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(Received for publication, October 9, 1996, and in revised form, February 10, 1997)
From the Departments of We have examined the expression of the small
extracellular chondroitin/dermatan sulfate proteoglycans (CS/DS PGs),
biglycan, decorin, and PG-100, which is the proteoglycan form of colony stimulating factor-1, in the human endothelial cell line EA.hy 926. We
have also examined whether modulation of the phenotype of EA.hy 926 cells by tumor necrosis factor-
Volume 272, Number 19,
Issue of May 9, 1997
pp. 12730-12737
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
,
,**
Medical Biochemistry and
** Internal Medicine,
Department of Anatomy, University of Kuopio, FIN-70211
Kuopio, Finland
(TNF-) is associated with
specific changes in the synthesis of these PGs. We demonstrate that
EA.hy 926 cells, when they form monolayer cultures typical of
macrovascular endothelial cells, express and synthesize detectable amounts of biglycan and PG-100, but not decorin. On SDS-polyacrylamide gel electrophoresis both PGs behave like proteins of the relative molecular weight of ~250,000. TNF- that changed the morphology of
the cells from a polygonal shape into a spindle shape and that also
stimulated the detachment of the cells from culture dish, markedly
decreased the net synthesis of biglycan, whereas the net synthesis of
PG-100 was increased. These changes were parallel with those observed
at the mRNA level of the corresponding PGs. The proportions of the
different sulfated CS/DS disaccharide units of PGs were not affected by
TNF-. Several other growth factors/cytokines, such as
interferon-
, fibroblast growth factors-2 (FGF-2) and -7 (FGF-7),
interleukin-1
, and transforming growth factor-, unlike TNF-,
modulated neither the morphology nor the biglycan expression of EA.hy
926 cells under the conditions used in the experiments. However, PG-100
expression was increased also in response to FGF-2 and -7 and
transforming growth factor-. None of the above cytokines, including
TNF-, was able to induce decorin expression in the cells. Our
results indicate that the regulatory elements controlling the
expression of the small extracellular CS/DS PGs in human endothelial
cells are different.
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