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(Received for publication, October 31, 1996, and in revised form, February 6, 1997)
From the Departments of Mycobacterium tuberculosis and the
closely related organism Mycobacterium bovis can survive
and replicate inside macrophages. Intracellular survival is at least in
part attributed to the failure of mycobacterial phagosomes to undergo
fusion with lysosomes. The transformation of phagosomes into
phagolysosomes involves gradual acquisition of markers from the
endosomal compartment. Members of the rab family of small GTPases
which confer fusion competence in the endocytic pathway are exchanged
sequentially onto the phagosomal membranes in the course of their
maturation. To identify the step at which the fusion capability of
phagosomes containing mycobacteria is compromised, we purified green
fluorescent protein-labeled M. bovis BCG phagosomal
compartments (MPC) and compared GTP-binding protein profiles of these
vesicles with latex bead phagosomal compartments (LBC). We report that
the MPC do not acquire rab7, specific for late endosomes, even 7 days
postinfection, whereas this GTP-binding protein is present on the LBC
within hours after phagocytosis. By contrast, rab5 is retained and
enriched with time on the MPC, suggesting fusion competence with an
early endosomal compartment. Prior infection of macrophages with
M. bovis BCG also affected the dynamics of rab5 and rab7
acquisition by subsequently formed LBC. Selective exclusion of rab7,
coupled with the retention of rab5 on the mycobacterial phagosome, may allow organisms from the M. tuberculosis complex to avert
the usual physiological destination of phagocytosed material.
Volume 272, Number 20,
Issue of May 16, 1997
pp. 13326-13331
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,
and
Microbiology and Immunology,
§ Ophthalmology and Anatomy and Cell Biology, University
of Michigan Medical School, Ann Arbor, Michigan 48109, the
¶ Department of Microbiology, University of Texas Health
Science Center, San Antonio, Texas 78284, and the 
Research
Institute of Molecular Pathology, A-1030 Vienna, Austria
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