| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, November 13, 1996, and in revised form, January 27, 1997)
From the Department of Pharmacology and Toxicology, University of
Kansas, Lawrence, Kansas 66045
Caveolae are plasma membrane microdomains that
are enriched in caveolin, the structural protein of caveolae,
sphingomyelin, and other signaling molecules. We previously suggested
that neurotrophin-induced p75NTR-dependent
sphingomyelin hydrolysis may be localized to the plasma membrane.
Therefore, we examined if caveolae were a major site of
p75NTR-dependent sphingomyelin hydrolysis in
p75NTR-NIH 3T3 fibroblasts. Caveolin-enriched membranes (CEMs)
were prepared by either detergent or detergent-free extraction and separated from noncaveolar membranes by centrifugation through sucrose
gradients. Immunoblot analysis of the individual gradient fractions
indicated that caveolin and p75NTR were enriched in CEMs. The
localization of p75NTR to CEMs was not an artifact of receptor
overexpression in the fibroblasts because a similar distribution of
p75NTR was evident from PC12 cells, which endogenously express
p75NTR. In the p75NTR fibroblasts, nerve growth factor
induced a time-dependent hydrolysis of sphingomyelin only
in CEMs with no hydrolysis detected in noncaveolar membranes.
Intriguingly, endogenous p75NTR was found to
co-immunoprecipitate with caveolin, suggesting that p75NTR may
associate with caveolin in vivo. This interaction was
confirmed in vitro by the co-immunoprecipitation of a
glutathione S-transferase fusion protein expressing the
cytoplasmic domain of p75NTR with caveolin. Collectively, these
results demonstrate that neurotrophin-induced p75NTR-dependent sphingomyelin hydrolysis localizes
to CEMs and suggest that the interaction of p75NTR with
caveolin may affect signaling through p75NTR.
Volume 272, Number 16,
Issue of April 18, 1997
pp. 10922-10927
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Martin, D. C. Phillips, K. Szekely-Szucs, L. Elghazi, F. Desmots, and J. A. Houghton Cyclooxygenase-2 Inhibition Sensitizes Human Colon Carcinoma Cells to TRAIL-Induced Apoptosis through Clustering of DR5 and Concentrating Death-Inducing Signaling Complex Components into Ceramide-Enriched Caveolae Cancer Res., December 15, 2005; 65(24): 11447 - 11458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yu, M. Alterman, and R. T. Dobrowsky Ceramide displaces cholesterol from lipid rafts and decreases the association of the cholesterol binding protein caveolin-1 J. Lipid Res., August 1, 2005; 46(8): 1678 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Wood, J. S. Nye, N. J. C. Lamb, A. Fernandez, and M. Kitzmann Intracellular Retention of Caveolin 1 in Presenilin-deficient Cells J. Biol. Chem., February 25, 2005; 280(8): 6663 - 6668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Ding and J. L. Staudinger Induction of Drug Metabolism by Forskolin: The Role of the Pregnane X Receptor and the Protein Kinase A Signal Transduction Pathway J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 849 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishio, S. Fukumoto, K. Furukawa, A. Ichimura, H. Miyazaki, S. Kusunoki, T. Urano, and K. Furukawa Overexpressed GM1 Suppresses Nerve Growth Factor (NGF) Signals by Modulating the Intracellular Localization of NGF Receptors and Membrane Fluidity in PC12 Cells J. Biol. Chem., August 6, 2004; 279(32): 33368 - 33378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cabrera-Poch, L. Sanchez-Ruiloba, M. Rodriguez-Martinez, and T. Iglesias Lipid Raft Disruption Triggers Protein Kinase C and Src-dependent Protein Kinase D Activation and Kidins220 Phosphorylation in Neuronal Cells J. Biol. Chem., July 2, 2004; 279(27): 28592 - 28602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bate, M. Salmona, L. Diomede, and A. Williams Squalestatin Cures Prion-infected Neurons and Protects Against Prion Neurotoxicity J. Biol. Chem., April 9, 2004; 279(15): 14983 - 14990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Aouad, L. Y. Cohen, E. Sharif-Askari, E. K. Haddad, A. Alam, and R.-P. Sekaly Caspase-3 Is a Component of Fas Death-Inducing Signaling Complex in Lipid Rafts and Its Activity Is Required for Complete Caspase-8 Activation during Fas-Mediated Cell Death J. Immunol., February 15, 2004; 172(4): 2316 - 2323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. C. Bender, J. C. Whitbeck, M. Ponce de Leon, H. Lou, R. J. Eisenberg, and G. H. Cohen Specific Association of Glycoprotein B with Lipid Rafts during Herpes Simplex Virus Entry J. Virol., September 1, 2003; 77(17): 9542 - 9552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ha, H. B. Kwak, S. K. Lee, D. S. Na, C. E. Rudd, Z. H. Lee, and H.-H. Kim Membrane Rafts Play a Crucial Role in Receptor Activator of Nuclear Factor kappa B Signaling and Osteoclast Function J. Biol. Chem., May 9, 2003; 278(20): 18573 - 18580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Pike Lipid rafts: bringing order to chaos J. Lipid Res., April 1, 2003; 44(4): 655 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Czarny, J. Liu, P. Oh, and J. E. Schnitzer Transient Mechanoactivation of Neutral Sphingomyelinase in Caveolae to Generate Ceramide J. Biol. Chem., February 7, 2003; 278(7): 4424 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Liu, M. Rudick, and R. G. W. Anderson Multiple Functions of Caveolin-1 J. Biol. Chem., October 25, 2002; 277(44): 41295 - 41298. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Razani, S. E. Woodman, and M. P. Lisanti Caveolae: From Cell Biology to Animal Physiology Pharmacol. Rev., September 1, 2002; 54(3): 431 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Cottin, J. E. S. Doan, and D. W. H. Riches Restricted Localization of the TNF Receptor CD120a to Lipid Rafts: A Novel Role for the Death Domain J. Immunol., April 15, 2002; 168(8): 4095 - 4102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lipardi, G. Ruggiano, L. Perrone, S. Paladino, L. Monlauzeur, L. Nitsch, A. Le Bivic, and C. Zurzolo Differential Recognition of a Tyrosine-Dependent Signal in the Basolateral and Endocytic Pathways of Thyroid Epithelial Cells Endocrinology, April 1, 2002; 143(4): 1291 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Brann, M. Tcherpakov, I. M. Williams, A. H. Futerman, and M. Fainzilber Nerve Growth Factor-induced p75-mediated Death of Cultured Hippocampal Neurons Is Age-dependent and Transduced through Ceramide Generated by Neutral Sphingomyelinase J. Biol. Chem., March 15, 2002; 277(12): 9812 - 9818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Grassme, V. Jendrossek, J. Bock, A. Riehle, and E. Gulbins Ceramide-Rich Membrane Rafts Mediate CD40 Clustering J. Immunol., January 1, 2002; 168(1): 298 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, P. Lee, F. Galbiati, R. N. Kitsis, and M. P. Lisanti Caveolin-1 expression sensitizes fibroblastic and epithelial cells to apoptotic stimulation Am J Physiol Cell Physiol, April 1, 2001; 280(4): C823 - C835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bibel and Y.-A. Barde Neurotrophins: key regulators of cell fate and cell shape in the vertebrate nervous system Genes & Dev., December 1, 2000; 14(23): 2919 - 2937. [Full Text]
|
|
|
|
|
|
E. Klapisz, J. Masliah, G. Béréziat, C. Wolf, and K. S. Koumanov Sphingolipids and cholesterol modulate membrane susceptibility to cytosolic phospholipase A2 J. Lipid Res., October 1, 2000; 41(10): 1680 - 1688. [Abstract] [Full Text]
|
|
|
|
|
|
A. Prinetti, V. Chigorno, G. Tettamanti, and S. Sonnino Sphingolipid-enriched Membrane Domains from Rat Cerebellar Granule Cells Differentiated in Culture. A COMPOSITIONAL STUDY J. Biol. Chem., April 14, 2000; 275(16): 11658 - 11665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-s. Huang, J. Zhou, A. K. Feng, C. C. Lynch, J. Klumperman, S. J. DeArmond, and W. C. Mobley Nerve Growth Factor Signaling in Caveolae-like Domains at the Plasma Membrane J. Biol. Chem., December 17, 1999; 274(51): 36707 - 36714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Smart, G. A. Graf, M. A. McNiven, W. C. Sessa, J. A. Engelman, P. E. Scherer, T. Okamoto, and M. P. Lisanti Caveolins, Liquid-Ordered Domains, and Signal Transduction Mol. Cell. Biol., November 1, 1999; 19(11): 7289 - 7304. [Full Text] [PDF]
|
|
|
|
 |
|
 Rosie Xing and R. Kolesnick Stress signaling of apoptosis via ceramide and c-jun kinase Innate Immunity, August 1, 1999; 5(4): 216 - 221. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Vesper, E.-M. Schmelz, M. N. Nikolova-Karakashian, D. L. Dillehay, D. V. Lynch, and A. H. Merrill Jr. Sphingolipids in Food and the Emerging Importance of Sphingolipids to Nutrition J. Nutr., July 1, 1999; 129(7): 1239 - 1250. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-G. Ko, J.-S. Lee, Y.-S. Kang, J.-H. Ahn, and J.-S. Seo TNF-{alpha}-Mediated Apoptosis Is Initiated in Caveolae-Like Domains J. Immunol., June 15, 1999; 162(12): 7217 - 7223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. MacKichan and A. L. DeFranco Role of Ceramide in Lipopolysaccharide (LPS)-induced Signaling. LPS INCREASES CERAMIDE RATHER THAN ACTING AS A STRUCTURAL HOMOLOG J. Biol. Chem., January 15, 1999; 274(3): 1767 - 1775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Bilderback, V.-R. Gazula, M. P. Lisanti, and R. T. Dobrowsky Caveolin Interacts with Trk A and p75NTR and Regulates Neurotrophin Signaling Pathways J. Biol. Chem., January 1, 1999; 274(1): 257 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W. Shaul and R. G. W. Anderson Role of plasmalemmal caveolae in signal transduction Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L843 - L851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Galbiati, D. Volonte, O. Gil, G. Zanazzi, J. L. Salzer, M. Sargiacomo, P. E. Scherer, J. A. Engelman, A. Schlegel, M. Parenti, et al. Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: Caveolin-2 is up-regulated in response to cell injury PNAS, August 18, 1998; 95(17): 10257 - 10262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Waugh, D. Lawson, S. K. Tan, and J. J. Hsuan Phosphatidylinositol 4-Phosphate Synthesis in Immunoisolated Caveolae-like Vesicles and Low Buoyant Density Non-caveolar Membranes J. Biol. Chem., July 3, 1998; 273(27): 17115 - 17121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Lin, M. O. Hengartner, and R. Kolesnick Caenorhabditis elegans Contains Two Distinct Acid Sphingomyelinases J. Biol. Chem., June 5, 1998; 273(23): 14374 - 14379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Okamoto, A. Schlegel, P. E. Scherer, and M. P. Lisanti Caveolins, a Family of Scaffolding Proteins for Organizing "Preassembled Signaling Complexes" at the Plasma Membrane J. Biol. Chem., March 6, 1998; 273(10): 5419 - 5422. [Full Text] [PDF]
|
|
|
|
|
|
T. Ariga, W. D. Jarvis, and R. K. Yu Role of sphingolipid-mediated cell death in neurodegenerative diseases J. Lipid Res., January 1, 1998; 39(1): 1 - 16. [Abstract] [Full Text]
|
|
|
|
|
|
S. Peiro, J. X. Comella, C. Enrich, D. Martin-Zanca, and N. Rocamora PC12 Cells Have Caveolae That Contain TrkA. CAVEOLAE-DISRUPTING DRUGS INHIBIT NERVE GROWTH FACTOR-INDUCED, BUT NOT EPIDERMAL GROWTH FACTOR-INDUCED, MAPK PHOSPHORYLATION J. Biol. Chem., November 22, 2000; 275(48): 37846 - 37852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Prabhakar, V. Cheng, and T. Michel A Chimeric Transmembrane Domain Directs Endothelial Nitric-oxide Synthase Palmitoylation and Targeting to Plasmalemmal Caveolae J. Biol. Chem., June 16, 2000; 275(25): 19416 - 19421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, J. H. Bauer, Y. Li, Z. Shao, F. S. Zetoune, E. Cattaneo, and C. Vincenz Characterization of a p75NTR Apoptotic Signaling Pathway Using a Novel Cellular Model J. Biol. Chem., August 31, 2001; 276(36): 33812 - 33820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Roux, A. L. Bhakar, T. E. Kennedy, and P. A. Barker The p75 Neurotrophin Receptor Activates Akt (Protein Kinase B) through a Phosphatidylinositol 3-Kinase-dependent Pathway J. Biol. Chem., June 15, 2001; 276(25): 23097 - 23104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Grassme, A. Jekle, A. Riehle, H. Schwarz, J. Berger, K. Sandhoff, R. Kolesnick, and E. Gulbins CD95 Signaling via Ceramide-rich Membrane Rafts J. Biol. Chem., June 1, 2001; 276(23): 20589 - 20596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cremesti, F. Paris, H. Grassme, N. Holler, J. Tschopp, Z. Fuks, E. Gulbins, and R. Kolesnick Ceramide Enables Fas to Cap and Kill J. Biol. Chem., June 22, 2001; 276(26): 23954 - 23961. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
