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Volume 272, Number 13, Issue of March 28, 1997 pp. 8113-8116
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
Histone H1b Phosphorylation Is Dependent upon Ongoing Transcription and Replication in Normal and ras-transformed Mouse Fibroblasts

(Received for publication, January 10, 1997, and in revised form, February 5, 1997)

Deborah N. Chadee Dagger § , C. David Allis par , Jim A. Wright Dagger § and James R. Davie §

From the Dagger  Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada, the § Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Manitoba R3E OW3, Canada, and the par  Department of Biology, University of Rochester, Rochester, New York, 14627

We have previously shown that mouse phosphorylated histone H1b (pH1b) was localized near nuclear sites that contained splicing factors. This observation suggested to us that pH1b was associated with transcribing chromatin. Here we investigated the relationship between phosphorylation of H1b and transcription. We demonstrate that treatment of normal or ras-transformed mouse fibroblasts with the transcription inhibitor actinomycin D for 70 min results in a dramatic decrease in the level of pH1b. Similar results were observed when transcription was inhibited by 5,6-dichloro-1-beta -D-ribofuranosylbenzimidazole (DRB). When DRB was removed, the level of pH1b was restored after 2 h. Treatment of the cells with aphidicolin, a potent inhibitor of replication, resulted in a marked decrease in the level of pH1b after 30 min. This is the first report showing a dependence of histone modification upon ongoing transcription and replication. Inhibition of transcription or replication may hinder accessibility of H1b to the H1 kinase, supporting the idea that pH1b is associated with transcribing chromatin. Phosphorylation of H1b may be required to maintain a more decondensed chromatin structure to facilitate transcription and replication processes.


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