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(Received for publication, October 21, 1996, and in revised form, February 11, 1997)
From the The Saccharomyces cerevisiae Tup1
protein is a member of a family of WD repeat containing proteins that
are involved in repression of transcription. Tup1, along with the Ssn6
protein, represses a wide variety of genes in yeast including cell
type-specific and glucose-repressed genes. Tup1 and Ssn6 are recruited
to these specific gene sets by interaction with sequence-specific DNA
binding proteins. In this work, a protein complex containing Ssn6 and Tup1 was purified to determine its composition. The size of the complex
is estimated to be 440 kDa. Tup1 and Ssn6, which are both phosphoproteins, are the only proteins present in stoichiometric amounts in the complex. We also demonstrate that this purified complex
represses transcription in an in vitro assay.
Volume 272, Number 17,
Issue of April 25, 1997
pp. 11193-11197
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,
and§
Department of Biochemistry and Biophysics
and the § Department of Microbiology and Immunology,
University of California, San Francisco, California 94143-0414
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