HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bogdanov, Y. D. Articles by Burnstock, G. Search for Related Content PubMed PubMed Citation Articles by Bogdanov, Y. D. Articles by Burnstock, G. Social Bookmarking                      What's this?

Volume 272, Number 19, Issue of May 9, 1997 pp. 12583-12590
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Early Expression of a Novel Nucleotide Receptor in the Neural Plate of Xenopus Embryos

(Received for publication, November 27, 1996, and in revised form, February 5, 1997)

From the Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom

Extracellular ATP functions as a neurotransmitter and neuromodulator in the adult nervous system, and a signaling molecule in non-neural tissue, acting either via ligand-gated ion channels (P2X) or G-protein-coupled receptors (P2Y). ATP can cause an increase in intracellular Ca²⁺ (Ca²⁺_i) in embryonic cells and so regulate cell proliferation, migration, and differentiation. We have isolated a Xenopus cDNA encoding a novel P2Y receptor, XlP2Y, which is expressed abundantly in developing embryos. Recombinant XlP2Y responds equally to all five naturally occurring nucleoside triphosphates (ATP, UTP, CTP, GTP, and ITP), which elicit a biphasic Ca²⁺-dependent Cl current (I_Cl,Ca) where the second phase persists for up to 60 min. XlP2Y also causes a continuous release of Ca²⁺_i and a low level persistent activation of I_Cl,Ca in Xenopus oocytes through the spontaneous efflux of ATP. mRNAs for XlP2Y are expressed transiently in the neural plate and tailbud during Xenopus development, coincident with neurogenesis. This restricted pattern of expression and novel pharmacological features confer unique properties to XlP2Y, which may play a key role in the early development of neural tissue.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Dale Dynamic ATP signalling and neural development J. Physiol., May 15, 2008; 586(10): 2429 - 2436. [Abstract] [Full Text] [PDF]

				M. Sundqvist and S. Holmgren Changes in the control of gastric motor activity during metamorphosis in the amphibian Xenopus laevis, with special emphasis on purinergic mechanisms J. Exp. Biol., April 15, 2008; 211(8): 1270 - 1280. [Abstract] [Full Text] [PDF]

				M. Sundqvist Developmental changes of purinergic control of intestinal motor activity during metamorphosis in the African clawed frog, Xenopus laevis Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1916 - R1925. [Abstract] [Full Text] [PDF]

				G. Burnstock Physiology and Pathophysiology of Purinergic Neurotransmission Physiol Rev, April 1, 2007; 87(2): 659 - 797. [Abstract] [Full Text] [PDF]

				J. S. Heo and H. J. Han ATP Stimulates Mouse Embryonic Stem Cell Proliferation via Protein Kinase C, Phosphatidylinositol 3-Kinase/Akt, and Mitogen-Activated Protein Kinase Signaling Pathways Stem Cells, December 1, 2006; 24(12): 2637 - 2648. [Abstract] [Full Text] [PDF]

				M. P. Abbracchio, G. Burnstock, J.-M. Boeynaems, E. A. Barnard, J. L. Boyer, C. Kennedy, G. E. Knight, M. Fumagalli, C. Gachet, K. A. Jacobson, et al. International Union of Pharmacology LVIII: Update on the P2Y G Protein-Coupled Nucleotide Receptors: From Molecular Mechanisms and Pathophysiology to Therapy Pharmacol. Rev., September 1, 2006; 58(3): 281 - 341. [Abstract] [Full Text] [PDF]

				M. D. Tran and J. T. Neary Purinergic signaling induces thrombospondin-1 expression in astrocytes PNAS, June 13, 2006; 103(24): 9321 - 9326. [Abstract] [Full Text] [PDF]

				K. Sak, J.-M. Boeynaems, and H. Everaus Involvement of P2Y receptors in the differentiation of haematopoietic cells J. Leukoc. Biol., April 1, 2003; 73(4): 442 - 447. [Abstract] [Full Text] [PDF]

				R. O. Arellano, A. Martinez-Torres, and E. Garay Ionic Currents Activated via Purinergic Receptors in the Cumulus Cell-Enclosed Mouse Oocyte Biol Reprod, September 1, 2002; 67(3): 837 - 846. [Abstract] [Full Text] [PDF]

				P. Brown and N. Dale Modulation of K+ currents in Xenopus spinal neurons by p2y receptors: a role for ATP and ADP in motor pattern generation J. Physiol., May 1, 2002; 540(3): 843 - 850. [Abstract] [Full Text] [PDF]

				R. A. Nicholas Identification of the P2Y12 Receptor: A Novel Member of the P2Y Family of Receptors Activated by Extracellular Nucleotides Mol. Pharmacol., September 1, 2001; 60(3): 416 - 420. [Full Text] [PDF]

				C. Kennedy, A.-D. Qi, C. L. Herold, T. K. Harden, and R. A. Nicholas ATP, an Agonist at the Rat P2Y4 Receptor, Is an Antagonist at the Human P2Y4 Receptor Mol. Pharmacol., May 1, 2000; 57(5): 926 - 931. [Abstract] [Full Text]

				J. L. Boyer, S. M. Delaney, D. Villanueva, and T. K. Harden A Molecularly Identified P2Y Receptor Simultaneously Activates Phospholipase C and Inhibits Adenylyl Cyclase and Is Nonselectively Activated by All Nucleoside Triphosphates Mol. Pharmacol., April 1, 2000; 57(4): 805 - 810. [Abstract] [Full Text]

				S. Rao, L. A. Garrett-Sinha, J. Yoon, and M. C. Simon The Ets Factors PU.1 and Spi-B Regulate the Transcription in Vivo of P2Y10, a Lymphoid Restricted Heptahelical Receptor J. Biol. Chem., November 26, 1999; 274(48): 34245 - 34252. [Abstract] [Full Text] [PDF]

				A. J. Morris and C. C. Malbon Physiological Regulation of G Protein-Linked Signaling Physiol Rev, October 1, 1999; 79(4): 1373 - 1430. [Abstract] [Full Text] [PDF]

				D. E. McCoy, A. L. Taylor, B. A. Kudlow, K. Karlson, M. J. Slattery, L. M. Schwiebert, E. M. Schwiebert, and B. A. Stanton Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors Am J Physiol Renal Physiol, October 1, 1999; 277(4): F552 - F559. [Abstract] [Full Text] [PDF]

				J.T. Turner, L.A. landon, S.J. Gibbons, and B.R. Talamo Salivary Gland P2 Nucleotide Receptors Critical Reviews in Oral Biology & Medicine, January 1, 1999; 10(2): 210 - 224. [Abstract] [Full Text] [PDF]

				V. Ralevic and G. Burnstock Receptors for Purines and Pyrimidines Pharmacol. Rev., September 1, 1998; 50(3): 413 - 492. [Abstract] [Full Text] [PDF]

				D. Communi, C. Govaerts, M. Parmentier, and J.-M. Boeynaems Cloning of a Human Purinergic P2Y Receptor Coupled to Phospholipase C and Adenylyl Cyclase J. Biol. Chem., December 19, 1997; 272(51): 31969 - 31973. [Abstract] [Full Text] [PDF]

				J. L. Boyer, G. L. Waldo, and T. K. Harden Molecular Cloning and Expression of an Avian G Protein-Coupled P2Y Receptor Mol. Pharmacol., December 1, 1997; 52(6): 928 - 934. [Abstract] [Full Text]

				J. A. Dranoff, A. F. O'Neill, A. M. Franco, S.-Y. Cai, G. C. Connolly, N. Ballatori, J. L. Boyer, and M. H. Nathanson A Primitive ATP Receptor from the Little Skate Raja erinacea J. Biol. Chem., September 22, 2000; 275(39): 30701 - 30706. [Abstract] [Full Text] [PDF]

				D. Communi, N. S. Gonzalez, M. Detheux, S. Brezillon, V. Lannoy, M. Parmentier, and J.-M. Boeynaems Identification of a Novel Human ADP Receptor Coupled to Gi J. Biol. Chem., October 26, 2001; 276(44): 41479 - 41485. [Abstract] [Full Text] [PDF]

				P. Brown and N. Dale Modulation of K+ currents in Xenopus spinal neurons by p2y receptors: a role for ATP and ADP in motor pattern generation J. Physiol., March 28, 2002; (2002) 2001013192. [Abstract] [PDF]