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Volume 272, Number 16, Issue of April 18, 1997 pp. 10664-10668
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

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The Human Transcription Enhancer Factor-1, TEF-1, Can Substitute for Drosophila scalloped during Wingblade Development

(Received for publication, December 3, 1996, and in revised form, January 29, 1997)

Nirupama Deshpande , Abha Chopra , Annapoorni Rangarajan , L. S. Shashidhara , Veronica Rodrigues and Sudhir Krishna

From the National Centre for Biological Sciences, TIFR Center, Bangalore 560012, India and the  Molecular Biology Unit, Tata Institute of Fundamental Research, Bombay 400005, India

The human transcription enhancer factor-1 (TEF-1) belongs to a family of evolutionarily conserved proteins that have a DNA binding TEA domain. TEF-1 shares a 98% homology with Drosophila scalloped (sd) in the DNA binding domain and a 50% similarity in the activation domain. We have expressed human TEF-1 in Drosophila under the hsp-70 promoter and find that it can substitute for Sd function. The transformants rescue the wingblade defects as well as the lethality of loss-of-function alleles. Observation of reporter activity in the imaginal wing discs of the enhancer-trap alleles suggests that TEF-1 is capable of promoting sd gene regulation. The functional capability of the TEF-1 product was assessed by comparing the extent of rescue by heat shock (hs)-TEF-1 with that of hs-sd. The finding that TEF-1 can function in vivo during wingblade development offers a potent genetic system for the analysis of its function and in the identification of the molecular partners of TEF-1.

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