JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, S.
Right arrow Articles by Grosse, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, S.
Right arrow Articles by Grosse, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 17, Issue of April 25, 1997 pp. 11487-11494
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Domain Structure of Human Nuclear DNA Helicase II (RNA Helicase A)

(Received for publication, October 22, 1996, and in revised form, February 19, 1997)

Suisheng Zhang and Frank Grosse

From Abteilung Biochemie, Institut für Molekulare Biotechnologie, Postfach 100813, D-07708 Jena, Germany

Full-length human nuclear DNA helicase II (NDH II) was cloned and overexpressed in a baculovirus-derived expression system. Recombinant NDH II unwound both DNA and RNA. Limited tryptic digestion produced active helicases with molecular masses of 130 and 100 kDa. The 130-kDa helicase missed a glycine-rich domain (RGG-box) at the carboxyl terminus, while the 100-kDa form missed both its double-stranded RNA binding domains (dsRBDs) at the amino terminus and its RGG-box. Hence, the dsRBDs and the RGG-box were dispensable for unwinding. On the other hand, the isolated DEXH core alone could neither hydrolyze ATP nor unwind nucleic acids. These enzymatic activities were not regained by fusing a complete COOH or NH2 terminus to the helicase core. Hence, an active helicase required part of the NH2 terminus, the DEXH core, and a C-terminal extension of the core. Both dsRBDs and the RGG-box were bacterially expressed as glutathione S-transferase fusion proteins. The two dsRBDs had a strong affinity to double-stranded RNA and cooperated upon RNA binding, while the RGG-box bound preferentially to single-stranded DNA. A model is suggested in which the flanking domains influence and regulate the unwinding properties of NDH II.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Izzo, C. Regnard, V. Morales, E. Kremmer, and P. B. Becker
Structure-function analysis of the RNA helicase maleless
Nucleic Acids Res., February 11, 2008; 36(3): 950 - 962.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Valineva, J. Yang, and O. Silvennoinen
Characterization of RNA helicase A as component of STAT6-dependent enhanceosome
Nucleic Acids Res., September 1, 2006; 34(14): 3938 - 3946.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. Harris, Z. Zhang, B. Chaubey, and V. N. Pandey
Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome
Mol. Cell. Proteomics, June 1, 2006; 5(6): 1006 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. B. Roy, J. Hu, X. Guo, R. S. Russell, F. Guo, L. Kleiman, and C. Liang
Association of RNA Helicase A with Human Immunodeficiency Virus Type 1 Particles
J. Biol. Chem., May 5, 2006; 281(18): 12625 - 12635.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Friedemann, F. Grosse, and S. Zhang
Nuclear DNA Helicase II (RNA Helicase A) Interacts with Werner Syndrome Helicase and Stimulates Its Exonuclease Activity
J. Biol. Chem., September 2, 2005; 280(35): 31303 - 31313.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. E. Mischo, P. Hemmerich, F. Grosse, and S. Zhang
Actinomycin D Induces Histone {gamma}-H2AX Foci and Complex Formation of {gamma}-H2AX with Ku70 and Nuclear DNA Helicase II
J. Biol. Chem., March 11, 2005; 280(10): 9586 - 9594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. Iqbal, W. G. Sanger, D. E. Horsman, A. Rosenwald, D. L. Pickering, B. Dave, S. Dave, L. Xiao, K. Cao, Q. Zhu, et al.
BCL2 Translocation Defines a Unique Tumor Subset within the Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma
Am. J. Pathol., July 1, 2004; 165(1): 159 - 166.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhong and A. R. Safa
RNA Helicase A in the MEF1 Transcription Factor Complex Up-regulates the MDR1 Gene in Multidrug-resistant Cancer Cells
J. Biol. Chem., April 23, 2004; 279(17): 17134 - 17141.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Zhang, B. Schlott, M. Gorlach, and F. Grosse
DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner
Nucleic Acids Res., January 2, 2004; 32(1): 1 - 10.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Henning, R. B. So, R. Jin, L. F. Lau, and B. C. Valdez
Silencing of RNA Helicase II/Gu{alpha} Inhibits Mammalian Ribosomal RNA Production
J. Biol. Chem., December 26, 2003; 278(52): 52307 - 52314.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M.-L. Hung, P. Chao, and K.-Y. Chang
dsRBM1 and a proline-rich domain of RNA helicase A can form a composite binder to recognize a specific dsDNA
Nucleic Acids Res., October 1, 2003; 31(19): 5741 - 5753.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
L. R. SAUNDERS and G. N. BARBER
The dsRNA binding protein family: critical roles, diverse cellular functions
FASEB J, June 1, 2003; 17(9): 961 - 983.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
T. W. REICHMAN and M. B. MATHEWS
RNA binding and intramolecular interactions modulate the regulation of gene expression by nuclear factor 110
RNA, May 1, 2003; 9(5): 543 - 554.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Zhang, K. Buder, C. Burkhardt, B. Schlott, M. Gorlach, and F. Grosse
Nuclear DNA Helicase II/RNA Helicase A Binds to Filamentous Actin
J. Biol. Chem., January 4, 2002; 277(1): 843 - 853.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
S. Aratani, R. Fujii, T. Oishi, H. Fujita, T. Amano, T. Ohshima, M. Hagiwara, A. Fukamizu, and T. Nakajima
Dual Roles of RNA Helicase A in CREB-Dependent Transcription
Mol. Cell. Biol., July 15, 2001; 21(14): 4460 - 4469.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Vindigni, A. Ochem, G. Triolo, and A. Falaschi
Identification of human DNA helicase V with the far upstream element-binding protein
Nucleic Acids Res., March 1, 2001; 29(5): 1061 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. Ohlstein, C. A. Lavoie, O. Vef, E. Gateff, and D. M. McKearin
The Drosophila Cystoblast Differentiation Factor, benign gonial cell neoplasm, Is Related to DExH-box Proteins and Interacts Genetically With bag-of-marbles
Genetics, August 1, 2000; 155(4): 1809 - 1819.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
Y. Takeda, P. Caudell, G. Grady, G. Wang, A. Suwa, G. C. Sharp, W. S. Dynan, and J. A. Hardin
Human RNA Helicase A Is a Lupus Autoantigen That Is Cleaved During Apoptosis
J. Immunol., December 1, 1999; 163(11): 6269 - 6274.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Tang, D. McDonald, T. Middlesworth, T. J. Hope, and F. Wong-Staal
The Carboxyl Terminus of RNA Helicase A Contains a Bidirectional Nuclear Transport Domain
Mol. Cell. Biol., May 1, 1999; 19(5): 3540 - 3550.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S Zhang, C Herrmann, and F Grosse
Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A)
J. Cell Sci., January 8, 1999; 112(16): 2693 - 2703.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S Zhang, C Herrmann, and F Grosse
Pre-mRNA and mRNA binding of human nuclear DNA helicase II (RNA helicase A)
J. Cell Sci., January 4, 1999; 112(7): 1055 - 1064.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. Myohanen and S. B. Baylin
Sequence-specific DNA Binding Activity of RNA Helicase A to the p16INK4a Promoter
J. Biol. Chem., January 5, 2001; 276(2): 1634 - 1642.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Fujii, M. Okamoto, S. Aratani, T. Oishi, T. Ohshima, K. Taira, M. Baba, A. Fukamizu, and T. Nakajima
A Role of RNA Helicase A in cis-Acting Transactivation Response Element-mediated Transcriptional Regulation of Human Immunodeficiency Virus Type 1
J. Biol. Chem., February 16, 2001; 276(8): 5445 - 5451.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.