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Volume 271, Number 52, Issue of December 27, 1996 pp. 33261-33267
©1996 by The American Society for Biochemistry and Molecular Biology, Inc.

The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNA

(Received for publication, May 1, 1996, and in revised form, September 10, 1996)

Christine L. O'Day , Gloria Dalbadie-McFarland and John Abelson

From the Division of Biology, California Institute of Technology, Pasadena, California 91125

The Saccharomyces cerevisiae protein Prp5 is a member of the "DEAD box" family of putative RNA-dependent ATPases and helicases. The protein was purified from Escherichia coli and determined to be an RNA-dependent ATPase. The ATPase activity is 7-fold more specific for full-length U2 than for any of the other small nuclear RNAs or nonspecific RNAs tested. An RNaseH assay in extracts was used to demonstrate that Prp5 mediates an ATP-dependent conformational change in the intact U2 small nuclear ribonucleoprotein. We propose that this conformational change makes the branch point pairing sequence of U2 RNA accessible for pairing with the intron allowing formation of the pre-spliceosome.


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