|
|
|
|||||||
|
|||||||||
(Received for publication, October 10, 1996, and in revised form, January 2, 1997)
From the The vacuolar type proton pump of clathrin-coated
vesicles has a multisubunit ATP hydrolytic center that is peripheral to
the membrane. Polypeptides present in this domain include the well characterized subunits A, B, C, D, E, and F; SFD, a dimer composed of
50- and 57-kDa polypeptides; and polypeptides termed G and H. Of these,
subunits A, B, C, and E have been shown to be necessary but not
sufficient for significant ATPase activity; in addition, either
polypeptide G or H is also required for ATP hydrolysis (Xie, X.-S.
(1996) J. Biol. Chem. 271, 30980-30985).
In this study, the polypeptides G and H were purified and directly
sequenced. Subsequent molecular analysis has revealed that these
proteins are isoforms, which we designate G1 and G2. The cDNAs
encoding the rat and bovine brain and chicken osteoclast forms of G1
have been cloned. The open reading frames of the rat and bovine clones
encode hydrophilic proteins of 118 amino acids that differ at only five
residues; bovine G1 has 36% identity with VMA10, a component of the
proton channel of yeast. Northern blot analysis revealed a 1.0-kilobase
pair transcript encoding G1 in bovine brain, kidney, heart, and spleen.
The cDNA encoding bovine polypeptide H was cloned and sequenced,
revealing this protein to be 64% identical to G1, constituting isoform
G2. In Northern blot analysis, a single 1.7-kilobase pair transcript hybridized with a probe to G2 in brain, but not in heart, kidney, or
spleen. An antibody against a bovine G1-specific domain reacts with V
pump from bovine brain, kidney, and chromaffin granule, whereas an
anti-G2 antibody reacts only with proton pump from brain. The bovine
forms of G1 and G2 were subsequently expressed in Escherichia
coli and Sf9 cells, respectively, and purified to homogeneity.
Reconstitution of ATP hydrolysis was achieved by combination of
recombinant subunits A, B, C, and E with either recombinant G1 or G2,
demonstrating the role of these isoforms in pump function.
Volume 272, Number 16,
Issue of April 18, 1997
pp. 10721-10728
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
MOLECULAR CHARACTERIZATION AND FUNCTIONAL EXPRESSION
,§¶
,
,
,
,
,
and
Division of Molecular Transport, Department
of Internal Medicine, University of Texas Southwestern Medical Center,
Dallas, Texas 75235-9121, § Preclinical Research and
Development, Astra Hässle AB, S-431 83, Mölndal, Sweden,
and the ¶ Department of Biochemistry and Biophysics,
Göteborg University, Medicinaregatan, 9C, S-41390,
Göteborg, Sweden
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Su, K. G. Blake-Palmer, S. Sorrell, B. Javid, K. Bowers, A. Zhou, S. H. Chang, S. Qamar, and F. E. Karet Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1 Am J Physiol Renal Physiol, October 1, 2008; 295(4): F950 - F958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Breton and D. Brown New insights into the regulation of V-ATPase-dependent proton secretion Am J Physiol Renal Physiol, January 1, 2007; 292(1): F1 - F10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pietrement, G-H. Sun-Wada, N. D. Silva, M. McKee, V. Marshansky, D. Brown, M. Futai, and S. Breton Distinct Expression Patterns of Different Subunit Isoforms of the V-ATPase in the Rat Epididymis Biol Reprod, January 1, 2006; 74(1): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. L. Chaban, U. Coskun, W. Keegstra, G. T. Oostergetel, E. J. Boekema, and G. Gruber Structural Characterization of an ATPase Active F1-/V1 -ATPase ({alpha}3{beta}3EG) Hybrid Complex J. Biol. Chem., November 12, 2004; 279(46): 47866 - 47870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fethiere, D. Venzke, M. Diepholz, A. Seybert, A. Geerlof, M. Gentzel, M. Wilm, and B. Bottcher Building the Stator of the Yeast Vacuolar-ATPase: SPECIFIC INTERACTION BETWEEN SUBUNITS E AND G J. Biol. Chem., September 24, 2004; 279(39): 40670 - 40676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Murata, G.-H. Sun-Wada, T. Yoshimizu, A. Yamamoto, Y. Wada, and M. Futai Differential Localization of the Vacuolar H+ Pump with G Subunit Isoforms (G1 and G2) in Mouse Neurons J. Biol. Chem., September 20, 2002; 277(39): 36296 - 36303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Smith, K. E. Finberg, C. A. Wagner, R. P. Lifton, M. A. J. Devonald, Y. Su, and F. E. Karet Molecular Cloning and Characterization of Atp6n1b. A NOVEL FOURTH MURINE VACUOLAR H+-ATPase a-SUBUNIT GENE J. Biol. Chem., November 2, 2001; 276(45): 42382 - 42388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yokoyama, S. Ohkuma, H. Taguchi, T. Yasunaga, T. Wakabayashi, and M. Yoshida V-Type H+-ATPase/Synthase from a Thermophilic Eubacterium, Thermus Thermophilus. SUBUNIT STRUCTURE AND OPERON J. Biol. Chem., April 28, 2000; 275(18): 13955 - 13961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Toyomura, T. Oka, C. Yamaguchi, Y. Wada, and M. Futai Three Subunit a Isoforms of Mouse Vacuolar H+-ATPase. PREFERENTIAL EXPRESSION OF THE a3 ISOFORM DURING OSTEOCLAST DIFFERENTIATION J. Biol. Chem., March 17, 2000; 275(12): 8760 - 8765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawamura, K. Arakawa, M. Maeshima, and S. Yoshida Tissue Specificity of E Subunit Isoforms of Plant Vacuolar H+-ATPase and Existence of Isotype Enzymes J. Biol. Chem., February 25, 2000; 275(9): 6515 - 6522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bowman and B. Bowman Cellular role of the V-ATPase in Neurospora crassa: analysis of mutants resistant to concanamycin or lacking the catalytic subunit A J. Exp. Biol., January 1, 2000; 203(1): 97 - 106. [Abstract]
|
|
|
|
|
|
Z. Zhou, S.-B. Peng, B. P. Crider, P. Andersen, X.-S. Xie, and D. K. Stone Recombinant SFD Isoforms Activate Vacuolar Proton Pumps J. Biol. Chem., May 28, 1999; 274(22): 15913 - 15919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Forgac Structure and Properties of the Vacuolar (H+)-ATPases J. Biol. Chem., May 7, 1999; 274(19): 12951 - 12954. [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Neville and R. D. Campbell A New Member of the Ig Superfamily and a V-ATPase G Subunit Are Among the Predicted Products of Novel Genes Close to the TNF Locus in the Human MHC J. Immunol., April 15, 1999; 162(8): 4745 - 4754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nelson and W. R. Harvey Vacuolar and Plasma Membrane Proton-Adenosinetriphosphatases Physiol Rev, April 1, 1999; 79(2): 361 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-B. Peng, X. Li, B. P. Crider, Z. Zhou, P. Andersen, S. J. Tsai, X.-S. Xie, and D. K. Stone Identification and Reconstitution of an Isoform of the 116-kDa Subunit of the Vacuolar Proton Translocating ATPase J. Biol. Chem., January 22, 1999; 274(4): 2549 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ludwig, S. Kerscher, U. Brandt, K. Pfeiffer, F. Getlawi, D. K. Apps, and H. Schagger Identification and Characterization of a Novel 9.2-kDa Membrane Sector-associated Protein of Vacuolar Proton-ATPase from Chromaffin Granules J. Biol. Chem., May 1, 1998; 273(18): 10939 - 10947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tomashek, L. A. Graham, M. U. Hutchins, T. H. Stevens, and D. J. Klionsky V1-situated Stalk Subunits of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., October 17, 1997; 272(42): 26787 - 26793. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |