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(Received for publication, October 2, 1996, and in revised form, December 20, 1996)
From the Department of Biochemistry, Institute of Chemistry,
University of São Paulo, Av. Prof. Lineu Prestes 748, São Paulo 05508-900, SP, Brazil
The induction of cellulases by cellulose, an
insoluble polymer, in the filamentous fungus Trichoderma reesei
is puzzling. We previously proposed a mechanism that is based on
the presence of low levels of cellulase in the uninduced fungus; this
basal cellulase activity would digest cellulose-releasing
oligosaccharides that could enter the cell and trigger expression of
cellulases. We now present experiments that lend further support to
this model. We show here that transcripts of two members of the
cellulase system, cbh1 and egl1, are present in
uninduced T. reesei cells. These transcripts are induced at
least 1100-fold in the presence of cellulose. We also show that a
construct containing the hygromycin B resistance-encoding gene driven
by the cbh1 promoter confers hygromycin B resistance to
T. reesei cells grown in the absence of cellulose.
Moreover, cellulose-induced production of the cbh1 transcript was suppressed when antisense RNA against three
members of the cellulase system was expressed in vivo.
Experiments are presented indicating that extracellular cellulase
activity is the rate-limiting event in induction of synthesis of the
cellulase transcripts by cellulose. The results reveal a critical
requirement for basal expression of the cellulase system for induction
of synthesis of its own transcripts by cellulose.
Volume 272, Number 15,
Issue of April 11, 1997
pp. 10169-10174
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
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