HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gorbunov, N. V. Articles by Kagan, V. E. Search for Related Content PubMed PubMed Citation Articles by Gorbunov, N. V. Articles by Kagan, V. E. Social Bookmarking                      What's this?

Volume 272, Number 19, Issue of May 9, 1997 pp. 12328-12341
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Nitric Oxide Prevents Oxidative Damage Produced by tert-Butyl Hydroperoxide in Erythroleukemia Cells via Nitrosylation of Heme and Non-heme Iron
ELECTRON PARAMAGNETIC RESONANCE EVIDENCE

(Received for publication, June 28, 1996, and in revised form, February 26, 1997)

Nikolai V. Gorbunov ^§ , Jack C. Yalowich ^** , Arunasri Gaddam ^§ , Padmakumari Thampatty ^** , Vladimir B. Ritov ^§ , Elena R. Kisin ^§ , Nabil M. Elsayed and Valerian E. Kagan ^§**

From the Departments of ^§ Environmental and Occupational Health and  Pharmacology, University of Pittsburgh, and ^** Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15238, and the  Department of Respiratory Research, Division of Medicine, Walter Reed Army Institute of Research, Washington, D. C. 20307

We studied protective effects of NO against tert-butylhydroperoxide (t-BuOOH)-induced oxidations in a subline of human erythroleukemia K562 cells with different intracellular hemoglobin (Hb) concentrations. t-BuOOH-induced formation of oxoferryl-Hb-derived free radical species in cells was demonstrated by low temperature EPR spectroscopy. Intensity of the signals was proportional to Hb concentrations and was correlated with cell viability. Peroxidation of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and cardiolipin metabolically labeled with oxidation-sensitive cis-parinaric acid was induced by t-BuOOH. An NO donor, (Z)-1-[N-(3-ammoniopropyl)-N-(n-propyl)amino]-diazen-1-ium-1,2-diolate], produced non-heme iron dinitrosyl complexes and hexa- and pentacoordinated Hb-nitrosyl complexes in the cells. Nitrosylation of non-heme iron centers and Hb-heme protected against t-BuOOH-induced: (a) formation of oxoferryl-Hb-derived free radical species, (b) peroxidation of cis-parinaric acid-labeled phospholipids, and (c) cytotoxicity. Since NO did not inhibit peroxidation induced by an azo-initiator of peroxyl radicals, 2,2-azobis(2,4-dimethylvaleronitrile), protective effects of NO were due to formation of iron-nitrosyl complexes whose redox interactions with t-BuOOH prevented generation of oxoferryl-Hb-derived free radical species.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Husain, T. J. Bourret, B. D. McCollister, J. Jones-Carson, J. Laughlin, and A. Vazquez-Torres Nitric Oxide Evokes an Adaptive Response to Oxidative Stress by Arresting Respiration J. Biol. Chem., March 21, 2008; 283(12): 7682 - 7689. [Abstract] [Full Text] [PDF]

				A. Diet, K. Abbas, C. Bouton, B. Guillon, F. Tomasello, S. Fourquet, M. B. Toledano, and J.-C. Drapier Regulation of Peroxiredoxins by Nitric Oxide in Immunostimulated Macrophages J. Biol. Chem., December 14, 2007; 282(50): 36199 - 36205. [Abstract] [Full Text] [PDF]

				C.-C. Hong, C. B. Ambrosone, J. Ahn, J.-Y. Choi, M. L. McCullough, V. L. Stevens, C. Rodriguez, M. J. Thun, and E. E. Calle Genetic Variability in Iron-Related Oxidative Stress Pathways (Nrf2, NQ01, NOS3, and HO-1), Iron Intake, and Risk of Postmenopausal Breast Cancer Cancer Epidemiol. Biomarkers Prev., September 1, 2007; 16(9): 1784 - 1794. [Abstract] [Full Text] [PDF]

				J. L. Atkins, B. W. Day, M. T. Handrigan, Z. Zhang, M. B. Pamnani, and N. V. Gorbunov Brisk production of nitric oxide and associated formation of S-nitrosothiols in early hemorrhage J Appl Physiol, April 1, 2006; 100(4): 1267 - 1277. [Abstract] [Full Text] [PDF]

				I. Gusarov and E. Nudler NO-mediated cytoprotection: Instant adaptation to oxidative stress in bacteria PNAS, September 27, 2005; 102(39): 13855 - 13860. [Abstract] [Full Text] [PDF]

				F. Feger, H. Ferry-Dumazet, M. M. Matsuda, J. Bordenave, M. Dupouy, A. K. Nussler, M. Arock, L. Devevey, J. Nafziger, J.-J. Guillosson, et al. Role of Iron in Tumor Cell Protection from the Pro-Apoptotic Effect of Nitric Oxide Cancer Res., July 1, 2001; 61(13): 5289 - 5294. [Abstract] [Full Text] [PDF]

				A. L. Kleschyov, B. Muller, T. Keravis, M.-E. Stoeckel, and J.-C. Stoclet Adventitia-derived nitric oxide in rat aortas exposed to endotoxin: cell origin and functional consequences Am J Physiol Heart Circ Physiol, December 1, 2000; 279(6): H2743 - H2751. [Abstract] [Full Text] [PDF]

				D. W Kamp and S. A Weitzman The molecular basis of asbestos induced lung injury Thorax, July 1, 1999; 54(7): 638 - 652. [Full Text]

				M. Juckett, Y. Zheng, H. Yuan, T. Pastor, W. Antholine, M. Weber, and G. Vercellotti Heme and the Endothelium. EFFECTS OF NITRIC OXIDE ON CATALYTIC IRON AND HEME DEGRADATION BY HEME OXYGENASE J. Biol. Chem., September 4, 1998; 273(36): 23388 - 23397. [Abstract] [Full Text] [PDF]