HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Urban, R. J. Articles by Bodenburg, Y. Search for Related Content PubMed PubMed Citation Articles by Urban, R. J. Articles by Bodenburg, Y. Social Bookmarking                      What's this?

Volume 271, Number 49, Issue of December 6, 1996 pp. 31699-31703
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Tumor Necrosis Factor  Inhibits Transcriptional Activity of the Porcine P-45011A Insulin-like Growth Factor Response Element

(Received for publication, June 27, 1996)

Randall J. Urban , Manubai Nagamani ^¶ and Yvonne Bodenburg

From the  Division of Endocrinology, Department of Internal Medicine and the ^¶ Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas 77555-0587

We investigated the effects of tumor necrosis factor  (TNF) on the transcriptional activity of the porcine P-45011A (P450scc) insulin-like growth factor response element (IGFRE). TNF inhibited insulin-like growth factor-I (IGF-I)-stimulated P450scc mRNA concentrations in cultures of porcine granulosa cells. Transient transfection experiments in granulosa cells with deletion P450scc/luciferase constructs showed that TNF inhibited the transcriptional activity of the IGFRE. IGF-I binding and IGF-I receptor mRNA concentrations in porcine granulosa cells were not inhibited by TNF. Electrophoretic mobility shift assay with nuclear extract protein from porcine granulosa cells treated with IGF-I and TNF showed that Sp1 and a second transcription factor, P2, bound to the IGFRE. While IGF-I treatment increased the binding activity of both factors, TNF specifically inhibited the IGF-I-stimulated binding activity of P2. Transient transfection studies done in mouse fibroblasts overexpressing the IGF-I receptor (NWTb3) with the porcine IGFRE (three repeats) in an SV40/luciferase construct also showed TNF inhibited IGF-I-stimulated reporter gene expression. We conclude that TNF inhibits the transcriptional activity of the porcine P450scc IGFRE by preventing IGF-I-stimulated binding of P2.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Natesampillai, J. Kerkvliet, P. C. K. Leung, and J. D. Veldhuis Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E385 - E391. [Abstract] [Full Text] [PDF]

				R. J. Urban, Y. H. Bodenburg, J. Jiang, L. Denner, and J. Chedrese Protein kinase C{iota} enhances the transcriptional activity of the porcine P-450 side-chain cleavage insulin-like response element Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E975 - E979. [Abstract] [Full Text] [PDF]

				R. J. Urban and Y. Bodenburg PTB-associated splicing factor regulates growth factor-stimulated gene expression in mammalian cells Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E794 - E798. [Abstract] [Full Text] [PDF]

				R. J. Urban, Y. H. Bodenburg, and T. G. Wood NH2 terminus of PTB-associated splicing factor binds to the porcine P450scc IGF-I response element Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E423 - E427. [Abstract] [Full Text] [PDF]

				R. J. Urban, Y. Bodenburg, A. Kurosky, T. G. Wood, and S. Gasic Polypyrimidine Tract-Binding Protein-Associated Splicing Factor Is a Negative Regulator of Transcriptional Activity of the Porcine P450scc Insulin-Like Growth Factor Response Element Mol. Endocrinol., June 1, 2000; 14(6): 774 - 782. [Abstract] [Full Text]