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Volume 272, Number 20, Issue of May 16, 1997 pp. 12928-12937
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Human TEF-5 Is Preferentially Expressed in Placenta and Binds to Multiple Functional Elements of the Human Chorionic Somatomammotropin-B Gene Enhancer

(Received for publication, October 22, 1996, and in revised form, January 30, 1997)

Patrick Jacquemin ^§ , Joseph A. Martial ^§ and Irwin Davidson

From the  Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, B.P. 163-67404 Illkirch Cédex, France and the ^§ Laboratoire de Biologie Moléculaire et de Génie Génétique, Institut de Chimie-B6, Université de Liège, B-4000 Sart-Tilman, Belgium

We report the cloning of a cDNA encoding the human transcription factor hTEF-5, containing the TEA/ATTS DNA binding domain and related to the TEF family of transcription factors. hTEF-5 is expressed in skeletal and cardiac muscle, but the strongest expression is observed in the placenta and in placenta-derived JEG-3 choriocarcinoma cells. In correlation with its placental expression, we show that hTEF-5 binds to several functional enhansons of the human chorionic somatomammotropin (hCS)-B gene enhancer. We define a novel functional element in this enhancer comprising tandemly repeated sites to which hTEF-5 binds cooperatively. In the corresponding region of the hCS-A enhancer, which is known to be inactive, this element is inactivated by a naturally occurring single base mutation that disrupts hTEF-5 binding. We further show that the binding of the previously described placental protein f/chorionic somatomammotropin enhancer factor-1 to TEF-binding sites is disrupted by monoclonal antibodies directed against the TEA domain and that this factor is a proteolytic degradation product of the TEF factors. These results strongly suggest that hTEF-5 regulates the activity of the hCS-B gene enhancer.

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