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(Received for publication, October 25, 1996, and in revised form, January 13, 1997)
From the Department of Molecular and Medical Pharmacology and
Molecular Biology Institute, UCLA School of Medicine,
Los Angeles, California 90095
Integration of retroviral DNA can occur into many
sites on target DNA with a wide variation in preference. One factor
known to affect target site selection is integrase, the viral protein required for the integration reaction. In this study, assays that measure the distribution and frequency of retroviral DNA integration showed that purified integrases of human immunodeficiency virus type 1 (HIV-1) and feline immunodeficiency virus (FIV) had different patterns
of target site usage. The integrase domain involved in target site
selection was mapped by analyzing the integration pattern of chimeric
proteins formed between HIV-1 and FIV integrases and of deletion
variants of the two wild-type integrases. The results indicate that the
domain responsible for target site selection resides in the central
core region of integrase.
Volume 272, Number 13,
Issue of March 28, 1997
pp. 8361-8369
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
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