| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, January 15, 1997, and in revised form, February 11, 1997)
,
,
,
From the Chemokines are chemotactic proteins which play a
central role in immune and inflammatory responses. Chemokine receptors
are members of the seven transmembrane G-protein coupled family and have recently been shown to be involved in the entry of human immunodeficiency virus (HIV) into target cells. To study chemokine endocytosis in detail we have used novel site-specific chemistry to
make a fluorescently labeled CC-chemokine agonist (rhodamine-MIP-1
Cell Biology Unit, Glaxo Wellcome Medicines
Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY,
United Kingdom, the ¶ Departement de Biochimie Medicale, Centre
Medicale Universitaire, 1224 Champel, Geneva 1224, Switzerland,
Geneva Biomedical Research Institute, Glaxo Wellcome Research
and Development, 14 Chemin des Aulx, 1228 Plan les Ouates, Geneva 1228, Switzerland, and ** Affymax Research Institute, Palo Alto,
California 94304
) and antagonist (NBD-RANTES). We have also generated a CHO cell line
stably expressing a hemagglutinin-tagged version of the CC-chemokine receptor 1 (CCR1), and using these reagents we have examined the receptor-mediated endocytosis of CC-chemokines by confocal microscopy. Our studies reveal that the agonist was internalized and accumulated in
transferrin receptor-positive endosomes whereas the antagonist failed
to internalize. However, receptor-bound antagonist could be induced to
internalize by co-administration of agonist. Analysis of receptor
redistribution following chemokine addition confirmed that
sequestration was induced by agonists but not by antagonists.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Pastori, B. Weiser, C. Barassi, C. Uberti-Foppa, S. Ghezzi, R. Longhi, G. Calori, H. Burger, K. Kemal, G. Poli, et al. Long-lasting CCR5 internalization by antibodies in a subset of long-term nonprogressors: a possible protective effect against disease progression Blood, June 15, 2006; 107(12): 4825 - 4833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chun, C.-C. Yin, J.-Z. Song, M.-X. Liu, J.-H. Piao, Q. Lin, X.-B. Wang, and H.-L. Huang Soluble Expression of Recombinant Human Secondary Lymphoid Chemokine (SLC) in E. coli and Research on Its In Vitro and In Vivo Bioactivity J. Biochem., December 1, 2004; 136(6): 769 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ahlenstiel, R. P. Woitas, J. Rockstroh, and U. Spengler CC-chemokine receptor 5 (CCR5) in hepatitis C--at the crossroads of the antiviral immune response? J. Antimicrob. Chemother., June 1, 2004; 53(6): 895 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ge and J. S. Pachter Caveolin-1 Knockdown by Small Interfering RNA Suppresses Responses to the Chemokine Monocyte Chemoattractant Protein-1 by Human Astrocytes J. Biol. Chem., February 20, 2004; 279(8): 6688 - 6695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Veillard, B. Kwak, G. Pelli, F. Mulhaupt, R. W. James, A. E.I. Proudfoot, and F. Mach Antagonism of RANTES Receptors Reduces Atherosclerotic Plaque Formation in Mice Circ. Res., February 6, 2004; 94(2): 253 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rogez, M. Martin, N. Dereuddre-Bosquet, J. Martal, D. Dormont, and P. Clayette Anti-Human Immunodeficiency Virus Activity of Tau Interferon in Human Macrophages: Involvement of Cellular Factors and {beta}-Chemokines J. Virol., December 1, 2003; 77(23): 12914 - 12920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zoffmann, A. Chollet, and J.-L. Galzi Identification of the Extracellular Loop 2 as the Point of Interaction between the N Terminus of the Chemokine MIP-1alpha and Its CCR1 Receptor Mol. Pharmacol., September 1, 2002; 62(3): 729 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Chazaud, R. Ricoux, C. Christov, A. Plonquet, R. K. Gherardi, and G. Barlovatz-Meimon Promigratory Effect of Plasminogen Activator Inhibitor-1 on Invasive Breast Cancer Cell Populations Am. J. Pathol., January 1, 2002; 160(1): 237 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sauty, R. A. Colvin, L. Wagner, S. Rochat, F. Spertini, and A. D. Luster CXCR3 Internalization Following T Cell-Endothelial Cell Contact: Preferential Role of IFN-Inducible T Cell {alpha} Chemoattractant (CXCL11) J. Immunol., December 15, 2001; 167(12): 7084 - 7093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dulkys, C. Kluthe, T. Buschermohle, I. Barg, S. Kno{beta}, A. Kapp, A. E. I. Proudfoot, and J. Elsner IL-3 Induces Down-Regulation of CCR3 Protein and mRNA in Human Eosinophils J. Immunol., September 15, 2001; 167(6): 3443 - 3453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Gretz, C. C. Norbury, A. O. Anderson, A. E.I. Proudfoot, and S. Shaw Lymph-borne Chemokines and Other Low Molecular Weight Molecules Reach High Endothelial Venules via Specialized Conduits While a Functional Barrier Limits Access to the Lymphocyte Microenvironments in Lymph Node Cortex J. Exp. Med., November 13, 2000; 192(10): 1425 - 1440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Unutmaz, W. Xiang, M. J. Sunshine, J. Campbell, E. Butcher, and D. R. Littman The Primate Lentiviral Receptor Bonzo/STRL33 Is Coordinately Regulated with CCR5 and Its Expression Pattern Is Conserved Between Human and Mouse J. Immunol., September 15, 2000; 165(6): 3284 - 3292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. D. Zhang, A. V. Franco, T. Nguyen, C. P. Gray, and P. Hersey Differential Localization and Regulation of Death and Decoy Receptors for TNF-Related Apoptosis-Inducing Ligand (TRAIL) in Human Melanoma Cells J. Immunol., April 15, 2000; 164(8): 3961 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Richardson, B. C. Pridgen, B. Haribabu, and R. Snyderman Regulation of the Human Chemokine Receptor CCR1. CROSS-REGULATION BY CXCR1 AND CXCR2 J. Biol. Chem., March 24, 2000; 275(13): 9201 - 9208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Lee, M. E. Brummet, S. Shahabuddin, T. G. Woodworth, S. N. Georas, K. M. Leiferman, S. C. Gilman, C. Stellato, R. P. Gladue, R. P. Schleimer, et al. Cutaneous Injection of Human Subjects with Macrophage Inflammatory Protein-1{alpha} Induces Significant Recruitment of Neutrophils and Monocytes J. Immunol., March 15, 2000; 164(6): 3392 - 3401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Elsner, M. Mack, H. Bruhl, Y. Dulkys, D. Kimmig, G. Simmons, P. R. Clapham, D. Schlondorff, A. Kapp, T. N. C. Wells, et al. Differential Activation of CC Chemokine Receptors by AOP-RANTES J. Biol. Chem., March 10, 2000; 275(11): 7787 - 7794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jones, L. Tussey, N. Athanasou, and D. G. Jackson Heparan Sulfate Proteoglycan Isoforms of the CD44 Hyaluronan Receptor Induced in Human Inflammatory Macrophages Can Function as Paracrine Regulators of Fibroblast Growth Factor Action J. Biol. Chem., March 10, 2000; 275(11): 7964 - 7974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. I. Proudfoot, R. Buser, F. Borlat, S. Alouani, D. Soler, R. E. Offord, J.-M. Schroder, C. A. Power, and T. N. C. Wells Amino-terminally Modified RANTES Analogues Demonstrate Differential Effects on RANTES Receptors J. Biol. Chem., November 5, 1999; 274(45): 32478 - 32485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Clasper, S. Vekemans, M. Fiore, M. Plebanski, P. Wordsworth, G. David, and D. G. Jackson Inducible Expression of the Cell Surface Heparan Sulfate Proteoglycan Syndecan-2 (Fibroglycan) on Human Activated Macrophages Can Regulate Fibroblast Growth Factor Action J. Biol. Chem., August 20, 1999; 274(34): 24113 - 24123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Zimmermann, J. J. Conkright, and M. E. Rothenberg CC Chemokine Receptor-3 Undergoes Prolonged Ligand-induced Internalization J. Biol. Chem., April 30, 1999; 274(18): 12611 - 12618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Oppermann, M. Mack, A. E. I. Proudfoot, and H. Olbrich Differential Effects of CC Chemokines on CC Chemokine Receptor 5 (CCR5) Phosphorylation and Identification of Phosphorylation Sites on the CCR5 Carboxyl Terminus J. Biol. Chem., March 26, 1999; 274(13): 8875 - 8885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mariani, S. Wong, L. C. F. Mulder, D. A. Wilkinson, A. L. Reinhart, G. LaRosa, R. Nibbs, T. R. O'Brien, N. L. Michael, R. I. Connor, et al. CCR2-64I Polymorphism Is Not Associated with Altered CCR5 Expression or Coreceptor Function J. Virol., March 1, 1999; 73(3): 2450 - 2459. [Abstract] [Full Text]
|
|
|
|
|
|
C. J. Gordon, M. A. Muesing, A. E. I. Proudfoot, C. A. Power, J. P. Moore, and A. Trkola Enhancement of Human Immunodeficiency Virus Type 1 Infection by the CC-Chemokine RANTES Is Independent of the Mechanism of Virus-Cell Fusion J. Virol., January 1, 1999; 73(1): 684 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Madani, S. L. Kozak, M. P. Kavanaugh, and D. Kabat gp120 envelope glycoproteins of human immunodeficiency viruses competitively antagonize signaling by coreceptors CXCR4 and CCR5 PNAS, July 7, 1998; 95(14): 8005 - 8010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Ditzel, M. M. Rosenkilde, P. Garred, M. Wang, K. Koefoed, C. Pedersen, D. R. Burton, and T. W. Schwartz The CCR5 receptor acts as an alloantigen in CCR5Delta 32 homozygous individuals: Identification of chemokineand HIV-1-blocking human antibodies PNAS, April 28, 1998; 95(9): 5241 - 5245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mack, B. Luckow, P. J. Nelson, J. Cihak, G. Simmons, P. R. Clapham, N. Signoret, M. Marsh, M. Stangassinger, F. Borlat, et al. Aminooxypentane-RANTES Induces CCR5 Internalization but Inhibits Recycling: A Novel Inhibitory Mechanism of HIV Infectivity J. Exp. Med., April 20, 1998; 187(8): 1215 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Signoret, M. Rosenkilde, P. Klasse, T. Schwartz, M. Malim, J. Hoxie, and M Marsh Differential regulation of CXCR4 and CCR5 endocytosis J. Cell Sci., January 9, 1998; 111(18): 2819 - 2830. [Abstract] [PDF]
|
|
|
|
|
|
A. Trkola, W. A. Paxton, S. P. Monard, J. A. Hoxie, M. A. Siani, D. A. Thompson, L. Wu, C. R. Mackay, R. Horuk, and J. P. Moore Genetic Subtype-Independent Inhibition of Human Immunodeficiency Virus Type 1 Replication by CC and CXC Chemokines J. Virol., January 1, 1998; 72(1): 396 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Signoret, J. Oldridge, A. Pelchen-Matthews, P. J. Klasse, T. Tran, L. F. Brass, M. M. Rosenkilde, T. W. Schwartz, W. Holmes, W. Dallas, et al. Phorbol Esters and SDF-1 Induce Rapid Endocytosis and Down Modulation of the Chemokine Receptor CXCR4 J. Cell Biol., November 3, 1997; 139(3): 651 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Loetscher, A. Pellegrino, J.-H. Gong, I. Mattioli, M. Loetscher, G. Bardi, M. Baggiolini, and I. Clark-Lewis The Ligands of CXC Chemokine Receptor 3, I-TAC, Mig, and IP10, Are Natural Antagonists for CCR3 J. Biol. Chem., January 26, 2001; 276(5): 2986 - 2991. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
