| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, August 18, 1995; and in revised form, November 28, 1995)
Basal as well as induced transcription from the human urokinase-type plasminogen activator gene requires an enhancer containing two elements, a combined PEA3/AP-1 and a consensus AP-1 site. The integrity of each of these binding sites as well as their cooperation is required for activating transcription. The two elements are separated by a 74-base pair cooperation mediating (COM) region required for the cooperation between the transactivating sites. The COM region contains binding sites for four different unidentified urokinase-type plasminogen activator enhancer factors (UEF 1 to 4), all four required for correct COM activity. We have purified UEF3 from HeLa nuclear extracts by several chromatographic steps including DNA affinity purification. Purification and UV cross-linking data showed that UEF3 is a complex of three polypeptides (p40, p50, and p64). Amino acid sequence from one peptide of p64 was obtained, which showed no homology to other known proteins. Both crude and purified UEF3 specifically bound to the sequence TGACAG as shown by electrophoretic mobility shifts and methylation interference studies. DNA-binding specificity of purified UEF3 was identical to that of NIP, a non-characterized factor binding and regulating multiple AP-1-regulated promoters like stromelysin and interleukin-3. Thus UEF3 appears to be a general DNA-binding factor involved in modulating the transcriptional response of AP-1 containing promoters.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  V. M. Diaz, S. Mori, E. Longobardi, G. Menendez, C. Ferrai, R. A. Keough, A. Bachi, and F. Blasi p160 Myb-Binding Protein Interacts with Prep1 and Inhibits Its Transcriptional Activity Mol. Cell. Biol., November 15, 2007; 27(22): 7981 - 7990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Penkov, P. Di Rosa, L. Fernandez Diaz, V. Basso, E. Ferretti, F. Grassi, A. Mondino, and F. Blasi Involvement of Prep1 in the {alpha}{beta} T-Cell Receptor T-Lymphocytic Potential of Hematopoietic Precursors Mol. Cell. Biol., December 15, 2005; 25(24): 10768 - 10781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ferretti, F. Cambronero, S. Tumpel, E. Longobardi, L. M. Wiedemann, F. Blasi, and R. Krumlauf Hoxb1 Enhancer and Control of Rhombomere 4 Expression: Complex Interplay between PREP1-PBX1-HOXB1 Binding Sites Mol. Cell. Biol., October 1, 2005; 25(19): 8541 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brendolan, E. Ferretti, V. Salsi, K. Moses, S. Quaggin, F. Blasi, M. L. Cleary, and L. Selleri A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny Development, July 1, 2005; 132(13): 3113 - 3126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Benasciutti, G. Pages, O. Kenzior, W. Folk, F. Blasi, and M. P. Crippa MAPK and JNK transduction pathways can phosphorylate Sp1 to activate the uPA minimal promoter element and endogenous gene transcription Blood, July 1, 2004; 104(1): 256 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Longobardi and F. Blasi Overexpression of PREP-1 in F9 Teratocarcinoma Cells Leads to a Functionally Relevant Increase of PBX-2 by Preventing Its Degradation J. Biol. Chem., October 3, 2003; 278(40): 39235 - 39241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ibanez-Tallon, C. Ferrai, E. Longobardi, I. Facetti, F. Blasi, and M. P. Crippa Binding of Sp1 to the proximal promoter links constitutive expression of the human uPA gene and invasive potential of PC3 cells Blood, October 16, 2002; 100(9): 3325 - 3332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fognani, C. Kilstrup-Nielsen, J. Berthelsen, E. Ferretti, V. Zappavigna, and F. Blasi Characterization of PREP2, a paralog of PREP1, which defines a novel sub-family of the MEINOX TALE homeodomain transcription factors Nucleic Acids Res., May 1, 2002; 30(9): 2043 - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shimada, Y. Suzuki, S.-J. Kim, P.-C. Wang, M. Matsumura, and S. Kojima Transactivation via RAR/RXR-Sp1 Interaction: Characterization of Binding Between Sp1 and GC Box Motif Mol. Endocrinol., October 1, 2001; 15(10): 1677 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kojima, S. Hayashi, K. Shimokado, Y. Suzuki, J. Shimada, M. P. Crippa, and S. L. Friedman Transcriptional activation of urokinase by the Kruppel-like factor Zf9/COPEB activates latent TGF-beta 1 in vascular endothelial cells Blood, February 15, 2000; 95(4): 1309 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Ferretti, H Marshall, H Popperl, M Maconochie, R Krumlauf, and F Blasi Segmental expression of Hoxb2 in r4 requires two separate sites that integrate cooperative interactions between Prep1, Pbx and Hox proteins Development, January 1, 2000; 127(1): 155 - 166. [Abstract] [PDF]
|
|
|
|
|
|
G. Cirillo, L. Casalino, D. Vallone, A. Caracciolo, D. De Cesare, and P. Verde Role of Distinct Mitogen-Activated Protein Kinase Pathways and Cooperation between Ets-2, ATF-2, and Jun Family Members in Human Urokinase-Type Plasminogen Activator Gene Induction by Interleukin-1 and Tetradecanoyl Phorbol Acetate Mol. Cell. Biol., September 1, 1999; 19(9): 6240 - 6252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Suzuki, J. Shimada, K. Shudo, M. Matsumura, M. P. Crippa, and S. Kojima Physical Interaction Between Retinoic Acid Receptor and Sp1: Mechanism for Induction of Urokinase by Retinoic Acid Blood, June 15, 1999; 93(12): 4264 - 4276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Selvamurugan, W.-Y. Chou, A. T. Pearman, M. R. Pulumati, and N. C. Partridge Parathyroid Hormone Regulates the Rat Collagenase-3 Promoter in Osteoblastic Cells through the Cooperative Interaction of the Activator Protein-1 Site and the runt Domain Binding Sequence J. Biol. Chem., April 24, 1998; 273(17): 10647 - 10657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. De Cesare, M. Palazzolo, J. Berthelsen, and F. Blasi Characterization of UEF-4, a DNA-binding Protein Required for Transcriptional Synergism between Two AP-1 Sites in the Human Urokinase Enhancer J. Biol. Chem., September 19, 1997; 272(38): 23921 - 23929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Herzig, L. Fuzesi, and W. Knepel Heterodimeric Pbx-Prep1 Homeodomain Protein Binding to the Glucagon Gene Restricting Transcription in a Cell Type-dependent Manner J. Biol. Chem., September 1, 2000; 275(36): 27989 - 27999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, L. Yin, and F. B. Hillgartner The Homeodomain Proteins PBX and MEIS1 Are Accessory Factors That Enhance Thyroid Hormone Regulation of the Malic Enzyme Gene in Hepatocytes J. Biol. Chem., June 22, 2001; 276(26): 23838 - 23848. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
