| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, August 22, 1996, and in revised form, November 25, 1996)
From the The antibiotic lactacystin was reported to
covalently modify
Volume 272, Number 20,
Issue of May 16, 1997
pp. 13437-13445
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
-Lactone Modify
Multiple Proteasome -Subunits and Inhibit Intracellular Protein
Degradation and Major Histocompatibility Complex Class I Antigen
Presentation
,
,
Division of Lymphocyte Biology, Dana-Farber
Cancer Institute, Boston, Massachusetts 02115, the
§ Department of Cell Biology, Harvard Medical School,
Boston, Massachusetts 02115, the ¶ Department of Chemistry and
Chemical Biology, Harvard University, Cambridge, Massachusetts 02138,
and the 
Department of Pathology, Harvard Medical
School, Boston, Massachusetts 02115
-subunit X of the mammalian 20 S proteasome and
inhibit several of its peptidase activities. However, we demonstrate
that [3H]lactacystin treatment modifies all the
proteasome's catalytic -subunits. Lactacystin and its more potent
derivative -lactone irreversibly inhibit protein breakdown and the
chymotryptic, tryptic, and peptidylglutamyl activities of purified
20 S and 26 S particles, although at different rates. Exposure to
these agents for 1 to 2 h reduced the degradation of short- and
long-lived proteins in four different mammalian cell lines. Unlike
peptide aldehyde inhibitors, lactacystin and the -lactone do not
inhibit lysosomal degradation of an endocytosed protein. These agents
block class I antigen presentation of a model protein, ovalbumin
(synthesized endogenously or loaded exogenously), but do not affect
presentation of the peptide epitope SIINFEKL, which does not require
proteolysis for presentation. Generation of most peptides required for
formation of stable class I heterodimers is also inhibited. Because
these agents inhibited protein breakdown and antigen presentation
similarly in interferon-
-treated cells (where proteasomes contain
LMP2 and LMP7 subunits in place of X and Y), all -subunits must be affected similarly. These findings confirm our prior conclusions that
proteasomes catalyze the bulk of protein breakdown in mammalian cells
and generate the majority of class I-bound epitopes for immune
recognition.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Hedhli, P. Lizano, C. Hong, L. F. Fritzky, S. K. Dhar, H. Liu, Y. Tian, S. Gao, K. Madura, S. F. Vatner, et al. Proteasome inhibition decreases cardiac remodeling after initiation of pressure overload Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1385 - H1393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Johnstone, S. Guil, M. A. Rico, B. Garcia-Barreno, D. Lopez, J. A. Melero, and M. Del Val Relevance of viral context and diversity of antigen-processing routes for respiratory syncytial virus cytotoxic T-lymphocyte epitopes J. Gen. Virol., September 1, 2008; 89(9): 2194 - 2203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Weck, S. Appel, D. Werth, C. Sinzger, A. Bringmann, F. Grunebach, and P. Brossart hDectin-1 is involved in uptake and cross-presentation of cellular antigens Blood, April 15, 2008; 111(8): 4264 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Seiliez, S. Panserat, S. Skiba-Cassy, A. Fricot, C. Vachot, S. Kaushik, and S. Tesseraud Feeding Status Regulates the Polyubiquitination Step of the Ubiquitin-Proteasome-Dependent Proteolysis in Rainbow Trout (Oncorhynchus mykiss) Muscle J. Nutr., March 1, 2008; 138(3): 487 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Didier, A. Merdes, J.-E. Gairin, and N. Jabrane-Ferrat Inhibition of Proteasome Activity Impairs Centrosome-dependent Microtubule Nucleation and Organization Mol. Biol. Cell, March 1, 2008; 19(3): 1220 - 1229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hedhli, L. Wang, Q. Wang, E. Rashed, Y. Tian, X. Sui, K. Madura, and C. Depre Proteasome activation during cardiac hypertrophy by the chaperone H11 Kinase/Hsp22 Cardiovasc Res, February 1, 2008; 77(3): 497 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shi, G. J. Tricot, T. K. Garg, P. A. Malaviarachchi, S. M. Szmania, R. E. Kellum, B. Storrie, A. Mulder, J. D. Shaughnessy Jr, B. Barlogie, et al. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma Blood, February 1, 2008; 111(3): 1309 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Granot, O. Kobiler, N. Melamed-Book, S. Eimerl, A. Bahat, B. Lu, S. Braun, M. R. Maurizi, C. K. Suzuki, A. B. Oppenheim, et al. Turnover of Mitochondrial Steroidogenic Acute Regulatory (StAR) Protein by Lon Protease: The Unexpected Effect of Proteasome Inhibitors Mol. Endocrinol., September 1, 2007; 21(9): 2164 - 2177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. H. van Hees, H. F. M. van der Heijden, C. A. C. Ottenheijm, L. M. A. Heunks, C. J. C. Pigmans, F. W. A. Verheugt, R. M. H. J. Brouwer, and P. N. R. Dekhuijzen Diaphragm single-fiber weakness and loss of myosin in congestive heart failure rats Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H819 - H828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Jin, W. Ding, and K. M. Mulder Requirement for the Dynein Light Chain km23-1 in a Smad2-dependent Transforming Growth Factor-beta Signaling Pathway J. Biol. Chem., June 29, 2007; 282(26): 19122 - 19132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guil, M. Rodriguez-Castro, F. Aguilar, E. M. Villasevil, L. C. Anton, and M. Del Val Need for Tripeptidyl-peptidase II in Major Histocompatibility Complex Class I Viral Antigen Processing when Proteasomes are Detrimental J. Biol. Chem., December 29, 2006; 281(52): 39925 - 39934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Walton, M. Gerlinger, O. de la Rosa, N. Nuber, A. Knights, A. Gati, M. Laumer, L. Strauss, C. Exner, N. Schafer, et al. Spontaneous CD8 T Cell Responses against the Melanocyte Differentiation Antigen RAB38/NY-MEL-1 in Melanoma Patients J. Immunol., December 1, 2006; 177(11): 8212 - 8218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Caudill, K. Jayarapu, L. Elenich, J. J. Monaco, R. A. Colbert, and T. A. Griffin T Cells Lacking Immunoproteasome Subunits MECL-1 and LMP7 Hyperproliferate in Response to Polyclonal Mitogens J. Immunol., April 1, 2006; 176(7): 4075 - 4082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Kisselev, A. Callard, and A. L. Goldberg Importance of the Different Proteolytic Sites of the Proteasome and the Efficacy of Inhibitors Varies with the Protein Substrate J. Biol. Chem., March 31, 2006; 281(13): 8582 - 8590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, A. F. Clark, and T. Yorio Heat Shock Protein 90 Is an Essential Molecular Chaperone for Nuclear Transport of Glucocorticoid Receptor {beta} Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 700 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Combaret, D. Dardevet, I. Rieu, M.-N. Pouch, D. Bechet, D. Taillandier, J. Grizard, and D. Attaix A leucine-supplemented diet restores the defective postprandial inhibition of proteasome-dependent proteolysis in aged rat skeletal muscle J. Physiol., December 1, 2005; 569(2): 489 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goralska, S. Nagar, L. N. Fleisher, and M. C. McGahan Differential Degradation of Ferritin H- and L-Chains: Accumulation of L-Chain-Rich Ferritin in Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3521 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-P. Kuang and R. H. Goldstein Regulation of elastin gene transcription by proteasome dysfunction Am J Physiol Cell Physiol, September 1, 2005; 289(3): C766 - C773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. York, E. P. Grant, A. M. Dahl, and K. L. Rock A Mutant Cell with a Novel Defect in MHC Class I Quality Control J. Immunol., June 1, 2005; 174(11): 6839 - 6846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Dorfel, S. Appel, F. Grunebach, M. M. Weck, M. R. Muller, A. Heine, and P. Brossart Processing and presentation of HLA class I and II epitopes by dendritic cells after transfection with in vitro-transcribed MUC1 RNA Blood, April 15, 2005; 105(8): 3199 - 3205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Rajkumar, P. G. Richardson, T. Hideshima, and K. C. Anderson Proteasome Inhibition As a Novel Therapeutic Target in Human Cancer J. Clin. Oncol., January 20, 2005; 23(3): 630 - 639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schlecht, J. Loucka, H. Najar, P. Sebo, and C. Leclerc Antigen Targeting to CD11b Allows Efficient Presentation of CD4+ and CD8+ T Cell Epitopes and In Vivo Th1-Polarized T Cell Priming J. Immunol., November 15, 2004; 173(10): 6089 - 6097. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-X. Wang, H.-M. Wang, Q.-L. Li, H.-Y. Lin, D. Qian, and C. Zhu Expression of Proteasome Subunits Low Molecular Mass Polypeptide (LMP) 2 and LMP7 in the Endometrium and Placenta of Rhesus Monkey (Macaca mulatta) During Early Pregnancy Biol Reprod, October 1, 2004; 71(4): 1317 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Krishnan and S. L. Zeichner Host Cell Gene Expression during Human Immunodeficiency Virus Type 1 Latency and Reactivation and Effects of Targeting Genes That Are Differentially Expressed in Viral Latency J. Virol., September 1, 2004; 78(17): 9458 - 9473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Albrecht and R. M. Williams Natural Product Synthesis Special Feature: A concise, total synthesis of the TMC-95A/B proteasome inhibitors PNAS, August 17, 2004; 101(33): 11949 - 11954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Gurnani, J. Kennedy, S. Sad, G. D. Sprott, and L. Krishnan Phosphatidylserine Receptor-Mediated Recognition of Archaeosome Adjuvant Promotes Endocytosis and MHC Class I Cross-Presentation of the Entrapped Antigen by Phagosome-to-Cytosol Transport and Classical Processing J. Immunol., July 1, 2004; 173(1): 566 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Lee, J. M. Brooks, H. Al-Jarrah, W. A. Thomas, T. A. Haigh, G. S. Taylor, S. Humme, A. Schepers, W. Hammerschmidt, J. L. Yates, et al. CD8 T Cell Recognition of Endogenously Expressed Epstein-Barr Virus Nuclear Antigen 1 J. Exp. Med., May 17, 2004; 199(10): 1409 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wujek, E. Kida, M. Walus, K. E. Wisniewski, and A. A. Golabek N-Glycosylation Is Crucial for Folding, Trafficking, and Stability of Human Tripeptidyl-peptidase I J. Biol. Chem., March 26, 2004; 279(13): 12827 - 12839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Fonteneau, D. G. Kavanagh, M. Lirvall, C. Sanders, T. L. Cover, N. Bhardwaj, and M. Larsson Characterization of the MHC class I cross-presentation pathway for cell-associated antigens by human dendritic cells Blood, December 15, 2003; 102(13): 4448 - 4455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vuocolo, E. Purev, D. Zhang, J. Bartek, K. Hansen, D. R. Soprano, and K. J. Soprano Protein Phosphatase 2A Associates with Rb2/p130 and Mediates Retinoic Acid-induced Growth Suppression of Ovarian Carcinoma Cells J. Biol. Chem., October 24, 2003; 278(43): 41881 - 41889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Moron, P. Rueda, C. Sedlik, and C. Leclerc In Vivo, Dendritic Cells Can Cross-Present Virus-Like Particles Using an Endosome-to-Cytosol Pathway J. Immunol., September 1, 2003; 171(5): 2242 - 2250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-F. Hung, W.-F. Cheng, L. He, M. Ling, J. Juang, C.-T. Lin, and T-C. Wu Enhancing Major Histocompatibility Complex Class I Antigen Presentation by Targeting Antigen to Centrosomes Cancer Res., May 15, 2003; 63(10): 2393 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Ott, L. V. Coren, R. C. Sowder II, J. Adams, and U. Schubert Retroviruses Have Differing Requirements for Proteasome Function in the Budding Process J. Virol., March 15, 2003; 77(6): 3384 - 3393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De, K. Jayarapu, L. Elenich, J. J. Monaco, R. A. Colbert, and T. A. Griffin beta 2 Subunit Propeptides Influence Cooperative Proteasome Assembly J. Biol. Chem., February 14, 2003; 278(8): 6153 - 6159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gnjatic, D. Atanackovic, M. Matsuo, E. Jager, S. Y. Lee, D. Valmori, Y.-T. Chen, G. Ritter, A. Knuth, and L. J. Old Cross-Presentation of HLA Class I Epitopes from Exogenous NY-ESO-1 Polypeptides by Nonprofessional APCs J. Immunol., February 1, 2003; 170(3): 1191 - 1196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. X. Cawley, Y. M. Rodriguez, A. Maldonado, and Y. P. Loh Trafficking of Mutant Carboxypeptidase E to Secretory Granules in a {beta}-Cell Line Derived from Cpefat/Cpefat Mice Endocrinology, January 1, 2003; 144(1): 292 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Robek, S. F. Wieland, and F. V. Chisari Inhibition of Hepatitis B Virus Replication by Interferon Requires Proteasome Activity J. Virol., December 2, 2002; 76(7): 3570 - 3574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Shanely, M. A. Zergeroglu, S. L. Lennon, T. Sugiura, T. Yimlamai, D. Enns, A. Belcastro, and S. K. Powers Mechanical Ventilation-induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity Am. J. Respir. Crit. Care Med., November 15, 2002; 166(10): 1369 - 1374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Chen, H. Kimura, and D. Schubert A novel mechanism for the regulation of amyloid precursor protein metabolism J. Cell Biol., July 8, 2002; 158(1): 79 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, A. J. A. M. Sijts, M. Song, K. Janek, A. K. Nussbaum, S. Kral, M. Schirle, S. Stevanovic, A. Paschen, H. Schild, et al. Expression of the Proteasome Activator PA28 Rescues the Presentation of a Cytotoxic T Lymphocyte Epitope on Melanoma Cells Cancer Res., May 1, 2002; 62(10): 2875 - 2882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Ott, L. V. Coren, R. C. Sowder II, J. Adams, K. Nagashima, and U. Schubert Equine Infectious Anemia Virus and the Ubiquitin-Proteasome System J. Virol., February 22, 2002; 76(6): 3038 - 3044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Botion, A. R. Brasier, B. Tian, V. Udupi, and A. Green Inhibition of Proteasome Activity Blocks the Ability of TNF{alpha} to Down-Regulate Gi Proteins and Stimulate Lipolysis Endocrinology, December 1, 2001; 142(12): 5069 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lautscham, S. Mayrhofer, G. Taylor, T. Haigh, A. Leese, A. Rickinson, and N. Blake Processing of a Multiple Membrane Spanning Epstein-Barr Virus Protein for CD8+ T Cell Recognition Reveals a Proteasome-dependent, Transporter Associated with Antigen Processing-independent Pathway J. Exp. Med., October 8, 2001; 194(8): 1053 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Abeliovich and D. J. Klionsky Autophagy in Yeast: Mechanistic Insights and Physiological Function Microbiol. Mol. Biol. Rev., September 1, 2001; 65(3): 463 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Potter and C. V. Harding Neutrophils Process Exogenous Bacteria Via an Alternate Class I MHC Processing Pathway for Presentation of Peptides to T Lymphocytes J. Immunol., September 1, 2001; 167(5): 2538 - 2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Luckey, J. A. Marto, M. Partridge, E. Hall, F. M. White, J. D. Lippolis, J. Shabanowitz, D. F. Hunt, and V. H. Engelhard Differences in the Expression of Human Class I MHC Alleles and Their Associated Peptides in the Presence of Proteasome Inhibitors J. Immunol., August 1, 2001; 167(3): 1212 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. van Kerkhof, C. M. A. dos Santos, M. Sachse, J. Klumperman, G. Bu, and G. J. Strous Proteasome Inhibitors Block a Late Step in Lysosomal Transport of Selected Membrane but not Soluble Proteins Mol. Biol. Cell, August 1, 2001; 12(8): 2556 - 2566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Vrana and S. Grant Synergistic induction of apoptosis in human leukemia cells (U937) exposed to bryostatin 1 and the proteasome inhibitor lactacystin involves dysregulation of the PKC/MAPK cascade Blood, April 1, 2001; 97(7): 2105 - 2114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Hilton, A. M. Dahl, and K. L. Rock Anti-Peptide Antibody Blocks Peptide Binding to MHC Class I Molecules in the Endoplasmic Reticulum J. Immunol., March 15, 2001; 166(6): 3952 - 3956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Princiotta, U. Schubert, W. Chen, J. R. Bennink, J. Myung, C. M. Crews, and J. W. Yewdell Cells adapted to the proteasome inhibitor 4-hydroxy- 5-iodo-3-nitrophenylacetyl-Leu-Leu-leucinal-vinyl sulfone require enzymatically active proteasomes for continued survival PNAS, January 5, 2001; (2001) 21132398. [Abstract] [Full Text]
|
|
|
|
|
|
A. Bai, C. J. Aldrich, and J. Forman Factors Controlling the Trafficking and Processing of a Leader-Derived Peptide Presented by Qa-1 J. Immunol., December 15, 2000; 165(12): 7025 - 7034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jackson, C. Harwood, M. Thomas, L. Banks, and A. Storey Role of Bak in UV-induced apoptosis in skin cancer and abrogation by HPV E6 proteins Genes & Dev., December 1, 2000; 14(23): 3065 - 3073. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Hilbi, R. J. Puro, and A. Zychlinsky Tripeptidyl Peptidase II Promotes Maturation of Caspase-1 in Shigella flexneri-Induced Macrophage Apoptosis Infect. Immun., October 1, 2000; 68(10): 5502 - 5508. [Abstract] [Full Text] [PDF]
|
|
|
|
