The promoter and its upstream regulatory region of the mouse cellular retinoic acid-binding protein I (crabp-I) gene were examined in transgenic mouse embryos, a mouse embryonal carcinoma cell line P19, and a mouse embryonic fibroblast cell line 3T6. In transgenic mouse embryos, a -galactosidase reporter gene under the control of crabp-I promoter and its upstream regulatory region displayed a very specific pattern of expression characteristic of crabp-I gene expression during developmental stages. In tissue culture systems, the minimal promoter of this gene was identified, and regions containing positive and negative regulatory activities were dissected from the upstream 3-kilobase sequence using assays for transient reporter activity. It is concluded that the minimal promoter of the mouse crabp-I gene is located between 120 and 150 base pairs upstream from the transcription initiation site. Several cell type-specific positive and negative regulatory regions for this promoter have been identified. A region encoding a common negative regulatory activity in both P19 and 3T6 cells is also inhibitory to two heterologous promoters, and specific protein-DNA interactions between this DNA fragment and nuclear extracts of P19 and 3T6 are demonstrated by gel retardation experiments.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. Bi, X. Hu, H. H. Loh, and L.-N. Wei Regulation of Mouse {kappa} Opioid Receptor Gene Expression by Retinoids J. Neurosci., March 1, 2001; 21(5): 1590 - 1599. [Abstract] [Full Text] [PDF]

				A. L. Means, J. R. Thompson, and L. J. Gudas Transcriptional Regulation of the Cellular Retinoic Acid Binding Protein I Gene in F9 Teratocarcinoma Cells Cell Growth Differ., February 1, 2000; 11(2): 71 - 82. [Abstract] [Full Text]

				L.-N. Wei, X. Hu, J. Bi, and H. Loh Post-Transcriptional Regulation of Mouse kappa -Opioid Receptor Expression Mol. Pharmacol., February 1, 2000; 57(2): 401 - 408. [Abstract] [Full Text]

				C.-H. Lee, C. Chinpaisal, and L.-N. Wei A Novel Nuclear Receptor Heterodimerization Pathway Mediated by Orphan Receptors TR2 and TR4 J. Biol. Chem., September 25, 1998; 273(39): 25209 - 25215. [Abstract] [Full Text] [PDF]

				S. Lu, H. H. Loh, and L.-N. Wei Studies of Dual Promoters of Mouse kappa -Opioid Receptor Gene Mol. Pharmacol., September 1, 1997; 52(3): 415 - 420. [Abstract] [Full Text]

				L. Chang and L.-N. Wei Characterization of a Negative Response DNA Element in the Upstream Region of the Cellular Retinoic Acid-binding Protein-I Gene of the Mouse J. Biol. Chem., April 11, 1997; 272(15): 10144 - 10150. [Abstract] [Full Text] [PDF]