HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Halldén, G. Articles by Aponte, G. W. Search for Related Content PubMed PubMed Citation Articles by Halldén, G. Articles by Aponte, G. W. Social Bookmarking                      What's this?

Volume 272, Number 19, Issue of May 9, 1997 pp. 12591-12600
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Evidence for a Role of the Gut Hormone PYY in the Regulation of Intestinal Fatty Acid-binding Protein Transcripts in Differentiated Subpopulations of Intestinal Epithelial Cell Hybrids

(Received for publication, December 17, 1996, and in revised form, February 12, 1997)

From the Department of Nutritional Sciences, University of California, Berkeley, California 94720-3104

Peptide tyrosine tyrosine (PYY) is a gut hormone present in endocrine cells in the lower intestine that can be released by the presence of luminal free fatty acids (FFAs). The biological action of this peptide includes inhibition of gut motility and gastrointestinal and pancreatic secretions. Intestinal fatty acid-binding protein (I-FABP) binds FFA and may be involved in their cytosolic trafficking. Quantitative in situ hybridization on heterogeneous populations of small intestinal somatic cell hybrids selected for endogenous I-FABP expression (hBRIE 380i cells) demonstrated a 5-fold increase in I-FABP transcripts in response to PYY (within 6 h) that was confined to clusters of differentiated cells, whereas ribonuclease protection assays performed on heterogeneous populations of these cells showed no significant differences. High affinity PYY receptors, with an IC₅₀ of 5-50 pM, were identified in both differentiated and nondifferentiated cell populations, as determined by competitive binding assays and autoradiography. In situ hybridization of rat ileal tissue also revealed differing patterns of mRNA expression for liver fatty acid-binding protein (L-FABP) and I-FABP. Only I-FABP mRNA was detected in the villus tips. This localization correlated with the expression pattern of I-FABP mRNA in the hBRIE 380i cells where changes in transcripts were observed only in differentiated cells that did not incorporate bromodeoxyuridine. The sustained expression of I-FABP transcripts in the villar tips suggests (unlike L-FABP) that older terminally differentiated cell populations of the mucosa can still be PYY responsive. These studies demonstrate that physiological concentrations of PYY can regulate I-FABP and place this peptide in a key position as part of a feedback system that determines the processing of cytosolic FFA in the enterocyte. In addition, these studies suggest a mechanism whereby luminal agents can modulate expression of proteins in terminally differentiated cells in the gastrointestinal mucosa.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Choi, M. Lee, A. L. Shiu, S. J. Yo, and G. W. Aponte Identification of a protein hydrolysate responsive G protein-coupled receptor in enterocytes Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G98 - G112. [Abstract] [Full Text] [PDF]

				M. Lee, M. Hadi, G. Hallden, and G. W. Aponte Peptide YY and Neuropeptide Y Induce Villin Expression, Reduce Adhesion, and Enhance Migration in Small Intestinal Cells through the Regulation of CD63, Matrix Metalloproteinase-3, and Cdc42 Activity J. Biol. Chem., January 7, 2005; 280(1): 125 - 136. [Abstract] [Full Text] [PDF]

				J. T. Mahan, G. D. Heda, R. H. Rao, and C. M. Mansbach II The intestine expresses pancreatic triacylglycerol lipase: regulation by dietary lipid Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1187 - G1196. [Abstract] [Full Text] [PDF]

				K. Sonoyama, K. Suzuki, and T. Kasai Peptide YY Stimulates the Expression of Apolipoprotein A-IV Gene in Caco-2 Intestinal Cells Experimental Biology and Medicine, March 1, 2000; 223(3): 270 - 275. [Abstract] [Full Text]

				C. Darimont, N. Gradoux, E. Persohn, F. Cumin, and A. De Pover Effects of intestinal fatty acid-binding protein overexpression on fatty acid metabolism in Caco-2 cells J. Lipid Res., January 1, 2000; 41(1): 84 - 92. [Abstract] [Full Text]

				G. Hallden, M. Hadi, H. T. Hong, and G. W. Aponte Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells J. Biol. Chem., September 24, 1999; 274(39): 27914 - 27924. [Abstract] [Full Text] [PDF]

				C. Darimont, N. Gradoux, and A. de Pover Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G606 - G612. [Abstract] [Full Text] [PDF]

				T. J. Kalogeris, X. Qin, W. Y. Chey, and P. Tso PYY stimulates synthesis and secretion of intestinal apolipoprotein AIV without affecting mRNA expression Am J Physiol Gastrointest Liver Physiol, October 1, 1998; 275(4): G668 - G674. [Abstract] [Full Text] [PDF]