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Volume 271, Number 51, Issue of December 20, 1996 pp. 32593-32598
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

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The Mechanism of GT Element-mediated Cell Type-specific Transcriptional Control

(Received for publication, June 24, 1996, and in revised form, September 4, 1996)

From the Laboratoire de Génétique Moléculaire des Plantes, Unité Mixte de Recherche-5575, Centre National de la Recherche Scientifique, Université de Grenoble 1, 38041 Grenoble cédex, France

Promoter studies have revealed that sequences related to the GT-1 binding site, known as GT elements, are conserved in plant nuclear genes of diverse functions. In this work, we addressed the issue of whether GT elements are involved in cell type-specific transcriptional regulation. We found that the inactivation of GT-1 site-mediated transcription in roots is correlated with the absence of the GT-1 binding activity in root extracts. In addition, the mutation of the related GT-1 (from the pea rbcs-3A) and the S1F (from the spinach rps1) sites resulted in an increase of their transcriptional activity in roots that contain a distinct GT element-binding factor, referred to as RGTF. Although specific to GT elements, RGTF has a different sequence requirement and a lower sequence specificity than GT-1. Interestingly, RGTF has a higher binding affinity to the mutant GT-1 and S1F sites than to the wild-type sequences. This correlation suggests that RGTF may have some role in transcriptional regulation in roots. Furthermore, root cellular protein extracts contain an inhibitory activity that prevents GT-1 from binding to DNA. This helps to explain the absence of the GT-1 binding activity in roots in which the gene of GT-1 is expressed. Together, these data suggest that the cell type-specific transcription modulation by GT elements is achieved by using two different strategies.

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