, a chimeric mRNA containing cellular sequences joined to the
RAV-1 splice donor site. These cellular sequences are derived from a
gene designated R10, which is expressed through a
1.9-kilobase (kb) mRNA detected in several embryonic tissues. A
second transcript of 2.3 kb is specifically expressed in the NR, where
both transcripts are developmentally regulated. The R10 cDNA
encodes a 251-amino acid polypeptide that contains a leucine zipper
motif. It exhibits significant similarity with the putative D52/N8L
protein, encoded by an mRNA reported previously to be overexpressed
in human breast and lung carcinomas. By using polyclonal antibodies
specific for its amino-terminal and leucine zipper-containing regions,
we identified the R10 gene product as a cytoplasmic protein
of 23 kDa in cultured avian fibroblasts. A second protein of 30 kDa is
detected in post-mitotic NR cells that express the 2.3-kb transcript.
We also show, by in vitro transcription/translation and
immunoprecipitation, that the R10 protein can readily form homodimers,
presumably through its leucine zipper motif.
Volume 271, Number 48,
Issue of November 29, 1996
pp. 30790-30797
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. D. Lewis, L. A. Payton, J. G. Whitford, J. A. Byrne, D. I. Smith, L. Yang, and R. K. Bright Induction of Tumorigenesis and Metastasis by the Murine Orthologue of Tumor Protein D52 Mol. Cancer Res., February 1, 2007; 5(2): 133 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cho, H.-M. Ko, J.-M. Kim, J.-A Lee, J.-E. Park, M.-S. Jang, S. G. Park, D. H. Lee, S.-E. Ryu, and B.-C. Park Positive Regulation of Apoptosis Signal-regulating Kinase 1 by hD53L1 J. Biol. Chem., April 16, 2004; 279(16): 16050 - 16056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Wang, J. Xu, O. Saramaki, T. Visakorpi, W. M. Sutherland, J. Zhou, B. Sen, S. D. Lim, N. Mabjeesh, M. Amin, et al. PrLZ, a Novel Prostate-Specific and Androgen-Responsive Gene of the TPD52 Family, Amplified in Chromosome 8q21.1 and Overexpressed in Human Prostate Cancer Cancer Res., March 1, 2004; 64(5): 1589 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Groblewski, M. Yoshida, H. Yao, J. A. Williams, and S. A. Ernst Immunolocalization of CRHSP28 in exocrine digestive glands and gastrointestinal tissues of the rat Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G219 - G226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. H. Thomas, W. B. Taft, K. M. Kaspar, and G. E. Groblewski CRHSP-28 Regulates Ca2+-stimulated Secretion in Permeabilized Acinar Cells J. Biol. Chem., July 27, 2001; 276(31): 28866 - 28872. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |