HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wendler, W. M.F. Articles by Winnacker, E.-L. Search for Related Content PubMed PubMed Citation Articles by Wendler, W. M.F. Articles by Winnacker, E.-L. Social Bookmarking                      What's this?

Volume 272, Number 13, Issue of March 28, 1997 pp. 8482-8489
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Identification of Pirin, a Novel Highly Conserved Nuclear Protein

(Received for publication, October 30, 1996, and in revised form, December 19, 1996)

Wolfgang M. F. Wendler , Elisabeth Kremmer ^§ , Reinhold Förster ^¶ and Ernst-Ludwig Winnacker

From the  Institut für Biochemie der Ludwig-Maximilians-Universität München, Genzentrum, Feodor-Lynen-Strasse 25, D-81377 München, Germany, the ^§ Gesellschaft für Strahlen forschung-National Research Center for Environment and Health, Institute of Immunology, Marchioninistrasse 25, D-81377 München, Germany, and the ^¶ Max-Delbrück-Center for Molecular Medicine, Robert Rössle Strasse 10, D-13122 Berlin-Buch, Germany

In this article we describe the molecular cloning of Pirin, a novel highly conserved 32-kDa protein consisting of 290 amino acids. Pirin was isolated by a yeast two-hybrid screen as an interactor of nuclear factor I/CCAAT box transcription factor (NFI/CTF1), which is known to stimulate adenovirus DNA replication and RNA polymerase II-driven transcription. Pirin mRNA is expressed weakly in all human tissues tested. About 15% of all Pirin cDNAs contain a short 34-base pair insertion in their 5-untranslated regions, indicative of alternative splicing processes. Multiple Pirin transcripts are expressed in skeletal muscle and heart. Western blots and immunoprecipitations employing monoclonal anti-Pirin antibodies reveal that Pirin is a nuclear protein. Moreover, confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Homology searches using the BLAST algorithm indicate the existence of Pirin homologues in mouse and rat. The N-terminal half of Pirin is significantly conserved between mammals, plants, fungi, and even prokaryotic organisms. Genomic Southern and Western blots demonstrate the presence of Pirin genes and their expression, respectively, in all mammalian cell lines tested. The expression pattern, the concentrated localization in subnuclear structures, and its interaction with NFI/CTF1 in the two-hybrid system classify Pirin as a putative NFI/CTF1 cofactor, which might help to gain new insights in NFI/CTF1 functions.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. M. Warpeha, S. Upadhyay, J. Yeh, J. Adamiak, S. I. Hawkins, Y. R. Lapik, M. B. Anderson, and L. S. Kaufman The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1590 - 1600. [Abstract] [Full Text] [PDF]

				P.-C. Soo, Y.-T. Horng, M.-J. Lai, J.-R. Wei, S.-C. Hsieh, Y.-L. Chang, Y.-H. Tsai, and H.-C. Lai Pirin Regulates Pyruvate Catabolism by Interacting with the Pyruvate Dehydrogenase E1 Subunit and Modulating Pyruvate Dehydrogenase Activity J. Bacteriol., January 1, 2007; 189(1): 109 - 118. [Abstract] [Full Text] [PDF]

				A. R. Mey, E. E. Wyckoff, V. Kanukurthy, C. R. Fisher, and S. M. Payne Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence Infect. Immun., December 1, 2005; 73(12): 8167 - 8178. [Abstract] [Full Text] [PDF]

				C. A. Parsons, H. J. Mroczkowski, F. E.A. McGuigan, O. M.E. Albagha, S. Manolagas, D. M. Reid, S. H. Ralston, and R. J. S. Reis Interspecies synteny mapping identifies a quantitative trait locus for bone mineral density on human chromosome Xp22 Hum. Mol. Genet., November 1, 2005; 14(21): 3141 - 3148. [Abstract] [Full Text] [PDF]

				M. Adams and Z. Jia Structural and Biochemical Analysis Reveal Pirins to Possess Quercetinase Activity J. Biol. Chem., August 5, 2005; 280(31): 28675 - 28682. [Abstract] [Full Text] [PDF]

				S. Bunda, N. Kaviani, and A. Hinek Fluctuations of Intracellular Iron Modulate Elastin Production J. Biol. Chem., January 21, 2005; 280(3): 2341 - 2351. [Abstract] [Full Text] [PDF]

				J. Zhang, A. Moseley, A. G. Jegga, A. Gupta, D. P. Witte, M. Sartor, M. Medvedovic, S. S. Williams, C. Ley-Ebert, L. M. Coolen, et al. Neural system-enriched gene expression: relationship to biological pathways and neurological diseases Physiol Genomics, July 8, 2004; 18(2): 167 - 183. [Abstract] [Full Text] [PDF]

				H. Pang, M. Bartlam, Q. Zeng, H. Miyatake, T. Hisano, K. Miki, L.-L. Wong, G. F. Gao, and Z. Rao Crystal Structure of Human Pirin: AN IRON-BINDING NUCLEAR PROTEIN AND TRANSCRIPTION COFACTOR J. Biol. Chem., January 9, 2004; 279(2): 1491 - 1498. [Abstract] [Full Text] [PDF]

				Y. R. Lapik and L. S. Kaufman The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein {alpha}-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development PLANT CELL, July 1, 2003; 15(7): 1578 - 1590. [Abstract] [Full Text] [PDF]

				V. S. Reddy, G. S. Ali, and A. S. N. Reddy Genes Encoding Calmodulin-binding Proteins in the Arabidopsis Genome J. Biol. Chem., March 15, 2002; 277(12): 9840 - 9852. [Abstract] [Full Text] [PDF]

				M. Matvienko, M. J. Torres, and J. I. Yoder Transcriptional Responses in the Hemiparasitic Plant Triphysaria versicolor to Host Plant Signals Plant Physiology, September 1, 2001; 127(1): 272 - 282. [Abstract] [Full Text] [PDF]

				J. M. Dunwell, S. Khuri, and P. J. Gane Microbial Relatives of the Seed Storage Proteins of Higher Plants: Conservation of Structure and Diversification of Function during Evolution of the Cupin Superfamily Microbiol. Mol. Biol. Rev., March 1, 2000; 64(1): 153 - 179. [Abstract] [Full Text] [PDF]

				L. Driller, R. J. Wellinger, M. Larrivee, E. Kremmer, S. Jaklin, and H. M. Feldmann A Short C-terminal Domain of Yku70p Is Essential for Telomere Maintenance J. Biol. Chem., August 4, 2000; 275(32): 24921 - 24927. [Abstract] [Full Text] [PDF]