JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dix, D.
Right arrow Articles by Eide, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dix, D.
Right arrow Articles by Eide, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 18, Issue of May 2, 1997 pp. 11770-11777
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Characterization of the FET4 Protein of Yeast
EVIDENCE FOR A DIRECT ROLE IN THE TRANSPORT OF IRON

(Received for publication, May 13, 1996, and in revised form, February 25, 1997)

David Dix , Jamie Bridgham , Margaret Broderius and David Eide

From the Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth, Minnesota 55812

The low affinity Fe2+ uptake system of Saccharomyces cerevisiae requires the FET4 gene. In this report, we present evidence that FET4 encodes the Fe2+ transporter protein of this system. Antibodies prepared against FET4 detected two distinct proteins with molecular masses of 63 and 68 kDa. In vitro synthesis of FET4 suggested that the 68-kDa form is the primary translation product, and the 63-kDa form may be generated by proteolytic cleavage of the full-length protein. Consistent with its role as an Fe2+ transporter, FET4 is an integral membrane protein present in the plasma membrane. The level of FET4 closely correlated with uptake activity over a broad range of expression levels and is itself regulated by iron. Furthermore, mutations in FET4 can alter the kinetic properties of the low affinity uptake system, suggesting a direct interaction between FET4 and its Fe2+ substrate. Mutations affecting potential Fe2+ ligands located in the predicted transmembrane domains of FET4 significantly altered the apparent Km and/or Vmax of the low affinity system. These mutations may identify residues involved in Fe2+ binding during transport.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. S. Stoj, A. J. Augustine, E. I. Solomon, and D. J. Kosman
Structure-Function Analysis of the Cuprous Oxidase Activity in Fet3p from Saccharomyces cerevisiae
J. Biol. Chem., March 16, 2007; 282(11): 7862 - 7868.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Singh, S. Severance, N. Kaur, W. Wiltsie, and D. J. Kosman
Assembly, Activation, and Trafficking of the Fet3p{middle dot}Ftr1p High Affinity Iron Permease Complex in Saccharomyces cerevisiae
J. Biol. Chem., May 12, 2006; 281(19): 13355 - 13364.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. Lesuisse, S. A. B. Knight, M. Courel, R. Santos, J.-M. Camadro, and A. Dancis
Genome-Wide Screen for Genes With Effects on Distinct Iron Uptake Activities in Saccharomyces cerevisiae
Genetics, January 1, 2005; 169(1): 107 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Serrano, D. Bernal, E. Simon, and J. Arino
Copper and Iron Are the Limiting Factors for Growth of the Yeast Saccharomyces cerevisiae in an Alkaline Environment
J. Biol. Chem., May 7, 2004; 279(19): 19698 - 19704.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Shi, C. Stoj, A. Romeo, D. J. Kosman, and Z. Zhu
Fre1p Cu2+ Reduction and Fet3p Cu1+ Oxidation Modulate Copper Toxicity in Saccharomyces cerevisiae
J. Biol. Chem., December 12, 2003; 278(50): 50309 - 50315.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. T. Jensen, M. Ajua-Alemanji, and V. C. Culotta
The Saccharomyces cerevisiae High Affinity Phosphate Transporter Encoded by PHO84 Also Functions in Manganese Homeostasis
J. Biol. Chem., October 24, 2003; 278(43): 42036 - 42040.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Pelletier, J. Beaudoin, C. C. Philpott, and S. Labbe
Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system
Nucleic Acids Res., August 1, 2003; 31(15): 4332 - 4344.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
H.-J. Kim, H.-M. Kim, J.-H. Kim, K.-S. Ryu, S.-M. Park, K.-Y. Jahng, M.-S. Yang, and D.-H. Kim
Expression of Heteropolymeric Ferritin Improves Iron Storage in Saccharomyces cerevisiae
Appl. Envir. Microbiol., April 1, 2003; 69(4): 1999 - 2005.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Herbik, C. Bolling, and T. J. Buckhout
The Involvement of a Multicopper Oxidase in Iron Uptake by the Green Algae Chlamydomonas reinhardtii
Plant Physiology, December 1, 2002; 130(4): 2039 - 2048.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. La Fontaine, J. M. Quinn, S. S. Nakamoto, M. D. Page, V. Gohre, J. L. Moseley, J. Kropat, and S. Merchant
Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii
Eukaryot. Cell, October 1, 2002; 1(5): 736 - 757.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. M. Waters and D. J. Eide
Combinatorial Control of Yeast FET4 Gene Expression by Iron, Zinc, and Oxygen
J. Biol. Chem., September 6, 2002; 277(37): 33749 - 33757.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Pelletier, J. Beaudoin, Y. Mukai, and S. Labbe
Fep1, an Iron Sensor Regulating Iron Transporter Gene Expression in Schizosaccharomyces pombe
J. Biol. Chem., June 14, 2002; 277(25): 22950 - 22958.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. De Freitas, A. Liba, R. Meneghini, J. S. Valentine, and E. B. Gralla
Yeast Lacking Cu-Zn Superoxide Dismutase Show Altered Iron Homeostasis. ROLE OF OXIDATIVE STRESS IN IRON METABOLISM
J. Biol. Chem., April 14, 2000; 275(16): 11645 - 11649.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Urbanowski and R. C. Piper
The Iron Transporter Fth1p Forms a Complex with the Fet5 Iron Oxidase and Resides on the Vacuolar Membrane
J. Biol. Chem., December 31, 1999; 274(53): 38061 - 38070.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. M. O. Peña, J. Lee, and D. J. Thiele
A Delicate Balance: Homeostatic Control of Copper Uptake and Distribution
J. Nutr., July 1, 1999; 129(7): 1251 - 1260.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. Radisky and J. Kaplan
Regulation of Transition Metal Transport across the Yeast Plasma Membrane
J. Biol. Chem., February 19, 1999; 274(8): 4481 - 4484.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. S. Gitan, H. Luo, J. Rodgers, M. Broderius, and D. Eide
Zinc-induced Inactivation of the Yeast ZRT1 Zinc Transporter Occurs through Endocytosis and Vacuolar Degradation
J. Biol. Chem., October 30, 1998; 273(44): 28617 - 28624.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Li and J. Kaplan
Defects in the Yeast High Affinity Iron Transport System Result in Increased Metal Sensitivity because of the Increased Expression of Transporters with a Broad Transition Metal Specificity
J. Biol. Chem., August 28, 1998; 273(35): 22181 - 22187.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yu and M. Wessling-Resnick
Influence of Copper Depletion on Iron Uptake Mediated by SFT, a Stimulator of Fe Transport
J. Biol. Chem., March 20, 1998; 273(12): 6909 - 6915.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Gross, M. Kelleher, V. R. Iyer, P. O. Brown, and D. R. Winge
Identification of the Copper Regulon in Saccharomyces cerevisiae by DNA Microarrays
J. Biol. Chem., October 6, 2000; 275(41): 32310 - 32316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P.-L. Blaiseau, E. Lesuisse, and J.-M. Camadro
Aft2p, a Novel Iron-regulated Transcription Activator That Modulates, with Aft1p, Intracellular Iron Use and Resistance to Oxidative Stress in Yeast
J. Biol. Chem., August 31, 2001; 276(36): 34221 - 34226.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.