HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marinelli, R. A. Articles by LaRusso, N. F. Search for Related Content PubMed PubMed Citation Articles by Marinelli, R. A. Articles by LaRusso, N. F. Social Bookmarking                      What's this?

Volume 272, Number 20, Issue of May 16, 1997 pp. 12984-12988
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Secretin Promotes Osmotic Water Transport in Rat Cholangiocytes by Increasing Aquaporin-1 Water Channels in Plasma Membrane
EVIDENCE FOR A SECRETIN-INDUCED VESICULAR TRANSLOCATION OF AQUAPORIN-1

(Received for publication, December 24, 1996, and in revised form, March 6, 1997)

Raul A. Marinelli , Linh Pham , Peter Agre ^§ and Nicholas F. LaRusso

From the  Center for Basic Research in Digestive Diseases, Departments of Internal Medicine, Biochemistry, and Molecular Biology, Mayo Clinic and Foundation, Mayo Medical School, Rochester, Minnesota 55905 and the ^§ Departments of Biological Chemistry and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Although secretin is known to stimulate ductal bile secretion by directly interacting with cholangiocytes, the precise cellular mechanisms accounting for this choleretic effect are unknown. We have previously shown that secretin stimulates exocytosis in cholangiocytes and that these cells transport water mainly via the water channel aquaporin-1 (AQP1). In this study, we tested the hypothesis that secretin promotes osmotic water movement in cholangiocytes by inducing the exocytic insertion of AQP1 into plasma membranes. Exposure of highly purified isolated rat cholangiocytes to secretin caused significant, dose-dependent increases in osmotic membrane water permeability (P_f) (e.g. increased by 60% with 10⁷ M secretin), which was reversibly inhibited by the water channel blocker HgCl₂. Immunoblotting analysis of cholangiocyte membrane fractions showed that secretin caused up to a 3-fold increase in the amount of AQP1 in plasma membranes and a proportional decrease in the amount of the water channel in microsomes, suggesting a secretin-induced redistribution of AQP1 from intracellular to plasma membranes. Both the secretin-induced increase in cholangiocyte P_f and AQP1 redistribution were blocked by two perturbations that inhibit secretin-stimulated exocytosis in cholangiocytes, i.e. treatment with colchicine and exposure at low temperatures (20 and 4 °C). Our results demonstrate that secretin increases AQP1-mediated P_f in cholangiocytes. Moreover, our studies implicate the microtubule-dependent vesicular translocation of AQP1 water channels to the plasma membrane, a mechanism that appears to be essential for secretin-induced ductal bile secretion and suggests that AQP1 can be regulated by membrane trafficking.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Li, H. Zhang, T. Ma, and A. S. Verkman Very high aquaporin-1 facilitated water permeability in mouse gallbladder Am J Physiol Gastrointest Liver Physiol, April 1, 2009; 296(4): G816 - G822. [Abstract] [Full Text] [PDF]

				J. Y. S. Chu, S. C. K. Chung, A. K. M. Lam, S. Tam, S. K. Chung, and B. K. C. Chow Phenotypes Developed in Secretin Receptor-Null Mice Indicated a Role for Secretin in Regulating Renal Water Reabsorption Mol. Cell. Biol., April 1, 2007; 27(7): 2499 - 2511. [Abstract] [Full Text] [PDF]

				E.-J. Kamsteeg, G. Hendriks, M. Boone, I. B. M. Konings, V. Oorschot, P. van der Sluijs, J. Klumperman, and P. M. T. Deen Short-chain ubiquitination mediates the regulated endocytosis of the aquaporin-2 water channel PNAS, November 28, 2006; 103(48): 18344 - 18349. [Abstract] [Full Text] [PDF]

				Y. Ishikawa, Z. Yuan, N. Inoue, M. T. Skowronski, Y. Nakae, M. Shono, G. Cho, M. Yasui, P. Agre, and S. Nielsen Identification of AQP5 in lipid rafts and its translocation to apical membranes by activation of M3 mAChRs in interlobular ducts of rat parotid gland Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1303 - C1311. [Abstract] [Full Text] [PDF]

				X.-M. Chen, S. P. O'Hara, B. Q. Huang, P. L. Splinter, J. B. Nelson, and N. F. LaRusso Localized glucose and water influx facilitates Cryptosporidium parvum cellular invasion by means of modulation of host-cell membrane protrusion PNAS, May 3, 2005; 102(18): 6338 - 6343. [Abstract] [Full Text] [PDF]

				A. D. Mottino, F. A. Crocenzi, E. J. S. Pozzi, L. M. Veggi, M. G. Roma, and M. Vore Role of microtubules in estradiol-17{beta}-D-glucuronide-induced alteration of canalicular Mrp2 localization and activity Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G327 - G336. [Abstract] [Full Text] [PDF]

				R. J. Davis, K. J. Page, G. J. Dos Santos Cruz, D. W. Harmer, P. W. Munday, S. J. Williams, J. Picot, T. J. Evans, R. L. Sheldrick, R. A. Coleman, et al. Expression and Functions of the Duodenal Peptide Secretin and its Receptor in Human Lung Am. J. Respir. Cell Mol. Biol., September 1, 2004; 31(3): 302 - 308. [Abstract] [Full Text] [PDF]

				F. A. Crocenzi, A. D. Mottino, J. Cao, L. M. Veggi, E. J. S. Pozzi, M. Vore, R. Coleman, and M. G. Roma Estradiol-17{beta}-D-glucuronide induces endocytic internalization of Bsep in rats Am J Physiol Gastrointest Liver Physiol, July 7, 2003; 285(2): G449 - G459. [Abstract] [Full Text] [PDF]

				B Burghardt, M-L Elkjaer, T-H Kwon, G Z Racz, G Varga, M C Steward, and S Nielsen Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas Gut, July 1, 2003; 52(7): 1008 - 1016. [Abstract] [Full Text]

				W. D. Stamer, D. Bok, J. Hu, G. J. Jaffe, and B. S. McKay Aquaporin-1 Channels in Human Retinal Pigment Epithelium: Role in Transepithelial Water Movement Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2803 - 2808. [Abstract] [Full Text] [PDF]

				A.-Y. Gong, P. S. Tietz, M. A. Muff, P. L. Splinter, R. C. Huebert, M. Z. Strowski, X.-M. Chen, and N. F. LaRusso Somatostatin stimulates ductal bile absorption and inhibits ductal bile secretion in mice via SSTR2 on cholangiocytes Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1205 - C1214. [Abstract] [Full Text] [PDF]

				P. L. Splinter, A. I. Masyuk, and N. F. LaRusso Specific Inhibition of AQP1 Water Channels in Isolated Rat Intrahepatic Bile Duct Units by Small Interfering RNAs J. Biol. Chem., February 14, 2003; 278(8): 6268 - 6274. [Abstract] [Full Text] [PDF]

				A. Mennone, A. S. Verkman, and J. L. Boyer Unimpaired osmotic water permeability and fluid secretion in bile duct epithelia of AQP1 null mice Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G739 - G746. [Abstract] [Full Text] [PDF]

				P. Agre, L. S King, M. Yasui, W. B Guggino, O. P. Ottersen, Y. Fujiyoshi, A. Engel, and S. Nielsen Aquaporin water channels - from atomic structure to clinical medicine J. Physiol., July 1, 2002; 542(1): 3 - 16. [Abstract] [Full Text] [PDF]

				A.-Y. Gong, A. I. Masyuk, P. L. Splinter, R. C. Huebert, P. S. Tietz, and N. F. LaRusso Channel-mediated water movement across enclosed or perfused mouse intrahepatic bile duct units Am J Physiol Cell Physiol, July 1, 2002; 283(1): C338 - C346. [Abstract] [Full Text] [PDF]

				R. C. Huebert, P. L. Splinter, F. Garcia, R. A. Marinelli, and N. F. LaRusso Expression and Localization of Aquaporin Water Channels in Rat Hepatocytes. EVIDENCE FOR A ROLE IN CANALICULAR BILE SECRETION J. Biol. Chem., June 14, 2002; 277(25): 22710 - 22717. [Abstract] [Full Text] [PDF]

				S.-J. Cho, A. K. M. A. Sattar, E.-H. Jeong, M. Satchi, J. A. Cho, S. Dash, M. S. Mayes, M. H. Stromer, and B. P. Jena Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles PNAS, March 21, 2002; (2002) 72083499. [Abstract] [Full Text] [PDF]

				N. Kanno, G. LeSage, S. Glaser, and G. Alpini Regulation of cholangiocyte bicarbonate secretion Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G612 - G625. [Abstract] [Full Text] [PDF]

				W. D. Stamer, K. Peppel, M. E. O'Donnell, B. C. Roberts, F. Wu, and D. L. Epstein Expression of Aquaporin-1 in Human Trabecular Meshwork Cells: Role in Resting Cell Volume Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1803 - 1811. [Abstract] [Full Text] [PDF]

				P. T. Hurley, C. J. Ferguson, T.-H. Kwon, M.-L. E. Andersen, A. G. Norman, M. C. Steward, S. Nielsen, and R. M. Case Expression and immunolocalization of aquaporin water channels in rat exocrine pancreas Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G701 - G709. [Abstract] [Full Text] [PDF]

				T. Ma, S. Jayaraman, K. S. Wang, Y. Song, B. Yang, J. Li, J. A. Bastidas, and A. S. Verkman Defective dietary fat processing in transgenic mice lacking aquaporin-1 water channels Am J Physiol Cell Physiol, January 1, 2001; 280(1): C126 - C134. [Abstract] [Full Text] [PDF]

				N. M. Sherwood, S. L. Krueckl, and J. E. McRory The Origin and Function of the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)/Glucagon Superfamily Endocr. Rev., December 1, 2000; 21(6): 619 - 670. [Abstract] [Full Text]

				R. M. Smith, B. Baibakov, Y. Ikebuchi, B. H. White, N. A. Lambert, L. K. Kaczmarek, and S. S. Vogel Exocytotic Insertion of Calcium Channels Constrains Compensatory Endocytosis to Sites of Exocytosis J. Cell Biol., February 21, 2000; 148(4): 755 - 768. [Abstract] [Full Text] [PDF]

				T. Ma and A S Verkman Aquaporin water channels in gastrointestinal physiology J. Physiol., June 1, 1999; 517(2): 317 - 326. [Abstract] [Full Text] [PDF]

				R. A. Marinelli, P. S. Tietz, L. D. Pham, L. Rueckert, P. Agre, and N. F. LaRusso Secretin induces the apical insertion of aquaporin-1 water channels in rat cholangiocytes Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G280 - G286. [Abstract] [Full Text] [PDF]

				K. Ishibashi and S. Sasaki The Dichotomy of MIP Family Suggests Two Separate Origins of Water Channels Physiology, June 1, 1998; 13(3): 137 - 142. [Abstract] [Full Text] [PDF]

				F. Garcia, A. Kierbel, M. C. Larocca, S. A. Gradilone, P. Splinter, N. F. LaRusso, and R. A. Marinelli The Water Channel Aquaporin-8 Is Mainly Intracellular in Rat Hepatocytes, and Its Plasma Membrane Insertion Is Stimulated by Cyclic AMP J. Biol. Chem., April 6, 2001; 276(15): 12147 - 12152. [Abstract] [Full Text] [PDF]

				S.-J. Cho, A. K. M. A. Sattar, E.-H. Jeong, M. Satchi, J. A. Cho, S. Dash, M. S. Mayes, M. H. Stromer, and B. P. Jena Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles PNAS, April 2, 2002; 99(7): 4720 - 4724. [Abstract] [Full Text] [PDF]

				R. B. Doctor, R. Dahl, L. Fouassier, G. Kilic, and J. G. Fitz Cholangiocytes exhibit dynamic, actin-dependent apical membrane turnover Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1042 - C1052. [Abstract] [Full Text] [PDF]

				P. M. T. Deen, B. W. M. Van Balkom, P. J. M. Savelkoul, E.-J. Kamsteeg, M. Van Raak, M. L. Jennings, T. R. Muth, V. Rajendran, and M. J. Caplan Aquaporin-2: COOH terminus is necessary but not sufficient for routing to the apical membrane Am J Physiol Renal Physiol, February 1, 2002; 282(2): F330 - F340. [Abstract] [Full Text] [PDF]