HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Biswas, I. Articles by Hsieh, P. Search for Related Content PubMed PubMed Citation Articles by Biswas, I. Articles by Hsieh, P. Social Bookmarking                      What's this?

Volume 272, Number 20, Issue of May 16, 1997 pp. 13355-13364
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Interaction of MutS Protein with the Major and Minor Grooves of a Heteroduplex DNA

(Received for publication, January 10, 1997, and in revised form, March 12, 1997)

From the Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-1810

Thermus aquaticus MutS protein is a DNA mismatch repair protein that recognizes and binds to heteroduplex DNAs containing mispaired or unpaired bases. Using enzymatic and chemical probe methods, we have examined the binding of Taq MutS protein to a heteroduplex DNA having a single unpaired thymidine residue. DNase I footprinting identifies a symmetrical region of protection 24-28 nucleotides long centered on the unpaired base. Methylation protection and interference studies establish that Taq MutS protein makes contacts with the major groove of the heteroduplex in the immediate vicinity of the unpaired base. Hydroxyl radical and 1,10-phenanthroline-copper footprinting experiments indicate that MutS also interacts with the minor groove near the unpaired base. Together with the identification of key phosphate groups detected by ethylation interference, these data reveal critical contact points residing in the major and minor grooves of the heteroduplex DNA.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Biswas and I. Biswas Regulation of the Glucosyltransferase (gtfBC) Operon by CovR in Streptococcus mutans J. Bacteriol., February 1, 2006; 188(3): 988 - 998. [Abstract] [Full Text] [PDF]

				J. Kang, S. Huang, and M. J. Blaser Structural and Functional Divergence of MutS2 from Bacterial MutS1 and Eukaryotic MSH4-MSH5 Homologs J. Bacteriol., May 15, 2005; 187(10): 3528 - 3537. [Abstract] [Full Text] [PDF]

				L. Tsujikawa, M. Weinfield, and L. J. Reha-Krantz Differences in replication of a DNA template containing an ethyl phosphotriester by T4 DNA polymerase and Escherichia coli DNA polymerase I Nucleic Acids Res., September 1, 2003; 31(17): 4965 - 4972. [Abstract] [Full Text] [PDF]

				A. Yamamoto, M. J. Schofield, I. Biswas, and P. Hsieh Requirement for Phe36 for DNA binding and mismatch repair by Escherichia coli MutS protein Nucleic Acids Res., September 15, 2000; 28(18): 3564 - 3569. [Abstract] [Full Text] [PDF]

				P. Sachadyn, A. Stanisawska, and J. Kur One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA Nucleic Acids Res., April 15, 2000; 28(8): e36 - e. [Abstract] [Full Text] [PDF]

				G. T. Marsischky and R. D. Kolodner Biochemical Characterization of the Interaction between the Saccharomyces cerevisiae MSH2-MSH6 Complex and Mispaired Bases in DNA J. Biol. Chem., September 17, 1999; 274(38): 26668 - 26682. [Abstract] [Full Text] [PDF]

				E. D. Scheeff, J. M. Briggs, and S. B. Howell Molecular Modeling of the Intrastrand Guanine-Guanine DNA Adducts Produced by Cisplatin and Oxaliplatin Mol. Pharmacol., September 1, 1999; 56(3): 633 - 643. [Abstract] [Full Text]

				H. Terato, A. Masaoka, M. Kobayashi, S. Fukushima, Y. Ohyama, M. Yoshida, and H. Ide Enzymatic Repair of 5-Formyluracil. II. MISMATCH FORMATION BETWEEN 5-FORMYLURACIL AND GUANINE DURING DNA REPLICATION AND ITS RECOGNITION BY TWO PROTEINS INVOLVED IN BASE EXCISION REPAIR (AlkA) AND MISMATCH REPAIR (MutS) J. Biol. Chem., August 27, 1999; 274(35): 25144 - 25150. [Abstract] [Full Text] [PDF]

				I. Biswas, C. Ban, K. G. Fleming, J. Qin, J. W. Lary, D. A. Yphantis, W. Yang, and P. Hsieh Oligomerization of a MutS Mismatch Repair Protein from Thermus aquaticus J. Biol. Chem., August 13, 1999; 274(33): 23673 - 23678. [Abstract] [Full Text] [PDF]

				V. A. Malkov, I. Biswas, R. D. Camerini-Otero, and P. Hsieh Photocross-linking of the NH2-terminal Region of Taq MutS Protein to the Major Groove of a Heteroduplex DNA J. Biol. Chem., September 19, 1997; 272(38): 23811 - 23817. [Abstract] [Full Text] [PDF]

				M. J. Schofield, S. Nayak, T. H. Scott, C. Du, and P. Hsieh Interaction of Escherichia coli MutS and MutL at a DNA Mismatch J. Biol. Chem., July 20, 2001; 276(30): 28291 - 28299. [Abstract] [Full Text] [PDF]