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(Received for publication, June 20, 1996, and in revised form, December 3, 1996)
From the Only one bovine gene, corresponding to the human
cluster of genes FUT3-FUT5-FUT6, was found by Southern blot
analysis. The cognate bovine
Volume 272, Number 13,
Issue of March 28, 1997
pp. 8764-8773
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
(1,3)-Fucosyltransferase Gene Homologous to a Putative Ancestor
Gene of the Human FUT3-FUT5-FUT6 Cluster
,
,
,
Institut de Biotechnologie,
(1,3)-fucosyltransferase shares 67.3, 69.0, and 69.3% amino acid sequence identities with human FUC-T3,
FUC-T5, and FUC-T6 enzymes, respectively. As revealed by protein
sequence alignment, potential sites for asparagine-linked glycosylation and conserved cysteines, the bovine enzyme is an intermediate between
FUC-T3, FUC-T5, and FUC-T6 human enzymes. Transfected into COS-7 cells,
the bovine gene induced the synthesis of an (1,3)-fucosyltransferase
enzyme with type 2 substrate acceptor pattern specificity and induced
expression of fucosylated type 2 epitopes (Lex and
sialyl-Lex), but not of type 1 structures (Lea
or sialyl-Lea), suggesting that it has an acceptor
specificity similar to the human plasma FUC-T6. However, no enzyme
activity was detected in bovine plasma. Gene transcripts are detected
on tissues such as bovine liver, kidney, lung, and brain. The type 2 sialyl-Lex epitope was found in renal macula densa and
biliary ducts, and Lex and Ley epitopes were
detected on the brush border of epithelial cells of small and large
intestine, suggesting a tissue distribution closer to human FUC-T3, but
fucosylated type 1 structures (Lea, Leb, or
sialyl-Lea) were not detected at all in any bovine tissue.
Analysis of genetic distances on a combined phylogenetic tree of
fucosyltransferase genes suggests that the bovine gene is the
orthologous homologue of the ancestor of human genes constituting the
present FUT3-FUT5-FUT6 cluster.
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